ShEEP Request for Zeiss LSM880 Confocal Microscope

ShEEP 请求蔡司 LSM880 共焦显微镜

• 批准号: 9210692
• 负责人: Lee-Ann H Allen
• 金额: --
• 依托单位: IOWA CITY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9210692
• 关键词: 18 year old; ASIC channel; Achievement; Alzheimer' s Disease; Anxiety; Anxiety Disorders; Apoptosis; Autophagocytosis; B-Lymphocytes; Bacterial Infections; Biological Process; Blindness; Brain; Cell Death; Cell physiology; Cells; Cities; Collaborations; Color; Communicable Diseases; Computer software; Confocal Microscopy; Country; Cytoplasmic Granules; DNA; Data; Data Collection; Detection; Development; Diabetes Mellitus; Disease; Ensure; Equipment; Fluorescence; Fluorescence Resonance Energy Transfer; Francisella tularensis; Funding; Glaucoma; Glia Maturation Factor; Health; Heart; Heart Diseases; Helicobacter Infections; Housing; Image; Image Analysis; Immune system; Immunity; Infection; Inflammation; Inflammatory; Invaded; Iowa; Laboratories; Lasers; Leishmania infantum; Life; Light; Lighting; Maintenance; Medical center; Mental Depression; Microbe; Microbial Biofilms; Microscope; Mitochondria; Modeling; Morphologic artifacts; Movement; Mutate; Noise; Optics; Organelles; Parasites; Parkinson Disease; Pathogenesis; Patients; Phagosomes; Play; Positioning Attribute; Post-Traumatic Stress Disorders; Productivity; Program Evaluation; Proteins; Publications; Request for Applications; Research; Research Project Grants; Resolution; Retinal Ganglion Cells; Role; Running; Sampling; Scanning; Scientist; Series; Signal Transduction; Site; Skin; Speed; Staphylococcus aureus; Support System; Synaptic plasticity; System; TNF receptor-associated factor 3; Technology; Therapeutic Intervention; Thick; Time; Tissue Sample; Tissues; Veterans; Visible Radiation; cancer cell; cell fixing; cell motility; computer monitor; detector; discount; exosome; flexibility; gallium arsenide; improved; insight; instrument; interest; macrophage; microvesicles; nervous system disorder; neutrophil; optical spectra; photomultiplier; programs; quasar; trafficking

With this application we request funds from the ORD Shared Equipment Evaluation Program (SheEEP) to purchase a new confocal microscope to be housed in the Inflammation Program laboratories of the Iowa City VA Medical Center. Specifically, we seek to obtain a model LSM 880 confocal microscope manufactured by Carl Zeiss, Inc. The rationale for this purchase is threefold. First, our current confocal microscope, a Zeiss LSM 510, is 18 years old, and as of September 2016 will be officially obsolete. As such, parts and maintenance will no longer be available, and the instrument, which plays a critical role in many research programs, will become unusable. Second, we have the opportunity to take advantage of a 20% trade-up discount on purchase of the LSM 880. Third and most importantly, there are several features of the LSM 880 that place it at the forefront of confocal technology. Thus, acquisition of an LSM 880 will support cutting edge research and will allow the development of new research projects and new collaborations among scientists of the Iowa City VA research program, which has a long-standing record of excellence and achievement. Our research portfolio is diverse and includes studies of several infectious diseases, inflammation and immunity, glaucoma and blindness, and heart disease, as well as studies of anxiety, depression, PTSD, Alzheimer's disease, Parkinson's disease and other neurological disorders. Confocal microscopy is important to all these studies as it allows fundamental cellular processes to be visualized at high magnification in live and fixed cells of the immune system, brain, heart and other tissues. Specifically, research groups and projects for which the LSM 880 is critical are: 1) Manipulation of apoptosis, pyroptosis and autophagy in macrophages by Francisella tularensis (Dr. Allen). 2) Induction of neutrophil subtype differentiation and DNA release upon Helicobacter pylori infection (Dr. Allen). 3) Studies of microvesicle (ectosome and exosome) release during bacterial infection (Drs. Allen and Nauseef). 4) Studies of retinal ganglion cell death during glaucoma (Dr. Anderson). 5) The role of podosomes in glaucoma and cancer cell motility (Dr. Anderson). 6) Studies of axonal mitochondrial dynamics in glaucoma (Dr. Anderson). 7) Studies of live Staphylococcus aureus biofilms (Dr. Horswill). 8) Analysis of neutrophil trafficking to sites of parasite infection in the skin, and release of parasite exosomes inside macrophages during infection with Leishmania infantum (Dr. Wilson). 9) Studies of neutrophil plasticity in patients with various infectious and inflammatory disorders (Drs. Wilson and Allen). 10) Studies of Glial Maturation Factor abundance and distribution in the brain with respect to pathogenesis of Alzheimer's and Parkinson's disease, and opportunities for therapeutic intervention (Dr. Zaheer). 11) Analyses of neutrophil granules and phagosomes containing S. aureus (Dr. Nauseef). 12) Studies of B-cell TRAF3 using FRET (Dr. Bishop). 13) Localization of normal and mutated acid-sensing ion channels in the brain to elucidate their functions with respect to synaptic plasticity, anxiety disorders, and PTSD (Dr. Wemmie). Thus, acquisition of an LSM 880 will not merely maintain confocal microscopy at the Iowa City VA. Rather, this cutting edge technology will help to ensure that we remain leaders in our respective fields at the forefront of research to improve Veteran's health.

在此申请中，我们要求从ORD共享设备评估计划（SHEEEP）中的资金 购买一个新的共聚焦显微镜，可以容纳在爱荷华城的炎症计划实验室中 VA医疗中心。具体而言，我们寻求获得由LSM 880型号共聚焦显微镜制造的 Carl Zeiss，Inc。此购买的基本原理是三倍。首先，我们当前的共聚焦显微镜，Zeiss LSM 510，18岁，截至2016年9月，将正式过时。因此，零件和 维护将不再可用，并且该工具在许多研究中起着至关重要的作用 程序将变得无法使用。其次，我们有机会利用20％的交易 购买LSM 880的折扣。第三，最重要的是，LSM 880有多个功能 这将其置于共聚焦技术的最前沿。因此，获取LSM 880将支持尖端 研究并将允许开发新的研究项目和新的合作 爱荷华城弗吉尼亚州研究计划，该计划具有卓越和成就的长期记录。 我们的研究组合是多种多样的，包括对几种传染病，炎症和 免疫力，青光眼和失明以及心脏病，以及焦虑，抑郁，PTSD的研究， 阿尔茨海默氏病，帕金森氏病和其他神经系统疾病。共聚焦显微镜很重要 对于所有这些研究 固定免疫系统，大脑，心脏和其他组织的细胞。具体而言，研究小组和项目 LSM 880至关重要的是：1）通过巨噬细胞中凋亡，凋亡和自噬的操纵 弗朗西斯拉·特拉琳斯（Allen）。 2）诱导中性粒细胞亚型分化和DNA释放 幽门螺杆菌感染（艾伦博士）。 3）在研究期间的微囊泡（外胞体和外泌体）释放的研究 细菌感染（Dr.Allen和Nauseef博士）。 4）在青光眼期间进行视网膜神经节细胞死亡的研究（博士 安德森）。 5）足体在青光眼和癌细胞运动中的作用（Anderson博士）。 6）研究 青光眼中的轴突线粒体动力学（Anderson博士）。 7）活体金黄色葡萄球菌生物膜的研究 （Horswill博士）。 8）分析中性粒细胞运输到皮肤中寄生虫感染部位的分析，并释放 巨噬细胞内巨噬细胞内的寄生虫外泌体（威尔逊博士）。 9）研究 患有各种感染性和炎症性疾病的患者的中性粒细胞可塑性（威尔逊和艾伦博士）。 10） 关于大脑中神经胶质成熟因子丰度和大脑分布的研究 阿尔茨海默氏病和帕金森氏病，以及治疗干预的机会（Zaheer博士）。 11）分析 含有金黄色葡萄球菌的中性粒细胞颗粒和吞噬体（Nauseef博士）。 12）使用B细胞TRAF3的研究 Fret（Bishop博士）。 13）脑中正常和突变的酸性离子通道的定位以阐明 它们在突触可塑性，焦虑症和PTSD（Wemmie博士）方面的功能。 因此，对LSM 880的获取不仅会在爱荷华州弗吉尼亚州维护共聚焦显微镜。相反，这个 尖端技术将有助于确保我们在各自领域保持领导者的最前沿 研究以改善退伍军人的健康。