MacMillan: Overall; The Center for High-Throughput Functional Annotation of Natural Products (McMillan)

麦克米伦：总体而言；

• 批准号: 9134596
• 负责人: Roger G Linington
• 金额: $ 96.43万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9134596
• 关键词: Address; Bioinformatics; Biological; Botanical dietary supplements; Botanicals; Cells; Chemicals; Classification; Communities; Complex Mixtures; Data; Data Analyses; Data Analytics; Development; Diet; Discrimination; Face; Gene Expression; Generations; Goals; Grant; Health; Human; Image; Individual; Industry; Informatics; Laboratories; Libraries; Maps; Metadata; Methods; Molecular; Molecular Profiling; Molecular Target; Natural Products; Natural Products Chemistry; Output; Phenotype; Process; Protocols documentation; Reporter Genes; Reporting; Research; Resolution; Safety; Signal Pathway; Speed; Technology; Therapeutic; United States; United States National Institutes of Health; Work; base; chemical genetics; innovation; insight; interest; meetings; metabolomics; molecular scale; novel; open source; programs; research and development; response; screening; success; technology development; tool; tool development; web site

 DESCRIPTION (provided by applicant): The Center for High-Throughput Functional Annotation of Natural Products unites the natural products chemistry, biological screening, data analytics and bioinformatic strengths of UTSW and UC Santa Cruz to generate a center for natural products research that can use innovative strategies to study the biological effects of botanicals and natural products. Assigning the mechanism of action to botanicals and natural products (mixtures and pure compounds) is a rate limiting challenge for their use and development as supplements and therapeutics. Botanicals and dietary supplements are a particular challenge, as these are often complex mixtures that can change in the constituents and concentration of individual compounds. To prosecute our goal of the chemical and biological characterization of botanicals and natural products with appropriate speed, breadth and precision, we are employing high innovative orthogonal cell-based high content phenotypic screening approaches to return bioactive molecules of interest together with information about their molecular targets. These platforms, cytological profiling (Roger Linington) and FUSION (John MacMillan and Michael White) have been demonstrated to work with complex mixtures as well as pure compounds. While both of these are high-throughput platforms that provide unique biological insight in their own right, combining the output through novel bioinformatic methods will allow us to probe natural products in greater detail. Our deliverables will be a data and data analytics pipeline that will help define the mechanism(s) of action of botanicals and natural products, tools to study synergistic and additive relationships and cellular/molecular fingerprints of botanicals and natural products. A vital component of this Center is the dissemination of data to the greater scientific community. A number of searchable, data driven website will be created to allow the scientific community access to the primary data.

 描述（由申请人提供）：天然产物高通量功能注释中心结合了 UTSW 和加州大学圣克鲁斯分校的天然产物化学、生物筛选、数据分析和生物信息学优势，建立了一个天然产物研究中心，可以利用创新技术研究植物和天然产品的生物效应的策略 确定植物和天然产品（混合物和纯化合物）的作用机制是其作为补充剂的使用和开发的速率限制挑战。植物药和膳食补充剂是一个特殊的挑战，因为它们通常是复杂的混合物，单个化合物的成分和浓度可能会发生变化，以适当的速度和广度实现植物药和天然产品的化学和生物表征的目标。和精度，我们正在采用高度创新的基于正交细胞的高内涵表型筛选方法来返回感兴趣的生物活性分子及其分子靶标的信息，这些平台、细胞学分析（Roger Linington）和FUSION（约翰·麦克米伦和迈克尔·怀特）已被证明可以处理复杂的混合物以及纯化合物，虽然这两个平台都是高通量平台，本身可以提供独特的生物学见解，但通过新颖的生物信息学方法将输出结合起来将允许。我们更详细地探索天然产品，我们的交付成果将是数据和数据。 分析管道将有助于定义植物和天然产品的作用机制、研究协同和加性关系以及细胞/分子指纹的工具 该中心的一个重要组成部分是向更大的科学界传播数据，将创建一些可搜索的、数据驱动的网站，以便科学界访问原始数据。