Multi-Omics Core

多组学核心

基本信息

批准号：
10594340
负责人：
William Russell Renthal
金额：
$ 81.06万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-19 至 2027-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10594340
关键词：
Back Benchmarking Biological Assay Cell Communication Cell Nucleus Cells Chromium Consultations Core Facility DNA Data Development Dissociation Drops Ecosystem Emergency Situation Ensure Freezing Fresh Tissue Gene Expression Genomics Genotype High-Throughput Nucleotide Sequencing Hour Human Individual Infrastructure Institutes Laboratories Learning Libraries Output Pain Pain Clinics Process Protocols documentation Quality Control RNA Reporting Research Research Personnel Resources Sampling Sensory Ganglia Services Technology Time Tissues Training XCL1 gene assay development cell type chronic pain cost cost effective cost effectiveness data standards epigenomics experience human tissue improved innovation member multi-ethnic multidisciplinary multiple omics next generation sequencing pain processing single cell analysis tool transcriptomics whole genome

项目摘要

PROJECT SUMMARY (Multi-omics Core) Single-cell transcriptomics is a central component of the PRECISION Human Pain Network because these powerful new tools provide unprecedented opportunities for understanding the cell types, molecules, and cell- cell interactions that contribute to chronic pain. The Multi-omics Core will be led by Dr. Renthal and centralizes the single-cell transcriptomic, epigenomic, and next-generation sequencing efforts with an experienced team in both the development, optimization, and analysis of single-cell and next-generation sequencing efforts. The centralization of these cutting-edge technologies will ensure that the established protocols can be applied systematically to all samples from the Center. Specifically, the Core will provide single-cell and single-nucleus multi-omic services to characterize pain-related tissues in both Projects 1 and 2. They will then provide both Projects rapid next-generation sequencing services with 1-3 day turnaround times at cost. Finally, the Core will remain at the cutting-edge of the rapidly evolving field of single-cell genomics and continue to bring innovations that improve quality and reduce costs back to the Center. The Core will also coordinate with similar Cores across the U19 PRECISION Human Pain Network to reconcile protocols and best practices.

项目摘要（多组学核心）单细胞转录组学是 PRECISION 人类疼痛网络的核心组成部分，因为这些强大的新工具为了解细胞类型、分子和细胞提供了前所未有的机会导致慢性疼痛的细胞相互作用。多组学核心将由 Renthal 博士领导并集中与经验丰富的团队一起进行单细胞转录组、表观基因组和下一代测序工作单细胞和下一代测序工作的开发、优化和分析。这这些尖端技术的集中将确保既定的协议能够得到应用系统地处理来自中心的所有样本。具体来说，Core将提供单细胞和单核多组学服务来表征项目 1 和 2 中与疼痛相关的组织。然后他们将提供项目快速的下一代测序服务，周转时间为 1-3 天（按成本计算）。最后，核心将保持在快速发展的单细胞基因组学领域的前沿并不断带来创新提高质量并降低成本返回中心。该核心还将与各地类似的核心进行协调 U19 PRECISION Human Pain Network 来协调协议和最佳实践。