Metal-titanates as Novel Inhibitors of Cariogenic Biofilms at Tooth-composite Int

金属钛酸盐作为牙齿复合材料国际中心致龋生物膜的新型抑制剂

基本信息

批准号：
8141302
负责人：
James D. Bryers
金额：
$ 36.61万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-10 至 2014-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8141302
关键词：
3-Dimensional Affect Anti-Bacterial Agents Area Bacteria Biological Process Bioreactors Ceramics Characteristics Communities Complex Composite Resins Computer software Data Dental Bonding Dental Enamel Dental caries Dentin Fatigue Filler Funding Goals Growth Human Volunteers In Situ In Vitro Infection Investigation Ion Exchange Ions Knowledge Laboratories Laser Microscopy Lesion Longevity Mechanics Metabolism Metals Microbe Microbial Biofilms Modeling Monoclonal Antibodies Morphology Nature Optics Oral Outcome Particle Size Plant Resins Population Distributions Population Dynamics Process Property Quantum Dots Reporting Reproducibility Research Research Personnel Resource Sharing Structure Surface Techniques Tooth structure United States National Institutes of Health Work absorption antibody conjugate antimicrobial cariogenic bacteria composite restoration human subject improved in vivo inhibitor/antagonist innovation metal complex microbial microleakage multi-photon multidisciplinary nanoparticle novel physical process physical property public health relevance restoration

项目摘要

DESCRIPTION (provided by applicant): Our goal of this investigation is to develop a new class of antibacterial nanoparticle titanate-metal complexes. Micro titanate-metal complexes have been shown to have antimicrobial activity. At present, there are no data on the interactions of nanoparticle titanate with bacteria and on the antibacterial properties of metal ions of Au(III), Pd(II), and Pt(IV). The central hypothesis of this investigation is that nanoparticle titanate-metal complexes that are incorporated into a dental bonding resin will inhibit cariogenic bacterial growth in a biofilm adjacent to the tooth/composite restoration interface without compromising resin-tooth bonding. The proposed work is innovative, because it capitalizes on a new class of titanate-metal complexes as antibacterial agents. In addition, biofilm population dynamics with respect to tooth/composite restoration interface will be quantified non-invasively by Mab-conjugated quantum dots. There are only three such reports on bacterial biofilms and none applied to cariogenic bacteria. The combination of the work proposed is expected to allow the clinicians to tackle the composite longevity problem at the weakest interface. By exploring the interactions between titanate-metal complexes and bacteria biofilm, we will bridge a major gap in knowledge about a new class of titanate-metal complexes antibacterials. PUBLIC HEALTH RELEVANCE: Our investigation will develop a new class of antibacterial nanoparticle titanate-metal complexes of Au(III), Pd(II), and Pt(IV). We will incorporate this titanate-metal complexes into a dental bonding resin, which will inhibit cariogenic bacterial growth in a biofilm adjacent to the tooth/composite restoration interface. We will study the biofilm collected both in the laboratory and from human subjects with multi-photon laser microscopy.

描述（由申请人提供）：我们研究的目标是开发一类新的抗菌纳米颗粒钛酸钛盐 - 金属配合物。微钛酸金属配合物已显示出具有抗菌活性。目前，尚无有关纳米颗粒钛与细菌的相互作用以及Au（III），PD（II）和PT（IV）金属离子的抗菌特性的相互作用。这项研究的中心假设是，纳米颗粒钛酸 - 金属复合物掺入牙科粘结树脂中将抑制与牙齿/复合恢复界面相邻的生物膜中的肉芽生细菌生长，而不会损害树脂牙齿粘合。拟议中的工作具有创新性，因为它将新的泰坦盐 - 金属络合物作为抗菌剂进行了利用。另外，将对牙齿/复合恢复界面相对于牙齿/复合恢复界面的生物膜种群动力学将通过mAb偶联的量子点进行非侵入性。关于细菌生物膜的报告只有三个这样的报告，没有任何报道适用于致癌细菌。预计提议的工作的组合将使临床医生能够在最弱的界面解决复合寿命问题。通过探索泰坦酸 - 金属复合物与细菌生物膜之间的相互作用，我们将弥合有关一类新的泰坦酸 - 金属配合物抗细菌抗菌物质的主要差距。公共卫生相关性：我们的调查将开发一类新的AU（III），PD（II）和PT（IV）的抗菌纳米颗粒钛钛酸钛合金复合物。我们将将这种钛金属络合物掺入牙齿粘合树脂中，该树脂将抑制与牙齿/复合恢复界面相邻的生物膜中的致癌细菌生长。我们将研究在实验室和具有多光子激光显微镜的人类受试者中收集的生物膜。