Selenium metabolism in cold-induced adaptive thermogenesis

冷诱导适应性产热中的硒代谢

• 批准号: 10616992
• 负责人: Lucia Andreia Seale
• 金额: $ 4.81万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10616992
• 关键词: Adipocytes; Administrative Supplement; Bioinformatics; Brown Fat; Cell Death; Complement; Development Plans; Doctor of Philosophy; Educational workshop; Enzymes; Experimental Designs; Genes; Hispanic; Histology; Iron; Knockout Mice; Medical; Metabolism; Micronutrients; Microscopy; Oral; Parents; Recycling; Research; Research Training; Role; Selenium; Thermogenesis; Training; Underrepresented Minority; Writing; adipocyte differentiation; career; career development; collaborative environment; experience; in vivo; meetings; mouse model; parent grant; programs; research and development; response; selenocysteine lyase; selenoprotein; skills

ABSTRACT The studies proposed in this Administrative Supplement to parent grant DK128390 aim to promote the scientific research and career development of Sydonie Swanson, a Postbaccalaureate candidate with a Hispanic heritage seeking to gain research experience. The parent grant focuses on determining the role of selenium recycling in regulating selenoprotein enzymes GPX1 and DIO2 in brown adipose tissue and how the recycling mechanism contributes to cold-induced adaptive thermogenesis in vivo, using whole-body and brown adipocyte-specific knockout mouse models of the selenium recycling enzyme selenocysteine lyase (SCLY). This Administrative Supplement will complement these studies by pursuing these Research Aims: 1) To determine the role of selenium and SCLY in the differentiation of brown adipocytes; and 2) To determine if treatment of pre-adipocytes with selenium or the activity of SCLY controls the expression and activity of selenoprotein GPX4, impacting responses to ferroptosis, an iron-dependent cell death mechanism, in differentiated brown adipocytes. The Research and Career Development Plan for Sydonie focuses on training technical (e.g., histology, microscopy, gene editing, bioinformatics) and professional (e.g., experimental design, scientific writing, and oral presentations) skills in a collaborative environment that will provide a robust research experience and favor her successful inclusion in the medical workforce.

抽象的 本家长补助金 DK128390 行政补充中提出的研究旨在促进科学 Sydonie Swanson 的研究和职业发展，一名具有西班牙裔血统的学士后候选人 寻求获得研究​​经验。母基金的重点是确定硒回收在 棕色脂肪组织中硒蛋白酶GPX1和DIO2的调控及其回收机制 利用全身和棕色脂肪细胞特异性，有助于体内冷诱导的适应性产热 硒回收酶硒代半胱氨酸裂解酶（SCLY）的敲除小鼠模型。本行政 增刊将通过追求以下研究目标来补充这些研究：1) 确定 硒和SCLY在棕色脂肪细胞分化中的作用； 2) 确定是否治疗前脂肪细胞 硒或 SCLY 的活性控制硒蛋白 GPX4 的表达和活性，影响 对铁死亡的反应， 分化的棕色脂肪细胞中的铁依赖性细胞死亡机制。这 Sydonie 的研究和职业发展计划侧重于技术培训（例如组织学、显微镜、 基因编辑、生物信息学）和专业（例如实验设计、科学写作和口语） 演示）协作环境中的技能，这将提供丰富的研究经验并有利于她 成功融入医疗队伍。