PNNL Clinical Proteome Characterization Center
PNNL 临床蛋白质组表征中心
基本信息
- 批准号:9301181
- 负责人:
- 金额:$ 117.04万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-08-01 至 2017-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The Overall Objective ofthe PNNL Clinical Proteome Characterization Center
(PCPCC) is to facilitate cancer biomarker development by linking the cancer genotype to the cancer phenotype
using detailed comprehensive and quantitative characterization of cancer proteomes to complement the extensive
genome-level characterization provided by The Cancer Genome Atlas (TCGA). The PCPCC will contribute to the
success ofthe planned network of Protein Characterization Centers (PPCs) by utilizing robust and quantitative
proteomics technologies and workflows, including simultaneous application of state-of-the-art validated platforms
and advanced developmental platforms, for systematic discovery and verification of protein biomarkers that can
be qualified in clinical studies, using the cancer specimens and associated data provided by the CPTC.
The Discovery Unit will make measurements providing a comprehensive and quantitative characterization ofthe
cancer proteomes that provides Information including protein abundances, splicing variants, mutations, and
posttranslational modifications to complement the genomic characterization for CPTC-supplied biospecimens.
The extensive database of genomic information on these samples will be integrated with the quantitative
proteomic measurements made by the PCPCC, other available proteomics Information (e.g., from other PCC's),
and a systems-level analysis of tumor-speclfic pathways to produce a prioritized list of highly credentialed
candidates based on a weighted integration of multiple sources of Information, including clinical oncology and
cancer biology.
The Verification Unit will systemically develop and apply multiplexed verification assays directed at specific protein
targets as identified and selected by the Biomarker Candidate Selection Subcommittee. The PCPCC will develop
sensitive, selective, quantitative assays for a minimum of 100 protein targets per year and apply ultra-sensitive
assays to biomari<er verification with a throughput of at least 500 plasma (or serum) samples per year, for a total
of at least 2500 samples. Additionally, as in the Discovery efforts, measurements with the best available validated
platform will be augmented by measurements with a developmental high performance platform for the same
samples (for an overall total of at least 1000 samples per year, and >5000 total) to provide quantitative
measurements for low-abundance otherwise undetectable candidates.
As part of a consortium of PCC's, the PCPCC will also work to advance the efforts of others based upon e.g. the
cancer tumor proteomics data generated, as well as subsequent biomarker clinical qualification and validation
efforts.
RELEVANCE (See instructions): Despite recent declines in the cancer death rate, cancer remains a significant
source of mortality: 25% of all deaths In the US are attributed to cancer. There Is a real clinical need for eariier
diagnosis, more accurate prognosis, and more effective therapeutic targeting to improve the outcome for patients
with cancer. The PCPCC is dedicated to using the best state-of-the-art and advanced developmental MS
platforms and methods, systems biology approaches, and clinical collaborations, to advance the discovery and
verification of cancer biomarkers.
PNNL临床蛋白质组表征中心的总体目标
(PCPCC)是通过将癌症基因型与癌症表型联系起来来促进癌症生物标志物的发展
使用癌症蛋白质组的详细综合和定量表征来补充广泛的
癌症基因组图集(TCGA)提供的基因组水平表征。 PCPCC将有助于
通过使用鲁棒和定量的计划蛋白质表征中心(PPC)的成功网络的成功
蛋白质组学技术和工作流程,包括同时应用最先进的验证平台
和先进的发展平台,用于系统的发现和验证蛋白质生物标志物
使用CPTC提供的癌症标本和相关数据,在临床研究中有资格。
发现单元将进行测量,从而提供全面和定量的表征
提供信息,包括蛋白质丰度,剪接变体,突变和
翻译后修饰,以补充CPTC供供培养物的基因组表征。
这些样品的基因组信息的广泛数据库将与定量集成
PCPCC进行的蛋白质组学测量,其他可用的蛋白质组学信息(例如,来自其他PCC),
以及对肿瘤特定途径的系统级分析,以生成高度凭证的优先级列表
基于多种信息来源的加权整合,包括临床肿瘤学和
癌症生物学。
验证单元将系统地开发和应用针对特定蛋白质的多路复用验证测定法
由生物标志物候选者选择小组委员会确定和选择的目标。 PCPCC将开发
敏感,选择性,定量测定最少每年100个蛋白质靶标,并应用超敏感性
每年至少有500个血浆(或血清)样品的吞吐量,对生物组织进行测定,总计
至少2500个样品。此外,与发现工作一样,具有最佳可用验证的测量值
平台将通过使用开发高性能平台进行的测量来增强平台
样本(每年至少总计至少1000个样本,总计> 5000个样本),以提供定量
低丰度的测量,原本无法检测到的候选人。
作为PCC财团的一部分,PCPCC还将努力根据例如这
产生的癌症肿瘤蛋白质组学数据以及随后的生物标志物临床资格和验证
努力。
相关性(请参阅说明):尽管最近癌症死亡率下降,但癌症仍然是重要的
死亡率来源:美国所有死亡人数中有25%归因于癌症。对Eariier有真正的临床需求
诊断,更准确的预后和更有效的治疗靶向,以改善患者的预后
癌症。 PCPCC致力于使用最好的最新和先进的发展性MS
平台和方法,系统生物学方法和临床合作,以提高发现和
验证癌症生物标志物。
项目成果
期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Integrative genomics and computational systems medicine.
- DOI:10.1155/2014/945253
- 发表时间:2014
- 期刊:
- 影响因子:0
- 作者:McDermott JE;Huang Y;Zhang B;Xu H;Zhao Z
- 通讯作者:Zhao Z
The utility of protein and mRNA correlation.
- DOI:10.1016/j.tibs.2014.10.010
- 发表时间:2015-01
- 期刊:
- 影响因子:13.8
- 作者:Payne SH
- 通讯作者:Payne SH
Challenges in Biomarker Discovery: Combining Expert Insights with Statistical Analysis of Complex Omics Data.
- DOI:10.1517/17530059.2012.718329
- 发表时间:2013-01
- 期刊:
- 影响因子:0
- 作者:McDermott JE;Wang J;Mitchell H;Webb-Robertson BJ;Hafen R;Ramey J;Rodland KD
- 通讯作者:Rodland KD
Cardiotoxicity of Antineoplastic Therapies and Applications of Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
- DOI:10.3390/cells10112823
- 发表时间:2021-10-21
- 期刊:
- 影响因子:6
- 作者:Huang MF;Pang LK;Chen YH;Zhao R;Lee DF
- 通讯作者:Lee DF
Proteomics of ovarian cancer: functional insights and clinical applications.
- DOI:10.1007/s10555-014-9547-8
- 发表时间:2015-03
- 期刊:
- 影响因子:0
- 作者:Elzek MA;Rodland KD
- 通讯作者:Rodland KD
共 7 条
- 1
- 2
Karin D Rodland的其他基金
Support for US HUPO Meeting "Future of Proteomics"
支持美国HUPO会议“蛋白质组学的未来”
- 批准号:83199428319942
- 财政年份:2012
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
Deep Proteomics of Normal Human Ovarian Surface Epithelium and Fallopian Tube Epi
正常人卵巢表面上皮和输卵管上皮的深层蛋白质组学
- 批准号:87908278790827
- 财政年份:2011
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
Center for Application of Advanced Clinical Proteomic Technologies for Cancer
先进癌症临床蛋白质组学技术应用中心
- 批准号:85211928521192
- 财政年份:2011
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
Center for Application of Advanced Clinical Proteomic Technologies for Cancer
先进癌症临床蛋白质组学技术应用中心
- 批准号:88965218896521
- 财政年份:2011
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
Center for Application of Advanced Clinical Proteomic Technologies for Cancer
先进癌症临床蛋白质组学技术应用中心
- 批准号:83245918324591
- 财政年份:2011
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
Center for Application of Advanced Clinical Proteomic Technologies for Cancer
先进癌症临床蛋白质组学技术应用中心
- 批准号:81534938153493
- 财政年份:2011
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
REGULATION OF OVARIAN SURFACE EPITHELIAL CELLS
卵巢表面上皮细胞的调节
- 批准号:69706156970615
- 财政年份:2004
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
CALCIUM RECEPTORS AND PROLIFERATIVE SIGNALS
钙受体和增殖信号
- 批准号:65543316554331
- 财政年份:1998
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
CALCIUM SIGNALING AND OVARIAN CANCER
钙信号传导与卵巢癌
- 批准号:61740696174069
- 财政年份:1998
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
CALCIUM SIGNALING AND OVARIAN CANCER
钙信号传导与卵巢癌
- 批准号:65543466554346
- 财政年份:1998
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
相似国自然基金
新型活性酯靶向赖氨酸的活细胞化学标记方法及其生物学应用
- 批准号:22307084
- 批准年份:2023
- 资助金额:30 万元
- 项目类别:青年科学基金项目
元素标记单颗粒等离子体质谱数字检测新方法及其生物分析应用
- 批准号:22374111
- 批准年份:2023
- 资助金额:50 万元
- 项目类别:面上项目
基于2bRAD-M技术探索尸体微生物基因组分子标记的时相变化规律及法医学应用
- 批准号:82371896
- 批准年份:2023
- 资助金额:49 万元
- 项目类别:面上项目
基于深度学习的重度抑郁症亚型识别和个体化生物标记解析研究
- 批准号:62301549
- 批准年份:2023
- 资助金额:30 万元
- 项目类别:青年科学基金项目
膝关节软骨退变多模态磁共振成像与软骨及滑膜相关生物标记物表达关系的实验研究
- 批准号:82360339
- 批准年份:2023
- 资助金额:32 万元
- 项目类别:地区科学基金项目
相似海外基金
Pre-clinical Bruker Albira Si PET/SPECT/CT imaging system
临床前 Bruker Albira Si PET/SPECT/CT 成像系统
- 批准号:1063302210633022
- 财政年份:2023
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
CAR T cells targeting mesothelin and secreting bispecific antibodies targeting fibroblasts in pancreatic cancer
CAR T 细胞靶向间皮素并分泌靶向胰腺癌成纤维细胞的双特异性抗体
- 批准号:1073163510731635
- 财政年份:2023
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
High resolution profiling of cellular communities in the tumor microenvironment
肿瘤微环境中细胞群落的高分辨率分析
- 批准号:1057235510572355
- 财政年份:2023
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
Molecularly Targeted Probes for Photodynamic Therapy and Imaging of Breast Cancer
用于乳腺癌光动力治疗和成像的分子靶向探针
- 批准号:1073838810738388
- 财政年份:2023
- 资助金额:$ 117.04万$ 117.04万
- 项目类别:
Improving response prediction to neoadjuvant therapy in pancreatic cancer
改善胰腺癌新辅助治疗的反应预测
- 批准号:1078427210784272
- 财政年份:2023
- 资助金额:$ 117.04万$ 117.04万
- 项目类别: