Metal-titanates as Novel Inhibitors of Cariogenic Biofilms at Tooth-composite Int

金属钛酸盐作为牙齿复合材料国际中心致龋生物膜的新型抑制剂

基本信息

批准号：
8141302
负责人：
James D. Bryers
金额：
$ 36.61万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-10 至 2014-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8141302
关键词：
3-Dimensional Affect Anti-Bacterial Agents Area Bacteria Biological Process Bioreactors Ceramics Characteristics Communities Complex Composite Resins Computer software Data Dental Bonding Dental Enamel Dental caries Dentin Fatigue Filler Funding Goals Growth Human Volunteers In Situ In Vitro Infection Investigation Ion Exchange Ions Knowledge Laboratories Laser Microscopy Lesion Longevity Mechanics Metabolism Metals Microbe Microbial Biofilms Modeling Monoclonal Antibodies Morphology Nature Optics Oral Outcome Particle Size Plant Resins Population Distributions Population Dynamics Process Property Quantum Dots Reporting Reproducibility Research Research Personnel Resource Sharing Structure Surface Techniques Tooth structure United States National Institutes of Health Work absorption antibody conjugate antimicrobial cariogenic bacteria composite restoration human subject improved in vivo inhibitor/antagonist innovation metal complex microbial microleakage multi-photon multidisciplinary nanoparticle novel physical process physical property public health relevance restoration

项目摘要

DESCRIPTION (provided by applicant): Our goal of this investigation is to develop a new class of antibacterial nanoparticle titanate-metal complexes. Micro titanate-metal complexes have been shown to have antimicrobial activity. At present, there are no data on the interactions of nanoparticle titanate with bacteria and on the antibacterial properties of metal ions of Au(III), Pd(II), and Pt(IV). The central hypothesis of this investigation is that nanoparticle titanate-metal complexes that are incorporated into a dental bonding resin will inhibit cariogenic bacterial growth in a biofilm adjacent to the tooth/composite restoration interface without compromising resin-tooth bonding. The proposed work is innovative, because it capitalizes on a new class of titanate-metal complexes as antibacterial agents. In addition, biofilm population dynamics with respect to tooth/composite restoration interface will be quantified non-invasively by Mab-conjugated quantum dots. There are only three such reports on bacterial biofilms and none applied to cariogenic bacteria. The combination of the work proposed is expected to allow the clinicians to tackle the composite longevity problem at the weakest interface. By exploring the interactions between titanate-metal complexes and bacteria biofilm, we will bridge a major gap in knowledge about a new class of titanate-metal complexes antibacterials. PUBLIC HEALTH RELEVANCE: Our investigation will develop a new class of antibacterial nanoparticle titanate-metal complexes of Au(III), Pd(II), and Pt(IV). We will incorporate this titanate-metal complexes into a dental bonding resin, which will inhibit cariogenic bacterial growth in a biofilm adjacent to the tooth/composite restoration interface. We will study the biofilm collected both in the laboratory and from human subjects with multi-photon laser microscopy.

描述（由申请人提供）：我们这项研究的目标是开发一类新型抗菌纳米颗粒钛酸盐-金属复合物。微钛酸盐-金属络合物已被证明具有抗菌活性。目前，尚无纳米钛酸盐颗粒与细菌相互作用以及Au(III)、Pd(II)和Pt(IV)金属离子抗菌性能的数据。这项研究的中心假设是，掺入牙科粘合树脂中的纳米颗粒钛酸盐-金属复合物将抑制邻近牙齿/复合材料修复体界面的生物膜中致龋细菌的生长，而不会影响树脂-牙齿粘合。这项工作具有创新性，因为它利用了一类新型钛酸盐金属络合物作为抗菌剂。此外，牙齿/复合材料修复界面的生物膜群体动态将通过单克隆抗体共轭量子点进行非侵入性量化。关于细菌生物膜的此类报告只有三篇，没有一篇适用于致龋菌。所提出的工作组合预计将使临床医生能够解决最薄弱界面的复合寿命问题。通过探索钛酸盐-金属络合物和细菌生物膜之间的相互作用，我们将弥合关于新型钛酸盐-金属络合物抗菌剂的知识空白。公共健康相关性：我们的研究将开发一类新型抗菌纳米颗粒钛酸盐金属复合物 Au(III)、Pd(II) 和 Pt(IV)。我们将这种钛酸盐-金属复合物纳入牙科粘合树脂中，这将抑制牙齿/复合材料修复体界面附近生物膜中致龋细菌的生长。我们将使用多光子激光显微镜研究在实验室和人类受试者身上收集的生物膜。