Olfactory Mechanisms Supporting Attraction to e-Cigarette Odors

支持电子烟气味吸引的嗅觉机制

基本信息

批准号：
10598027
负责人：
Natalie Lewis Johnson
金额：
$ 4.77万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-01 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10598027
关键词：
Adolescent Basal Ganglia Behavior Binding Binge Eating Brain Cause of Death Computing Methodologies Corpus striatum structure Coupled Cues Data Decision Making Desire for food Dopamine Electronic cigarette Electrophysiology (science)Environment Epidemic Ethology Food Food Selections Frequencies Fruit Goals Human Investigation Maintenance Measures Mediating Methods Midbrain structure Mission Monitor Motivation Motor Mus National Institute on Deafness and Other Communication Disorders National Research Service Awards Neuromodulator Neurons Neurotransmitters Nicotine Nucleus Accumbens Odors Olfactory Pathways Olfactory tubercle Pathway interactions Perception Pharmaceutical Preparations Pharmacology Phase Physiological Positioning Attribute Process Proxy Research Respiration Rewards Rodent Role Sensory Shapes Site Smell Perception Social Behavior Social Interaction Stimulus Strawberries Structure System Teenagers Testing Training Tubular formation United States Ventral Striatum Ventral Tegmental Area Volatilization Work behavior influence cigarette smoking combustible cigarette craving dopaminergic neuron electronic cigarette use electronic cigarette user electronic liquid experience experimental study hedonic in vivo indexing insight multidisciplinary nicotine use olfactory bulb optogenetics psychologic receptor reinforcer respiratory response sex vaping

项目摘要

PROJECT SUMMARY Sensory information from the environment guides behavior. In particular, odors are integral to finding food, identifying danger, and orchestrating social behaviors. Furthermore, odors can become salient cues when paired with reinforcers which may subsequently trigger cravings (e.g., compulsive eating, drug seeking, etc). Adolescent use of nicotine containing electronic cigarettes (e-cigarettes) has skyrocketed in recent years. Along with nicotine, e-cigarettes often contain appealing additives, such as fruit smells, which based upon their hedonics promote the acquisition and maintenance of e-cigarette/nicotine use in humans and rodents. Therefore, the odors emitted from e-cigarettes are of particular importance to their sensory perception. However, the brain systems responsible for mediating attraction to e-cigarette odors are unknown. The tubular striatum (TuS, also known as the olfactory tubercle) is an olfactory region that also receives modulatory input from midbrain dopaminergic (DA) neurons to inform decision-making. The TuS' distinct connectivity and dual roles in reward and sensory processing uniquely position it to mediate attraction to e-cigarette odors. The proposed F31 NRSA will use respiratory monitoring, behavior, site-specific pharmacology, optogenetics, and in vivo electrophysiology to test the hypothesis that dopaminergic systems of the TuS mediate attraction to e-cigarette odors in adolescent mice of both sexes. In Aim 1, I will test the hypothesis that DA's actions in the TuS are responsible for e-cigarette odor attraction. In Aim 2, I will test the hypothesis that TuS neurons expressing DA 1 receptors represent e- cigarette odors, and that their respiratory-coupled activity encodes the difference between e-cigarette odors containing strawberry additive (attractive) versus those without (unattractive). This project will reveal olfactory contributions to e-cigarette use and further, will uncover fundamental insights into brain systems involved in mediating odor attraction.

项目概要来自环境的感官信息指导行为。特别是，气味对于寻找食物至关重要，识别危险并协调社会行为。此外，气味在配对时可以成为显着的线索强化物可能随后引发渴望（例如强迫性饮食、寻求药物等）。青少年近年来，含尼古丁电子烟的使用量猛增。连同尼古丁，电子烟通常含有吸引人的添加剂，例如水果气味，这是基于其享乐性促进人类和啮齿动物获取和维持电子烟/尼古丁的使用。因此，电子烟散发的气味对其感官知觉尤为重要。然而，大脑负责调节电子烟气味吸引力的系统尚不清楚。管状纹状体（TuS，也称为嗅结节）是一个嗅觉区域，也接收来自中脑的调节输入多巴胺能（DA）神经元为决策提供信息。 TuS 独特的连接性和双重奖励角色感官处理使其独特地定位于调节对电子烟气味的吸引力。拟议的 F31 NRSA 将使用呼吸监测、行为、位点特异性药理学、光遗传学和体内电生理学检验 TuS 的多巴胺能系统介导青少年对电子烟气味的吸引力的假设两种性别的小鼠。在目标 1 中，我将检验 DA 在 TuS 中的行为对电子烟负有责任的假设气味吸引力。在目标 2 中，我将检验以下假设：表达 DA 1 受体的 TuS 神经元代表 e- 香烟气味，并且它们的呼吸耦合活动编码了电子烟气味之间的差异含有草莓添加剂（有吸引力）与不含草莓添加剂（缺乏吸引力）。这个项目将揭示嗅觉对电子烟使用的贡献，并将进一步揭示对涉及电子烟的大脑系统的基本见解调解气味吸引力。