Novel Sample Introduction Methodologies for Molecular Rotational Resonance (MRR) Spectroscopy

分子旋转共振 (MRR) 光谱的新样品引入方法

• 批准号: 10603098
• 负责人: Voislav Blagojevic
• 金额: $ 100.63万
• 依托单位: BRIGHTSPEC, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-15 至 2025-03-14
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10603098
• 关键词: Acceleration; Achievement; Acoustics; Adoption; Affect; Alanine; Amino Acids; Amino Sugars; Applications Grants; Area; Award; Basic Science; Biological; Biological Monitoring; Collaborations; Collection; Complex Mixtures; Coupling; Cysteine; Detection; Development; Dipeptides; Environmental Monitoring; Environmental Pollutants; Evaluation; Feedback; Generations; Grant; Heart; Histidine; Hour; Individual; Isomerism; Laboratory Research; Laser vaporization; Lasers; Learning; Legal patent; Letters; Marketing; Mass Spectrum Analysis; Measurement; Measures; Methodology; Methods; Molecular; Molecular Structure; Natural Products; Nebulizer; Nuclear Magnetic Resonance; Paper; Peer Review; Peptides; Pharmaceutical Preparations; Pharmacologic Substance; Phase; Preparation; Process; Proline; Research; Research Personnel; Risk; Sampling; Small Business Innovation Research Grant; Solid; Solvents; Specificity; Spectrum Analysis; Speed; Structure; System; Techniques; Technology; Temperature; Testing; Thick; Time; Toxin; Ultrasonics; United States National Institutes of Health; Universities; Validation; Vial device; Volatilization; Work; biological systems; commercial launch; commercialization; cost; design; drug discovery; improved; innovation; insight; instrument; manufacture; metabolomics; method development; novel; operation; prospective; prototype; research and development; screening; small molecule; software development; software systems; sugar; vaporization

BrightSpec Inc. NIH SBIR Application Project Summary Novel Sample Introduction Methodologies for Molecular Rotational Resonance (MRR) Spectroscopy NIH SBIR Direct to Phase II BrightSpec, Inc. Project Summary This project proposes to develop a new analyte sampling technique for Molecular Rotational Resonance (MRR) spectrometers for the volatilization of solid phase analytes. The proposed system, Laser Induced Acoustic Desorption (LIAD), will enable a large number of important compounds to be accessible by MRR, enhancing our understanding in several important areas of research and accelerating pharmaceutical development. The resulting expansion of sampling range and corresponding utility of this valuable technique will greatly accelerate the adoption of MRR in important areas of research and development. MRR is a spectroscopic technique for unambiguous structure determination and quantitation of small molecules, which has been used for decades in academic research laboratories, but only recently commercialized after the recent achievement of several groundbreaking innovations. MRR excels in structural and isomeric analysis, providing a detail of information like nuclear magnetic resonance (NMR), but at a much lower cost and faster speed, with reduced method development. Through working on a final piece of the puzzle, the development of new sampling techniques, BrightSpec seeks to unlock the full analytical potential of this powerful analytical technique to broaden its use throughout a range of applications, including metabolomics, drug discovery, natural product analysis, and the monitoring of environmental pollutants. BrightSpec has evaluated several sampling techniques for volatilization of solid samples and Laser Induced Acoustic Desorption (LIAD) was determined to be uniquely suitable for incorporation with MRR, as the sensitivity of results with these instruments can be adversely affected by the presence of solvents. BrightSpec has evaluated several sampling techniques for volatilization of solid samples and Laser Induced Acoustic Desorption (LIAD was determined to be uniquely suitable for combination with MRR, as the sensitivity of results with these instruments can be adversely affected by the presence of solvents. The project would be conducted by BrightSpec in collaboration with Kenttämaa Labs at Purdue University, which has been developing the LIAD technique for use with mass spectrometry for over 20 years and holds several LIAD-related patents. A proof-of-concept prototype LIAD-MRR instrument has been used to demonstrate the viability of coupling the two techniques. This preliminary work has de-risked this proposed Phase II project, which involves refinement of the prototype to develop a product with the necessary sensitivity, speed, and ease-of-use to facilitate commercial adoption. The resulting product will then be tested thoroughly on relevant samples in market areas where LIAD-MRR is expected to have the greatest impact. Several prospective customer use cases and samples have already been identified. Following this extensive evaluation, the product design will be refined for commercial launch at or before the end of the grant period.

BrightSpec Inc. NIH SBIR 应用 项目概要 分子旋转共振 (MRR) 光谱的新样品引入方法 NIH SBIR 直接进入第二阶段 BrightSpec 公司 项目概要 该项目提议开发一种新的分子旋转分析物采样技术 用于固相分析物挥发的共振 (MRR) 光谱仪。 激光诱导声解吸 (LIAD) 系统将使大量重要的 化合物可通过 MRR 获得，增强我们对几个重要领域的理解 研究并加速药物开发由此产生的样本扩大。 这种有价值的技术的范围和相应的效用将大大加速 重要研究和开发领域的 MRR。 MRR 是一种光谱技术，用于明确结构测定和定量 小分子，在学术研究实验室中已经使用了数十年，但仅 在最近取得了几项突破性创新成果后，最近实现了商业化。 MRR 擅长结构和异构体分析，提供核等详细信息 磁共振 (NMR)，但成本低得多，速度更快，方法更精简 通过解决最后一块难题，开发新的样品。 技术，BrightSpec 寻求释放这种强大的分析技术的全部分析潜力 技术以扩大其在一系列应用中的用途，包括代谢组学、药物 发现、天然产物分析和环境污染物监测。 BrightSpec 评估了几种用于固体样品挥发的采样技术和激光 诱导声解吸 (LIAD) 被确定为特别适合掺入 使用 MRR，因为这些仪器结果的灵敏度可能会受到 BrightSpec 评估了几种溶剂挥发的采样技术。 固体样品和激光诱导声解吸（LIAD 被确定为独特的 适合与 MRR 结合使用，因为这些仪器的结果灵敏度可以 受到溶剂存在的不利影响 该项目将由 BrightSpec 在 与普渡大学 Kenttämaa 实验室合作，该实验室一直在开发 LIAD 技术与质谱的使用已有 20 多年的历史，并拥有多项与 LIAD 相关的技术 专利。 概念验证原型 LIAD-MRR 仪器已用于证明可行性 这项初步工作降低了拟议的第二阶段项目的风险， 其中涉及改进原型以开发具有必要灵敏度的产品， 然后将测试最终产品的速度和易用性，以促进商业采用。 彻底研究 LIAD-MRR 预计拥有最大市场领域的相关样本 已经确定了几个潜在客户用例和样本。 经过广泛的评估后，产品设计将得到完善，以便在 或在资助期结束之前。