KANSAS U COBRE: PREIMPLANTATION RELEASED PROTEINS EMBRYO QUALITY PREDICTORS

堪萨斯大学 COBRE：植入前释放的蛋白质胚胎质量预测因子

• 批准号: 8167981
• 负责人: LANE K. CHRISTENSON
• 金额: $ 22万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167981
• 关键词: Assisted Reproductive Technology; Biochemical Process; Biological Assay; Child; Competence; Computer Retrieval of Information on Scientific Projects Database; Conditioned Culture Media; Development; Embryo; Embryo Transfer; Evaluation; Event; Exhibits; Funding; Goals; Grant; Human; In Vitro; Institution; Kansas; Link; Low Birth Weight Infant; Molecular; Morbidity - disease rate; Mus; Pattern; Pregnancy; Proteins; Research; Research Personnel; Resources; Selection Criteria; Source; System; Therapeutic; Time; United States National Institutes of Health; base; embryonic protein; implantation; in vivo; preimplantation; stem; success; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Presently, ~1% of children born each year in the USA are conceived via assisted reproductive technologies (ART) and world-wide more than 1 million children have been conceived and delivered over the last 2 decades. Children conceived through ART are more likely to exhibit low birth weights and to be delivered prematurely. Much of the morbidity that stems from ART is due to the high rate of multiple gestations associated with this treatment. To date, embryo quality assessments are based on a subjective morphological evaluation of the embryo following in vitro culture. Objective criteria for selection of high quality embryos should increase ART success and make single embryo transfer a more viable therapeutic option. Thus, there is a compelling case for the development of analytical and non-invasive assays that predict embryo quality. The long-range goal of this research is to link molecular, cellular and biochemical processes occurring in the early embryo prior to implantation to developmental events that ultimately result in a successful pregnancy. The hypothesis of the proposed research is that the early embryo secretes/releases proteins that reflect the developmental competence of that embryo. This proposal will identify en masse for the first time early embryonic secreted/released protein markers in conditioned medium to begin to establish a signature/pattern of proteins indicative of embryo quality. Aim 1 will identify secreted/released protein(s) from murine embryos exhibiting qualitative differences using tandem mass spectrometry. Aim 2 will validate whether these proteins can be used to predict in vivo embryo quality and whether the murine system is translatable to the human system.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 目前，在过去的20年中，通过辅助生殖技术（ART）构思和分娩了约1％的儿童，是通过辅助生殖技术（ART）和全球超过100万儿童构想的。通过艺术构想的孩子更有可能表现出低出生体重并过早地分娩。源于艺术的大部分发病率是由于与这种治疗相关的多种妊娠率高。迄今为止，胚胎质量评估基于对体外培养后胚胎的主观形态学评估。 选择高质量胚胎的客观标准应提高艺术成功，并使单个胚胎转移成为更可行的治疗选择。 因此，有一个令人信服的案例，用于开发预测胚胎质量的分析和非侵入性测定法。这项研究的远距离目标是将早期胚胎中发生的分子，细胞和生化过程联系起来，然后才植入发育事件，最终导致成功怀孕。拟议研究的假设是，早期的胚胎分泌/释放蛋白质反映了该胚胎的发育能力。 该提案将首次在条件培养基中首次确定早期胚胎分泌/释放的蛋白质标记物，以开始建立指示胚胎质量的蛋白质的签名/模式。 AIM 1将使用串联质谱法鉴定出表现出定性差异的鼠胚胎的分泌/释放蛋白。 AIM 2将验证这些蛋白质是否可以用于预测体内胚胎质量以及鼠系统是否可以转换为人类系统。