Role of diabetes and nitric oxide release duration on analytical performance of in vivo glucose biosensors

糖尿病和一氧化氮释放持续时间对体内葡萄糖生物传感器分析性能的作用

基本信息

批准号：
10263316
负责人：
Mark H Schoenfisch
金额：
$ 60.65万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-12-01 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10263316
关键词：
Architecture Biological Assay Biosensor Blood Glucose Cell Survival Cellular biology Chemicals Clinical Data Dependence Development Devices Diabetes Mellitus Disease Electrospinning FDA approved Family suidae Fiber Foreign Bodies Funding Future Gene Expression Profiling Genetic Glucose Health Histocompatibility Immune response Impaired wound healing Implant In Vitro Individual Insulin Insulin-Dependent Diabetes Mellitus Knowledge Mediating Membrane Modification Molecular Morphology Nitric Oxide Nitric Oxide Donors Performance Phase Polyurethanes Porosity Protocols documentation Quality of life Reaction Reporting Research Role Signal Transduction Silicon Dioxide Sulfhydryl Compounds System Systems Biology Testing Thick Tissues Vascularization Work base biomaterial compatibility capsule design diabetic expiration glucose monitor glucose sensor implantation implanted sensor improved in vivo in vivo evaluation monitoring device next generation non-diabetic particle performance tests porcine model prevent response sensor usability wound healing

项目摘要

PROJECT SUMMARY The objective of this project is to study the analytical performance benefits of nitric oxide (NO)-releasing percutaneously implanted glucose sensors in a diabetic swine model as a function of NO-release duration sensor membrane porosity, and diabetes state (i.e., insulin versus non-insulin dependent). Continuous glucose monitoring (CGM) devices with superior usability (i.e., for immediate use and extended duration) would greatly increase the ability of those afflicted with diabetes to successfully manage their disease. In the prior funding period, we demonstrated in a non-insulin dependent diabetic swine model that NO-releasing sensor membranes both lessen the FBR and facilitate improved analytical sensor performance up to 28 days—the longest duration evaluated. However, CGM devices are intended for insulin-dependent individuals and longer implantation periods would improve device utility/value. The variance in FBR and in vivo sensor performance for insulin- and non-insulin-dependent subjects is currently unknown but likely significant due to more impaired wound healing mechanisms. We hypothesize that the improvements in FBR and sensor performance that we have reported sensor membranes that release NO for 28 days will be extended to at least two months by using porous sensor membranes that release for 60+ days and passively promote healthy re-vascularization in the proximity of the sensor. Through our work, we will study the influence of both NO release and porosity on tissue biocompatibility and sensor performance as a function of diabetes. In this respect, we will generate new knowledge on how diabetes influences the tissue integration and in vivo sensor performance.

项目概要该项目的目标是研究一氧化氮 (NO) 释放的分析性能优势在糖尿病猪模型中经皮植入葡萄糖传感器作为 NO 释放持续时间传感器的函数膜孔隙度和糖尿病状态（即胰岛素依赖型与非胰岛素依赖型）。具有卓越可用性（即立即使用和延长持续时间）的（CGM）设备将大大提高在之前的资助期间，我们评估了糖尿病患者成功控制疾病的能力。在非胰岛素依赖型糖尿病猪模型中证明，释放 NO 的传感器膜既可以减少 FBR 和促进分析传感器性能长达 28 天——最长的评估持续时间。 CGM 设备适用于胰岛素依赖者，较长的植入时间将改善设备的性能胰岛素依赖型和非胰岛素依赖型受试者的 FBR 和体内传感器性能的差异为目前未知，但很可能是由于伤口愈合机制受损所致。 FBR 和传感器性能的改进，我们已报告传感器膜可释放 NO 28 天通过使用可释放 60 多天且被动的多孔传感器膜，将延长至至少两个月通过我们的工作，我们将研究传感器附近的健康血运重建的影响。 NO 释放和孔隙率对组织生物相容性和传感器性能的影响作为糖尿病的函数。尊重，我们将产生关于糖尿病如何影响组织整合和体内传感器的新知识表现。