Single Cell and Immunoanalysis Core

单细胞和免疫分析核心

基本信息

批准号：
10615219
负责人：
JAMES A. LEDERER
金额：
$ 14.11万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-07-19 至 2026-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10615219
关键词：
ATAC-seq Acceleration Antibodies Biological Models Biology Biometry Biopsy Cells Clinical Trials Clone Cells Cryopreservation Custom Cytometry DNA Data Analyses Distant Experimental Designs Flow Cytometry Geographic Locations Human Human Biology Image Immune Immune response Immunobiology Inflammatory Institution Location Pathogenicity Patients Preparation Proteins Protocols documentation Rare Earth Metals Reagent Research Research Design Research Personnel Research Project Grants Resources Sampling Sampling Studies Services Shipping Skin Stains T cell receptor repertoire sequencing T-Cell Receptor Genes T-Lymphocyte Techniques Time Tissues Transgenic Mice Transgenic Model advanced analytics antibody conjugate cellular imaging cost image reconstruction insight mouse model next generation service utilization single cell sequencing single-cell RNA sequencing skin disorder

项目摘要

Project Summary/Abstract: The Single Cell and Immunoanalysis Core Research into human skin biology and inflammatory skin diseases has been slowed by the lack of transgenic model systems and the limited samples that can be obtained from living patients. However, advanced analytic techniques that provide maximal information from small samples have been developed that have the potential to advance our understanding of human skin disease. This Core makes critical groundbreaking techniques including single cell RNA sequencing (scRNA-seq) with the ability to batch samples, discover TCR genes and tag cells with antibodies (CITE-seq), as well as single cell ATAC-seq, single cell imaging by flow cytometry, cytometry by time of flight (CyTOF) and high throughput T cell receptor sequencing available to investigators at any institution who wish to carry out human skin disease research. The Center also provides assistance with experimental planning, key reagents and assistance with data analysis for each technique. For example, the Center provide protocols that allow skin biopsies to be shipped from distant locations and viably cryopreserved before scRNA-seq studies; this advance makes single cell profiling available to investigators to study samples from clinical trials and from understudied skin diseases. The Center also provides CyTOF staining protocols, validated panels of CyTOF antibodies, custom conjugation of CyTOF antibodies, advice on experimental design and data analysis, and all services needed for outside investigators to send their cells to the Center for CyTOF studies. The Center provides comprehensive support in the experimental design, DNA extraction protocols, DNA sample preparation services, shipping, and data analysis assistance that will enable researchers to successfully utilize high throughput TCR sequencing to profile skin immune responses and track pathogenic T cell clones across multiple timepoints and tissues. We have included 36 research projects from investigators who wish to utilize the services of this Core. In summary, this core provides access to single cell approaches that have the potential to provide unprecedented insights into the biology of human skin disease.

项目摘要/摘要：单细胞和免疫分析核心由于缺乏转基因技术，对人类皮肤生物学和炎症性皮肤病的研究已经放缓模型系统和可以从活体患者获得的有限样本。然而，高级分析已经开发出从小样本提供最大信息的技术，具有潜力增进我们对人类皮肤病的了解。该核心具有关键的突破性技术包括单细胞 RNA 测序 (scRNA-seq)，能够批量样品、发现 TCR 基因和用抗体标记细胞 (CITE-seq)，以及单细胞 ATAC-seq、通过流式细胞术进行单细胞成像，研究人员可使用飞行时间流式细胞术 (CyTOF) 和高通量 T 细胞受体测序任何希望进行人类皮肤病研究的机构。该中心还提供以下方面的帮助：每种技术的实验计划、关键试剂和数据分析协助。例如，该中心提供允许从遥远的地方运送皮肤活检并进行可行冷冻保存的协议在 scRNA-seq 研究之前；这一进步使研究人员可以利用单细胞分析来研究样本来自临床试验和未充分研究的皮肤病。该中心还提供CyTOF染色方案，经过验证的 CyTOF 抗体组、CyTOF 抗体的定制缀合、实验建议设计和数据分析，以及外部研究人员将细胞送到中心进行研究所需的所有服务 CyTOF 研究。中心在实验设计、DNA提取等方面提供全面支持方案、DNA 样品制备服务、运输和数据分析协助，使研究人员成功利用高通量 TCR 测序来分析皮肤免疫反应，跨多个时间点和组织追踪致病性 T 细胞克隆。我们纳入了 36 个研究项目来自希望利用该核心服务的调查人员。总之，该核心提供对单个细胞方法有可能为人类皮肤生物学提供前所未有的见解疾病。