Pilot Project Core

试点项目核心

• 批准号: 9151762
• 负责人: Elissa J Chesler
• 金额: $ 21.63万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-15 至 2021-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9151762
• 关键词: Address; Alleles; Applications Grants; Area; Award; Behavior; Behavioral; Biology; Budgets; Chromatin; Cocaine; Communities; Consultations; Data; Development; Engineering; Ensure; Epigenetic Process; Evaluation; Feedback; Foundations; Funding; Future; Generations; Genes; Genetic; Genetic Polymorphism; Genetic Techniques; Genetic Variation; Genetic study; Genomic medicine; Genomics; Goals; Human; Human Genetics; Inbreeding; Laboratory mice; Lead; Modeling; Molecular; Monitor; Mouse Strains; Mus; Pharmaceutical Preparations; Phase; Phenotype; Pilot Projects; Process; Quantitative Trait Loci; Research; Research Personnel; Resources; Risk; Schedule; Scientist; Self Administration; Series; Services; Structure; System; Techniques; Technology; Testing; The Jackson Laboratory; Touch sensation; Transgenic Organisms; Variant; abstracting; addiction; base; biobehavior; career; design; forging; genetic approach; genetic resource; human genomics; innovation; insight; meetings; member; methamphetamine use; model development; mouse model; neurobehavioral; neurogenetics; novel; planetary Atmosphere; programs; research study; technological innovation; trait; transcriptomics; translational genetics; web portal

PROJECT SUMMARY/ABSTRACT PILOT PROJECT CORE The Center for Systems Neurogenetics of Addiction (CSNA) will offer pilot project opportunities to support the development of future CSNA-affiliated projects. Pilot projects will create critical opportunities for new investigators to generate key preliminary data for future systems genetic studies of addiction and will enable established investigators to test new hypotheses and migrate their research into the use of novel mouse genetic platforms for the assessment of biobehavioral addiction risk. In the initial phase of the program, we have selected three pilot projects. The first uses the phenomenal genetic diversity in the CC/DO inbred founders to test for modifiers of a new cocaine sensitization quantitative trait gene recently discovered by QTL analysis in closely related mouse strains, Cyfip2, and makes use of the CSNA Behavioral Phenotyping Core to extrapolate the effects of this polymorphism from cocaine sensitization to behavior in drug self-administration paradigms. The second pilot project comes from an established human geneticist. This is a translational genetics project, aimed to model and characterized methamphetamine use-related variants discovered in human genetic studies of addiction. Candidate variants will be engineered into the laboratory mouse using BAC transgenic strategies. These induced variants will be evaluated through a series of behavioral and molecular experiments. A third pilot project will enable integration of the CSNA's transcriptomic and genetic findings with epigenetic analysis by evaluating open chromatin in cocaine treated and control mice from the CC/DO founder strains. Future pilot projects will be selected from an open solicitation to the addiction research community and will be selected by the Internal Advisory Board in consultation with the External Advisory Board through a process coordinated by the Administrative Core. Criteria for the projects include overall impact, innovation, investigator, approach and feasibility within the CSNA, significance and relevance to the center, and likelihood to develop into center-affiliated, funded projects.

项目摘要/摘要飞行项目核心 系统神经源性成瘾中心（CSNA）将为试点项目提供支持 开发未来CSNA附属项目。试点项目将为新的机会创造关键机会 研究人员为成瘾的未来系统遗传研究生成关键的初步数据，并将启用 建立的研究人员来检验新的假设并将其研究迁移到新型鼠标的使用中 用于评估生物行为成瘾风险的遗传平台。在程序的初始阶段，我们 选择了三个飞行员项目。第一个使用CC/DO近交的现象遗传多样性 创始人测试QTL最近发现的新可卡因敏化定量性状基因的修饰符 在密切相关的小鼠菌株Cyfip2中分析，并利用CSNA行为表型核心 推断从可卡因敏化到药物自我给药的行为的这种多态性的影响 范式。第二个试点项目来自既定的人类遗传学家。这是翻译 遗传学项目，旨在建模和表征甲基苯丙胺使用相关的变体 人类成瘾的遗传研究。候选变体将使用实验室鼠标进行设计 BAC转基因策略。这些诱导的变体将通过一系列行为和 分子实验。第三个试点项目将使CSNA的转录组和遗传融合 通过评估来自可卡因的开放染色质和对照小鼠的开放性染色质的发现 CC/DO创始人菌株。未来的飞行员项目将从开放的招标中选择到成瘾研究 社区，将由内部顾问委员会与外部咨询委员会协商 通过由行政核心协调的过程。这些项目的标准包括整体影响， CSNA内的创新，研究人员，方法和可行性，与中心的意义和相关性， 并有可能发展成中心附属的资助项目。