Exosome / microvesicle regulation of oocyte developmental competence

外泌体/微泡对卵母细胞发育能力的调节

基本信息

批准号：
8986806
负责人：
LANE K. CHRISTENSON
金额：
$ 18.69万
依托单位：
UNIVERSITY OF KANSAS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-12-15 至 2017-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8986806
关键词：
Antral Applications Grants Bees Binding Biological Process Biology CD81 gene Cattle Cell Communication Cells Communication Competence Complex Confocal Microscopy Data Development Drops Embryo Embryonic Development Endocrine Endocytosis Environment Event Fertility Fertilization Fertilization in Vitro Follicular Fluid Generations Graafian Follicles Health Human Knowledge Label Laboratories Lead Life Liquid substance Measures Mediating MicroRNAs Modeling Molecular Nature Oocytes Ovarian Ovarian Follicle Ovulation Physiology Play Preparation Procedures Process Proteins Regulation Research Rodent Role Signal Transduction Source System Testing Time Vesicle Work analytical tool base exosome extracellular vesicles granulosa cell improved infertility treatment insight macromolecule microvesicles novel oocyte maturation paracrine research study uptake

项目摘要

DESCRIPTION (provided by applicant): Ovarian follicles undergo a highly orchestrated recruitment and selection process under both endocrine and paracrine regulatory mechanisms, such that by the completion of the process, one or more follicle-enclosed oocytes are prepared for fertilization. During this developmental window, a rich and highly complex fluid containing numerous proteins, miRNAs, and other macromolecules accumulates within the follicular antrum to effectively isolate and simultaneously nurture the cumulus-oocyte-complex (COC). Recent studies have shown that small extracellular vesicles, which include exosomes and microvesicles, are abundant within antral fluid. These vesicles are a rich source of proteins, microRNAs and other molecules that can be taken-up by follicular cells and thus may transmit messages between varying regions within follicular environment. The mere presence of microvesicles and exosomes, which we hereafter refer to collectively as folliculosomes, within the antral follicle, strongly suggests a novel mechanism of communication within the developing follicle. Despite this, no studies have as yet identified any biological function of these vesiclesin ovarian physiology. Therefore, we propose to use folliculosomes collected from antral follicles before and after the LH surge to (1) determine the effects of folliculosomes on maturation and developmental competence of cumulus-oocyte complexes and (2) identify the LH-regulatory mechanism(s) that mediate the rapid changes in folliculosomes prior to ovulation. The central hypothesis of the proposed research is that folliculosomes play critical roles in COC maturation and subsequent developmental competence of the oocyte.

描述（由申请人提供）：在内分泌和旁分泌调节机制下，卵巢卵泡经过高度精心策划的募集和选择过程，因此，在过程完成后，准备了一个或多个含卵泡的卵母细胞以施肥。在此发育窗口中，富含众多蛋白质，miRNA和其他大分子的丰富而高度复杂的液体在卵泡粪便中积聚，可有效地分离并同时培育卵形素质细胞 - 复合物（COC）。最近的研究表明，在鼻液中，小的细胞外囊泡（包括外泌体和微泡）很丰富。这些囊泡是可以被卵泡细胞吸收的蛋白质，microRNA和其他分子的丰富来源，因此可能会在卵泡环境中变化区域之间传播消息。在鼻卵泡中，仅存在微囊泡和外泌体（我们之后我们将其共同称为卵泡体），强烈暗示了发育中的卵泡中一种新型的通信机制。尽管如此，尚未确定这些卵巢生理学中这些囊泡的任何生物学功能。因此，我们建议使用从LH激增之前和之后收集的卵泡体，至（1）确定卵泡体对库卢斯 - 元素络合物的成熟和发育能力的影响，（2）确定LH调节机制，确定LH调节机制，介导了folliculicusemes的快速变化。拟议研究的中心假设是卵泡体在COC成熟和随后的卵母细胞发育能力中起关键作用。