Regulation and Physiological Roles of Translational Fidelity

翻译保真度的调节和生理作用

基本信息

批准号：
10617051
负责人：
JIQIANG LING
金额：
$ 6.48万
依托单位：
UNIV OF MARYLAND, COLLEGE PARK
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10617051
关键词：
Affect African American population Antibiotics Bacteria Bacteria sigma factor KatF protein Biochemical Biochemistry Biomedical Research Candidate Disease Gene Cell Culture Techniques Cells Cellular biology Communicable Diseases Critical Pathways Development Ensure Evolution Experimental Designs Five-Year Plans Fluorescence Future Genes Genetic Goals Hospitalization Impairment Infection International Knock-out Knowledge Libraries Mass Spectrum Analysis Microbiology Minority Groups Modeling Molecular Molecular Biology Mutation Nutrient Osmolalities Outcome Pathway interactions Physiological Population Protein Biosynthesis Quality Control Reading Regulation Reporter Research Resolution Ribosomes Role Salmonella Salmonella enterica Scientist Stress Students System Systems Biology Temperature Terminator Codon Testing Thinking Training Translations United States Western Blotting Work Writing bacterial genetics biological adaptation to stress career development career networking cell motility cost effective doctoral student empowered environmental stressor global health high throughput screening interest lambda Spi-1 macrophage meetings microbial new technology novel parent grant pathogen response single cell analysis skills student mentoring therapy development transmission process underrepresented minority student

项目摘要

PROJECT SUMMARY The goal of this project is to enhance the diversity of the biomedical research workforce by training a Ph.D. student from an underrepresented minority group (African Americans) and facilitate her development towards an independent scientist in the field of microbiology and pathogen-host interactions. The student will receive rigorous training to study pathogen-host interactions, protein synthesis, bacterial genetics, and microbial stress responses. Translational fidelity is critically maintained throughout evolution to ensure accurate protein synthesis. However, the role of translational fidelity in pathogen-host interactions remains largely unknown. The proposed training aims at studying how translational fidelity affects Salmonella-macrophage interactions. During the training, the student will (a) develop important research skills covering bacterial genetics, single-cell analyses, cell culture, molecular biology, and biochemistry, (b) enhance her skills of critical reading, thinking, presentation, and writing, and (c) strengthen her ability of proper experimental design and development of hypothesis-driven research. In addition, the student will have the opportunities to further career development by engaging in presenting research at local/national/international meetings, mentoring students, and taking professional training courses. The outcomes of the proposed training will help elucidate the role of translational fidelity in pathogen-host interactions using Salmonella as a model, further the student’s development as an independent microbiologist, expand the student’s professional network, and promote diversity in the workforce of microbiology and infectious diseases.

项目概要该项目的目标是通过以下方式增强生物医学研究人员的多样性培养来自少数群体的博士生（非裔美国人）并促进她成为该领域的独立科学家微生物学和病原体-宿主相互作用的学生将接受严格的培训。研究病原体-宿主相互作用、蛋白质合成、细菌遗传学和微生物应激在整个进化过程中严格保持翻译保真度，以确保准确的反应。然而，翻译保真度在病原体-宿主相互作用中的作用。拟议的培训旨在研究翻译保真度如何。影响沙门氏菌-巨噬细胞相互作用在训练期间，学生将 (a) 发展。重要的研究技能，涵盖细菌遗传学、单细胞分析、细胞培养、分子生物学生物学和生物化学，(b) 提高她的批判性阅读、思考、表达和表达的技能写作，以及（c）加强她正确的实验设计和开发的能力此外，学生将有机会进一步发展职业生涯。通过在地方/国家/国际会议上展示研究成果来促进发展，指导学生并参加专业培训课程。培训将有助于阐明翻译保真度在病原体-宿主相互作用中的作用沙门氏菌作为模型，进一步促进学生作为独立微生物学家的发展，扩大学生的专业网络，促进劳动力的多样性微生物学和传染病。