ShEEP Request for Zeiss LSM880 Confocal Microscope

ShEEP 请求蔡司 LSM880 共焦显微镜

• 批准号: 9210692
• 负责人: Lee-Ann H Allen
• 金额: --
• 依托单位: IOWA CITY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9210692
• 关键词: 18 year old; ASIC channel; Achievement; Alzheimer' s Disease; Anxiety; Anxiety Disorders; Apoptosis; Autophagocytosis; B-Lymphocytes; Bacterial Infections; Biological Process; Blindness; Brain; Cell Death; Cell physiology; Cells; Cities; Collaborations; Color; Communicable Diseases; Computer software; Confocal Microscopy; Country; Cytoplasmic Granules; DNA; Data; Data Collection; Detection; Development; Diabetes Mellitus; Disease; Ensure; Equipment; Fluorescence; Fluorescence Resonance Energy Transfer; Francisella tularensis; Funding; Glaucoma; Glia Maturation Factor; Health; Heart; Heart Diseases; Helicobacter Infections; Housing; Image; Image Analysis; Immune system; Immunity; Infection; Inflammation; Inflammatory; Invaded; Iowa; Laboratories; Lasers; Leishmania infantum; Life; Light; Lighting; Maintenance; Medical center; Mental Depression; Microbe; Microbial Biofilms; Microscope; Mitochondria; Modeling; Morphologic artifacts; Movement; Mutate; Noise; Optics; Organelles; Parasites; Parkinson Disease; Pathogenesis; Patients; Phagosomes; Play; Positioning Attribute; Post-Traumatic Stress Disorders; Productivity; Program Evaluation; Proteins; Publications; Request for Applications; Research; Research Project Grants; Resolution; Retinal Ganglion Cells; Role; Running; Sampling; Scanning; Scientist; Series; Signal Transduction; Site; Skin; Speed; Staphylococcus aureus; Support System; Synaptic plasticity; System; TNF receptor-associated factor 3; Technology; Therapeutic Intervention; Thick; Time; Tissue Sample; Tissues; Veterans; Visible Radiation; cancer cell; cell fixing; cell motility; computer monitor; detector; discount; exosome; flexibility; gallium arsenide; improved; insight; instrument; interest; macrophage; microvesicles; nervous system disorder; neutrophil; optical spectra; photomultiplier; programs; quasar; trafficking

With this application we request funds from the ORD Shared Equipment Evaluation Program (SheEEP) to purchase a new confocal microscope to be housed in the Inflammation Program laboratories of the Iowa City VA Medical Center. Specifically, we seek to obtain a model LSM 880 confocal microscope manufactured by Carl Zeiss, Inc. The rationale for this purchase is threefold. First, our current confocal microscope, a Zeiss LSM 510, is 18 years old, and as of September 2016 will be officially obsolete. As such, parts and maintenance will no longer be available, and the instrument, which plays a critical role in many research programs, will become unusable. Second, we have the opportunity to take advantage of a 20% trade-up discount on purchase of the LSM 880. Third and most importantly, there are several features of the LSM 880 that place it at the forefront of confocal technology. Thus, acquisition of an LSM 880 will support cutting edge research and will allow the development of new research projects and new collaborations among scientists of the Iowa City VA research program, which has a long-standing record of excellence and achievement. Our research portfolio is diverse and includes studies of several infectious diseases, inflammation and immunity, glaucoma and blindness, and heart disease, as well as studies of anxiety, depression, PTSD, Alzheimer's disease, Parkinson's disease and other neurological disorders. Confocal microscopy is important to all these studies as it allows fundamental cellular processes to be visualized at high magnification in live and fixed cells of the immune system, brain, heart and other tissues. Specifically, research groups and projects for which the LSM 880 is critical are: 1) Manipulation of apoptosis, pyroptosis and autophagy in macrophages by Francisella tularensis (Dr. Allen). 2) Induction of neutrophil subtype differentiation and DNA release upon Helicobacter pylori infection (Dr. Allen). 3) Studies of microvesicle (ectosome and exosome) release during bacterial infection (Drs. Allen and Nauseef). 4) Studies of retinal ganglion cell death during glaucoma (Dr. Anderson). 5) The role of podosomes in glaucoma and cancer cell motility (Dr. Anderson). 6) Studies of axonal mitochondrial dynamics in glaucoma (Dr. Anderson). 7) Studies of live Staphylococcus aureus biofilms (Dr. Horswill). 8) Analysis of neutrophil trafficking to sites of parasite infection in the skin, and release of parasite exosomes inside macrophages during infection with Leishmania infantum (Dr. Wilson). 9) Studies of neutrophil plasticity in patients with various infectious and inflammatory disorders (Drs. Wilson and Allen). 10) Studies of Glial Maturation Factor abundance and distribution in the brain with respect to pathogenesis of Alzheimer's and Parkinson's disease, and opportunities for therapeutic intervention (Dr. Zaheer). 11) Analyses of neutrophil granules and phagosomes containing S. aureus (Dr. Nauseef). 12) Studies of B-cell TRAF3 using FRET (Dr. Bishop). 13) Localization of normal and mutated acid-sensing ion channels in the brain to elucidate their functions with respect to synaptic plasticity, anxiety disorders, and PTSD (Dr. Wemmie). Thus, acquisition of an LSM 880 will not merely maintain confocal microscopy at the Iowa City VA. Rather, this cutting edge technology will help to ensure that we remain leaders in our respective fields at the forefront of research to improve Veteran's health.

通过此申请，我们向 ORD 共享设备评估计划 (SheEEP) 请求资金，以 购买一台新的共焦显微镜，安装在爱荷华市炎症计划实验室 退伍军人管理局医疗中心。具体来说，我们寻求获得由以下公司制造的 LSM 880 型共焦显微镜： Carl Zeiss, Inc. 此次购买的理由有三个。首先，我们目前的共焦显微镜，蔡司 LSM 510 已有 18 年历史，自 2016 年 9 月起将正式过时。因此，零件和 维护将不再可用，并且该仪器在许多研究中发挥着关键作用 程序，将变得无法使用。其次，我们有机会享受20%的换购优惠 购买 LSM 880 可以享受折扣。第三，也是最重要的一点，LSM 880 有几个特点 这使其处于共焦技术的前沿。因此，购买 LSM 880 将支持尖端技术 研究并将允许开发新的研究项目和科学家之间的新合作 爱荷华市退伍军人管理局研究项目长期以来一直保持着卓越和成就的记录。 我们的研究组合多种多样，包括对多种传染病、炎症和疾病的研究 免疫、青光眼和失明、心脏病，以及焦虑、抑郁、创伤后应激障碍（PTSD）的研究， 阿尔茨海默病、帕金森病和其他神经系统疾病。共焦显微镜很重要 对于所有这些研究，因为它允许在活体和 免疫系统、大脑、心脏和其他组织的固定细胞。具体来说，研究小组和项目 LSM 880 的关键在于： 1) 通过以下方式操纵巨噬细胞中的细胞凋亡、细胞焦亡和自噬 土拉弗朗西斯菌（艾伦博士）。 2) 诱导中性粒细胞亚型分化和 DNA 释放 幽门螺杆菌感染（艾伦博士）。 3) 微泡（胞外体和外泌体）释放过程的研究 细菌感染（Allen 博士和 Nauseef 博士）。 4）青光眼期间视网膜神经节细胞死亡的研究（Dr. 安德森）。 5）足体在青光眼和癌细胞运动中的作用（安德森博士）。 6）研究 青光眼的轴突线粒体动力学（安德森博士）。 7) 活金黄色葡萄球菌生物膜的研究 （霍斯威尔博士）。 8) 分析中性粒细胞向皮肤寄生虫感染部位的运输以及释放 婴儿利什曼原虫感染期间巨噬细胞内的寄生虫外泌体（威尔逊博士）。 9）研究 患有各种感染和炎症性疾病的患者的中性粒细胞可塑性（威尔逊博士和艾伦博士）。 10） 大脑中胶质成熟因子丰度和分布与发病机制的研究 阿尔茨海默病和帕金森病，以及治疗干预的机会（Zaheer 博士）。 11）分析 含有金黄色葡萄球菌的中性粒细胞颗粒和吞噬体（Nauseef 博士）。 12) 使用 B 细胞 TRAF3 研究 FRET（毕夏普博士）。 13）大脑中正常和突变的酸敏感离子通道的定位以阐明 它们在突触可塑性、焦虑症和创伤后应激障碍（PTSD）方面的功能（Wemmie 博士）。 因此，购买 LSM 880 不仅仅可以维持爱荷华市 VA 的共聚焦显微镜。相反，这个 尖端技术将有助于确保我们在各自领域保持领先地位 改善退伍军人健康的研究。