Identifying Differential Psychosocial and Neurobiological Risk Factors of the Transition from Acute to Chronic Pain in Black and Non-­Hispanic White Adults

识别黑人和非西班牙裔白人成人从急性疼痛转变为慢性疼痛的差异心理社会和神经生物学危险因素

• 批准号: 10576262
• 负责人: Bright Eze
• 金额: $ 4.39万
• 依托单位: UNIVERSITY OF CONNECTICUT STORRS
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-02-25 至 2024-02-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10576262
• 关键词: Achievement; Activities of Daily Living; Acute; Address; Adult; Affect; Age; Area; Biological; Black American; Black Populations; Black race; Blood specimen; CREB1 gene; Chronic; Chronic low back pain; Clinical Research; Cohort Studies; DNA Methylation; DNA methylation profiling; Data; Disparity; Ethnic Origin; Event; Exclusion; Exposure to; Female; Foundations; Freezing; Future; Gene Expression; Gene Expression Profile; Genes; Genetic Transcription; Genomics; Goals; Health Sciences; High Prevalence; Incidence; Individual; Inflammation; Inflammation Mediators; Inflammatory; Interleukin-1; Interleukin-6; Intervention; Job Satisfaction; Knowledge; Laboratories; Life; Low Back Pain; Maintenance; Measures; Mentorship; Methods; Moods; Multiomic Data; Musculoskeletal; NF-kappa B; Neurobiology; Not Hispanic or Latino; Pain; Pain Research; Pain Threshold; Pain management; Participant; Pathway interactions; Persons; Phenotype; Physiological; Population; Postdoctoral Fellow; Process; Productivity; Public Health; Quality of life; RNA methylation; Race; Random Allocation; Rehabilitation therapy; Reporting; Research; Risk; Risk Factors; Role; Sampling; Sensory; Severities; Signal Pathway; Site; Socioeconomic Status; Statistical Data Interpretation; Stress; TNF gene; Techniques; Technology; Testing; Time; Traineeship; Training; Tube; United States; Whole Blood; Woman; Work; affective disturbance; age effect; biopsychosocial; black men; black women; career; chronic pain; design; differential expression; disability; ethnic minority population; follow-up; gene environment interaction; health disparity; improved; knowledge integration; low socioeconomic status; mRNA Expression; male; men; neurobiological mechanism; next generation; pain chronification; pain reduction; pain self-management; pain sensitivity; perceived discrimination; perceived stress; preservation; pressure; programs; psychosocial; racial minority population; response; secondary analysis; sex; skills; targeted treatment; theories

Abstract Low back pain affects an estimated 65 million individuals in the United States, and Black Americans are disproportionately affected by the detrimental consequences of pain, including a higher incidence of converting to chronic pain, reduced functional capacity, mood, and quality of life compared to Non-Hispanic whites (NHWs). Empirical evidence regarding the Conserved Transcriptional Response to Adversity (CTRA) suggests that adverse psychosocial conditions, such as perceived stress due to experienced discrimination or low socioeconomic status, cause increased expression of genes involved in inflammation and stress-related pathways. While these pathways theoretically overlap with those that influence the transition from acute to chronic pain, the CTRA has never been systematically examined in Black individuals who are at risk of a chronic pain trajectory. This F31 application was designed to provide the applicant with the knowledge and skills to establish a research trajectory in pain-related health disparities. He has garnered the support of a highly engaged and productive mentorship team to fulfill his traineeship goals and accomplish the study aims. The applicant’s overall goals are to: 1) gain a deep understanding of the methods and measures used in pain research; 2) build expertise in health disparities theory and measures; 3) acquire genomics knowledge and principles for carrying out rigorous laboratory techniques with next generation technologies; 4) perform the sequencing pipeline, statistical analysis and interpretation of multi-omic data; and, 5) advance and integrate knowledge of the psychosocial and neurobiological mechanisms of the transition from acute to chronic low back pain in Black Americans. The proposed study is a secondary analysis of a completed inception cohort study (R01NR013932; n=220) that tracked individuals at the onset of acute low back pain and followed them every six weeks for six months. The applicant will randomly select 40 Black and 40 NHW participants (20 men and 20 women each for a total of 80 participants) who developed chronic low back pain. Preserved whole blood samples that were drawn into PAXgene tubes and immediately frozen at -80oC will be processed (baseline acute low back pain and at 6-months/chronic low back pain) for RNA and DNA-methylation (DNAm) sequencing to assess gene x environment interactions. The study aims are to: 1) Identify differences in psychosocial (pain severity and interference, mood, perceived stress, work satisfaction) and neurobiological (quantitative sensory testing) factors; and, 2) Examine differential mRNA expression and DNAm profiles between Black and NHW participants with low back pain at acute onset and at 6-months follow-up. Race x sex comparisons will also be conducted. The proposed study will provide a first-step toward establishing the applicant’s program of research, and will identify unique factors that influence pain-related health disparities in Black individuals that may be used in the future to develop targeted therapy and ethnically and culturally- tailored pain self-management interventions.

抽象的 据估计，美国有 6500 万人患有腰痛，而美国黑人 不成比例地受到疼痛不利后果的影响，包括较高的转变发生率 与非西班牙裔白人相比，慢性疼痛、功能能力、情绪和生活质量下降 （NHW）关于逆境保守转录反应（CTRA）的经验证据表明 不利的心理社会状况，例如由于经历过歧视或低水平而感受到的压力 失业状态，导致与炎症和压力相关的基因表达增加 虽然这些途径理论上与影响从急性到过渡的途径重叠。 慢性疼痛，CTRA 从未在有慢性疼痛风险的黑人中进行过系统检查 此 F31 应用程序旨在为申请人提供知识和知识。 他获得了建立与疼痛相关的健康差异研究轨迹的技能。 高度敬业且富有成效的导师团队，以实现他的培训目标并完成学习目标。 申请人的总体目标是：1）深入了解用于疼痛的方法和措施 研究；2) 积累健康差异理论和测量方面的专业知识；3) 获取基因组学知识； 使用下一代技术执行严格的实验室技术的原则；4) 执行 测序流程、多组学数据的统计分析和解释；5) 推进和整合 从急性到慢性低血压转变的心理社会和神经生物学机制的知识 美国黑人的背痛。 拟议的研究是对已完成的初始队列的二次分析 研究（R01NR013932；n=220）追踪急性腰痛发作时的个体并对其进行跟踪 申请人将在六个月内每六周随机选择 40 名黑人和 40 名 NHW 参与者（20 名男性）。 总共 80 名参与者中各有 20 名女性患有慢性腰痛。 抽取到 PAXgene 管中并立即冷冻在 -80oC 下的血样将被处理 （基线急性腰痛和 6 个月/慢性腰痛）RNA 和 DNA 甲基化 (DNAm) 测序以评估基因 x 环境相互作用。该研究的目的是：1) 识别差异。 社会心理（疼痛严重程度和干扰、情绪、感知压力、工作满意度）和神经生物学 （定量感官测试）因素；以及，2) 检查差异 mRNA 表达和 DNAm 谱 在急性发作时和 6 个月随访时出现腰痛的黑人和 NHW 参与者之间进行比较。 还将进行比较。拟议的研究将为建立这一目标迈出第一步。 申请人的研究计划，并将确定影响疼痛相关健康差异的独特因素 未来可能会被用来开发靶向治疗的黑人个体，并且在种族和文化上- 量身定制的疼痛自我管理干预措施。

项目成果

A feasibility study on yoga's mechanism of action for chronic low back pain: psychological and neurophysiological changes, including global gene expression and DNA methylation, following a yoga intervention for chronic low back pain.

瑜伽治疗慢性腰痛作用机制的可行性研究：瑜伽干预慢性腰痛后的心理和神经生理变化，包括整体基因表达和 DNA 甲基化。

• 发表时间: 2022-07-07
• 作者: Adhikari, Bandita;Starkweather, Angela;Xu, Wanli;Acabchuk, Rebecca L;Ramesh, Divya;Eze, Bright;Yang, Yuxuan;Yang, Gee Su;Walker, Joseph;Laubenbacher, Reinhard;Park, Crystal L
• 通讯作者: Park, Crystal L