Periodontal Biomaterials with BITE (Biofilm Immunity with Tissue Engineering)

具有 BITE（组织工程生物膜免疫）的牙周生物材料

• 批准号: 9005853
• 负责人: James D. Bryers
• 金额: $ 63.9万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-15 至 2020-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9005853
• 关键词: Affect; Anti-Infective Agents; Antibiotics; Antigen-Presenting Cells; Attention; Bacterial Infections; Behavior; Biocompatible Materials; Biological Assay; Bone Tissue; Cell Count; Cell Differentiation process; Cell Line; Cell Lineage; Cell membrane; Cells; Cephalic; Chimeric Proteins; Construction Materials; Cues; Defect; Dendritic Cells; Engineering; Ethical Issues; Freedom; Galactosidase; Generations; Genes; Goals; Grant; Guided Tissue Regeneration; Healed; Health; Human; Immune response; Immunity; Implant; In Vitro; Infection; Knockout Mice; LacZ Genes; Lead; Limited Stage; Mass Spectrum Analysis; Mediating; Membrane Proteins; Messenger RNA; MicroRNAs; Microbial Biofilms; Mus; Myelogenous; Myeloid Cells; National Institute of Dental and Craniofacial Research; Opsonin; Oral; Particulate; Pathway interactions; Periodontitis; Phagocytosis; Phenotype; Polymers; Population; Population Sizes; Porphyromonas gingivalis; Proteins; Regenerative Medicine; Reporter; Reporter Genes; Research; Research Project Grants; Resort; Rest; Role; Science; Shapes; Signal Transduction; Skeletal Muscle; Stem Cell Research; Stem cells; Study Section; System; Technology; Time; Tissue Engineering; Tissues; Toll-like receptors; Transgenic Mice; United States National Institutes of Health; Vesicle; adaptive immunity; base; exosome; healing; in vitro Assay; in vivo; injured; macrophage; monocyte; nano; novel; oral pathogen; pathogen; prevent; progenitor; promoter; proteomic signature; reconstruction; research study; scaffold; time use; tissue regeneration; transcription factor; transcriptomics; uptake; vector vaccine

 DESCRIPTION (provided by applicant): Our original NIDCR grant (DE018701) developed guided tissue regeneration (GTR) scaffolds for the reconstruction of periodontitis--‐caused infrabony defects that employed a new non--‐antibiotic based concept in biomaterials that, for the first time, promoted both a short--‐ and a long--‐term immune response to a specific oral bacterial pathogen, Porphyromonas gingivalis (PG). Using a novel templating technology, we can fabricate polymer porous templated scaffolds (PTS) where every pore is the same size and pore interconnects are also uniform in size, with both parameters being adjustable. If the pore size is ~35μm, PTS show remarkable healing in numerous soft and hard tissues; independent of the polymer used. Given that our original project created PTS that successfully prevent bacterial infection, we now turn our attention in this renewal to defining the underlying mechanisms governing the tissue regeneration observed only in the 35μm PTS. Our transformative research will first prove and then second develop the "heretical" concept that other myeloid cells, i.e., th macrophage, can be engineered to trans--‐differentiate into desired non--‐myeloid lineages. Considering the population size of macrophage cells within humans, ease of access, and the ethical issues related to stem cell research, directing macrophage differentiation, rather than stem cells, could create whole new science paradigms and regenerative medicine technologies.

 描述（由申请人提供）：我们最初的 NIDCR 拨款 (DE018701) 开发了用于重建牙周炎引起的骨下缺陷的引导组织再生 (GTR) 支架，该支架首次在生物材料中采用了新的非抗生素概念，针对特定口腔细菌病原体牙龈卟啉单胞菌（PG）的短期和长期免疫反应。利用一种新颖的模板技术，我们可以制造聚合物多孔模板支架（PTS），其中每个孔的尺寸相同，孔互连的尺寸也均匀，并且这两个参数都是可调的，如果孔径为~35μm，PTS在30℃下表现出显着的愈合效果。大量的软组织和硬组织；考虑到我们最初的项目创建了成功预防细菌感染的 PTS，我们现在将注意力转向定义仅在 35μm 中观察到的组织再生的基本机制。 PTS。我们的变革性研究将首先证明然后发展“异端”概念，即考虑到细胞群的规模，其他骨髓细胞（即巨噬细胞）可以被改造为所需的非骨髓细胞谱系。人类体内的巨噬细胞、获取的便利性以及与干细胞研究相关的伦理问题，指导巨噬细胞而不是干细胞的分化，可以创造全新的科学范式和再生医学技术。