Analytical Resources Core

分析资源核心

• 批准号: 9173322
• 负责人: MACRAE F LINTON
• 金额: $ 28.73万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9173322
• 关键词: Amino Acids; Animals; Arterial Fatty Streak; Atherosclerosis; Biological Assay; Biological Models; Body Weight; Carotid Arteries; Cholesterol; Citric Acid Cycle; Communities; Conscious; Contract Services; Endocrine; Equipment; Fatty Acids; Fee-for-Service Plans; Fostering; Glucose; Goals; Health; Histology; Histopathology; Hormones; Human Resources; In Vitro; Individual; Laboratories; Lesion; Lipids; Lipoproteins; Mass Fragmentography; Measurement; Measures; Metabolic; Metabolic Diseases; Metabolic Pathway; Methods; Mission; Modeling; Modification; Mus; Nonesterified Fatty Acids; Pathologist; Pathology; Pathway interactions; Plasma; Reagent; Recording of previous events; Regulation; Research; Research Activity; Research Personnel; Resources; Sample Size; Sampling; Scientist; Services; Shipping; Ships; Social Welfare; Specificity; System; Techniques; Tissue Microarray; Tissues; Training and Education; Triglycerides; Wages; Work; aortic arch; cost; cost effective; cytokine; digital; improved; in vitro Model; in vivo; laser capture microdissection; meetings; metabolic phenotype; method development; mouse model; novel

The Analytical Resources Core (ARC) has been an integral part of the VMMPC since it was founded in 2001. The ARC will consist of four subcores: 1) Hormone Assay and Analytical Services; 2) Lipids, Lipoproteins, and Atherosclerosis (LLA); 3) Mouse Pathology; and 4) Metabolic Flux Analysis (MFA). Throughout its history the ARC has operated at capacity with usage by investigators both inside and outside Vanderbilt. The ARC will undergo two major changes this cycle. The first change is that mouse atherosclerosis and plaque composition analyses will be moved to the ARC where they will be part of the Lipids, Lipoproteins and Atherosclerosis (LLA) Subcore. The second modification to the ARC is the creation of the Metabolic Flux Analysis (MFA) Subcore. The MFA Subcore involves the use of newly developed stable isotopic methods to assess metabolic flux in the conscious mouse or in vitro model systems. The goals of the core are to provide controlled, precision services that streamline research activities, produce cost-effective lines of experimentation, foster collaborative enterprises, and provide alternative outlets to scientists reaching the technical limits of their own laboratories. The services offered by each subcore are unique in their application to the mouse, and great effort has been made to establish assay specificity and scale down sample size to accommodate samples from this species. The Core provides space, equipment, and personnel for sample analyses and methods development. Investigators pay a fee-for-service that covers the cost of reagents, supplies, a percentage of personnel salary, and service contracts.

自2001年成立以来，分析资源核心（ARC）一直是VMMPC的组成部分。 ARC将由四个子核组成：1）激素分析和分析服务； 2）脂质，脂蛋白和 动脉粥样硬化（LLA）； 3）小鼠病理； 4）代谢通量分析（MFA）。整个历史 ARC在范德比尔特内部和外部的调查人员使用方面运作。弧度会 在这个周期中经历了两个重大变化。第一个变化是小鼠动脉粥样硬化和斑块组成 分析将移至弧度，在那里它们将成为脂质，脂蛋白和动脉粥样硬化的一部分 （LLA）子核。对弧的第二个修改是创建代谢通量分析（MFA） 子核。 MFA子核心涉及使用新开发的稳定同位素方法来评估代谢 意识小鼠或体外模型系统中的通量。核心的目标是提供受控的， 精简研究活动，产生具有成本效益的实验线的精确服务，寄养 协作企业，并为科学家提供替代渠道，以达到自己的技术极限 实验室。每个子核心提供的服务在其应用于鼠标的应用中是独一无二的，而且很棒 已经努力建立分析特异性并缩小样本量以适应来自 这个物种。核心提供了用于样本分析和方法的空间，设备和人员 发展。调查人员支付涵盖试剂，供应费用，百分比的费用的费用。 人员工资和服务合同。