CYTOGENETICS

细胞遗传学

• 批准号: 9061617
• 负责人: CONSTANCE A GRIFFIN
• 金额: $ 6.59万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9061617
• 关键词: Cancer cell line; Chromosome Structures; Chromosome abnormality; Chromosomes; Comprehensive Cancer Center; Consultations; Cytogenetic Analysis; Cytogenetics; Cytogenetics Shared Resource; DNA; Data; Fluorescent in Situ Hybridization; Foundations; G-Banding; Genomics; Human Resources; In Situ Hybridization; Malignant Neoplasms; Metaphase; Research; Research Personnel; Research Project Grants; Resources; SNP array; Services; Spectral Karyotyping; Techniques; Technology; Tissue Microarray; Tissues; chromatin immunoprecipitation; experience; oncology; programs

Cancer cytogenetics expertise is needed to provide critical data for research projects in many Programs of the Sidney Kimmel Comprehensive Cancer Center (SKCCC). The Cancer Cytogenetics Shared Resource has been providing such expertise to SKCCC researchers since 1986. A full array of analyses is offered; these include metaphase analysis using traditional G-banding and fluorescent in situ hybridization (FISH) technologies, ranging from single locus probes through multicolor FISH including Spectral Karyotyping (SKY). Consultations to determine the best cytogenetic technique to answer the investigator's question are an integral part of the service provided. Up-to-date techniques and highly experienced personnel have been the foundation of this facility and provide major value to the users. The Core also coordinates with services offered by the Tissue Microarray Core, such as tissue in-situ hybridization, and the Microarray Core, such as genomic copy-number and SNP-chip assays, to provide a seamless continuum of genomic analysis technologies in the SKCCC. Lay: The Cytogenetics Core Resource provides expert analysis of chromosomes, the structures that carry our DNA. Chromosome abnormalities in cancer are common, and researchers often need characterization of chromosome changes that are present in cancer cell lines.

需要在Sidney Kimmel综合癌症中心（SKCCC）的许多计划中为研究项目提供关键数据的癌症细胞遗传学专业知识。自1986年以来，癌症细胞遗传学共享资源一直向SKCCC研究人员提供此类专业知识。提供了一系列分析。这些包括使用传统的G带和荧光原位杂交（FISH）技术的中期分析，从单基因座探针到包括光谱核分型（SKY）的多色鱼类。确定回答研究者问题的最佳细胞遗传学技术的咨询是所提供的服务的组成部分。最新技术和经验丰富的人员一直是该设施的基础，并为用户提供了主要价值。核心还与组织微阵列核心提供的服务（例如组织原位杂交杂交）以及微阵列核心（例如基因组拷贝数和SNP-CHIP测定法）提供了无缝基因组分析技术中的无缝连续性。 Lay：细胞遗传学核心资源提供了染色体的专家分析，即携带DNA的结构。癌症的染色体异常很常见，研究人员通常需要表征癌细胞系中存在的染色体变化。