Hiring Non-toxic Virus Nanoparticles to Count Cancer Biomarker Molecules

使用无毒病毒纳米颗粒来计数癌症生物标志物分子

基本信息

批准号：
9070724
负责人：
Chuanbin Mao
金额：
$ 19.52万
依托单位：
UNIVERSITY OF OKLAHOMA
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-09-01 至 2018-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9070724
关键词：
Affect Bacteria Bacteriophage T7 Bacteriophages Binding Biological Markers Body Fluids Buffers Cancer Patient Capsid Cataloging Catalogs Clinical Color Complement Complementary DNA Complex DNA Detection Diagnosis Engineering Eye Family Fiber Fluorescence Gene Expression Genetic Engineering Genetic Transcription Goals Gold Health Human Infection Light Magnetic nanoparticles Magnetism Malignant Neoplasms Malignant neoplasm of prostate Methods MicroRNAs Nanotechnology Oligonucleotides Patients Peptides Phage Display Polymerase Chain Reaction Property Proteins RNA Recombinants Reporting Reproducibility Reverse Transcription Risk Sampling Series Serum Sodium Chloride Structure Surface Tail Techniques Testing Time Tissues Tumor Tissue Untranslated RNA Virus Water base cancer biomarkers cancer diagnosis cancer type circulating microRNA cost design improved microRNA biomarkers nanoparticle novel specific biomarkers

项目摘要

A type of biomarkers for prostate cancer, called microRNAs (miRNAs), has a unique level in serum from cancer patients. Hence, detecting them will allow us to diagnose prostate cancer earlier. Currently, this type of biomarkers can only be detected by quantitative real-time polymerase chain reaction. However, this technique does not generate reproducible and reliable results when it is used to detect miRNAs at a low level. To be able to reliably quantify prostate cancer-specific miRNAs at a low level, we propose an ultrasensitive strategy that hires non-toxic virus nanoparticles for probing the miRNAs. This strategy is based on the fact that the virus nanoparticles can be engineered to become capable of emitting three different lights and form complex with nanoparticles for capturing the target miRNAs. It is thus possible to detect multiple target miRNAs in one platform because the virus nanoparticles can emit three different fluorescence lights for detecting three different target miRNAs when three different fluorescent molecules are presented on the surface of the virus nanoparticles. Our objective in this project is to develop the new strategy in order to quantify multiple prostate cancer miRNA biomarkers in serum samples, including three target miRNAs specific for the prostate cancer. We aim to achieve high sensitivity, accuracy, and reproducibility for detecting the prostate cancer biomarkers in serum. This project will develop a new facile method that can precisely detect the prostate cancer biomarkers and thus can be used for early prostate cancer diagnosis.

一种称为microRNA（miRNA）的前列腺癌的生物标志物在癌症患者的血清中具有独特的水平。因此，检测它们将使我们能够较早诊断前列腺癌。目前，这种类型的生物标志物只能通过定量的实时聚合酶链反应来检测。但是，当使用低水平检测miRNA时，该技术不会产生可重现和可靠的结果。为了能够在低水平上可靠地量化前列腺特异性miRNA，我们提出了一种超敏感的策略，该策略雇用了非毒性病毒纳米颗粒来探测miRNA。该策略基于以下事实：病毒纳米颗粒可以设计成能够发射三种不同的光并与纳米颗粒形成复合物以捕获目标miRNA。因此，可以在一个平台中检测多个靶MiRNA，因为当三个不同的荧光分子在病毒纳米颗粒的表面上呈现三个不同的荧光分子时，病毒纳米颗粒可以发出三种不同的荧光灯来检测三个不同的靶miRNA。我们在该项目中的目标是制定新策略，以量化血清样品中多种前列腺癌miRNA生物标志物，包括针对前列腺癌特异的三个靶MiRNA。我们旨在实现高灵敏度，准确性和可重复性，以检测血清中的前列腺癌生物标志物。该项目将开发一种新的便利方法，该方法可以精确检测前列腺癌生物标志物，因此可以用于早期前列腺癌的诊断。

项目成果

期刊论文数量（20）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Ti nanorod arrays with a medium density significantly promote osteogenesis and osteointegration.

DOI：
10.1038/srep19047
发表时间：
2016-01-08
期刊：
Scientific reports
影响因子：
4.6
作者：
Ning C;Wang S;Zhu Y;Zhong M;Lin X;Zhang Y;Tan G;Li M;Yin Z;Yu P;Wang X;Li Y;He T;Chen W;Wang Y;Mao C
通讯作者：
Mao C

Phage as a Genetically Modifiable Supramacromolecule in Chemistry, Materials and Medicine.

DOI：
10.1021/acs.accounts.5b00557
发表时间：
2016-06-21
期刊：
Accounts of chemical research
影响因子：
18.3
作者：
Cao B;Yang M;Mao C
通讯作者：
Mao C

Cross Talk Between Autophagy and Apoptosis Contributes to ZnO Nanoparticle-Induced Human Osteosarcoma Cell Death.

自噬和细胞凋亡之间的相互作用导致氧化锌纳米粒子诱导的人骨肉瘤细胞死亡

DOI：
10.1002/adhm.201800332
发表时间：
2018-09
期刊：
Advanced healthcare materials
影响因子：
10
作者：
He G;Ma Y;Zhu Y;Yong L;Liu X;Wang P;Liang C;Yang C;Zhao Z;Hai B;Pan X;Liu Z;Liu X;Mao C
通讯作者：
Mao C

Bone-Inspired Spatially Specific Piezoelectricity Induces Bone Regeneration.

受骨启发的空间特异性压电诱导骨再生