Hiring Non-toxic Virus Nanoparticles to Count Cancer Biomarker Molecules

使用无毒病毒纳米颗粒来计数癌症生物标志物分子

• 批准号: 8873755
• 负责人: Chuanbin Mao
• 金额: $ 16.36万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8873755
• 关键词: Affect; Bacteria; Bacteriophage T7; Bacteriophages; Binding; Biological Markers; Body Fluids; Buffers; Cancer Patient; Capsid; Cataloging; Catalogs; Clinical; Color; Complement; Complementary DNA; Complex; DNA; Detection; Diagnosis; Engineering; Eye; Family; Fiber; Fluorescence; Gene Expression; Genetic Engineering; Genetic Transcription; Goals; Gold; Human; Infection; Lead; Magnetism; Malignant Neoplasms; Malignant neoplasm of prostate; Matrix Metalloproteinases; Methods; MicroRNAs; Nanotechnology; Oligonucleotides; Patients; Peptides; Phage Display; Polymerase Chain Reaction; Property; Proteins; Public Health; RNA; Recombinants; Reporting; Reproducibility; Research; Reverse Transcription; Risk; Sampling; Series; Serum; Sodium Chloride; Surface; Tail; Techniques; Testing; Text; Time; Tissues; Tumor Tissue; Untranslated RNA; Virus; Water; abstracting; base; cancer diagnosis; cancer type; circulating microRNA; cost; design; improved; nanoparticle; novel; public health relevance

Abstract 1 R21 CA195607-01 Hiring Non-toxic Virus Nanoparticles to Count Cancer Biomarker Molecules Modified Project Summary/Abstract Section Enter the text here that is the new abstract information for your application. This section must be no longer than 30 lines of text. A type of biomarkers for prostate cancer, called microRNAs (miRNAs), has a unique level in serum from cancer patients. Hence, detecting them will allow us to diagnose prostate cancer earlier. Currently, this type of biomarkers can only be detected by quantitative real-time polymerase chain reaction. However, this technique does not generate reproducible and reliable results when it is used to detect miRNAs at a low level. To be able to reliably quantify prostate cancer-specific miRNAs at a low level, we propose an ultrasensitive strategy that hires non-toxic virus nanoparticles for probing the miRNAs. This strategy is based on the fact that the virus nanoparticles can be engineered to become capable of emitting three different lights and form complex with nanoparticles for capturing the target miRNAs. It is thus possible to detect multiple target miRNAs in one platform because the virus nanoparticles can emit three different fluorescence lights for detecting three different target miRNAs when three different fluorescent molecules are presented on the surface of the virus nanoparticles. Our objective in this project is to develop the new strategy in order to quantify multiple prostate cancer miRNA biomarkers in serum samples, including three target miRNAs specific for the prostate cancer. We aim to achieve high sensitivity, accuracy, and reproducibility for detecting the prostate cancer biomarkers in serum. This project will develop a new facile method that can precisely detect the prostate cancer biomarkers and thus can be used for early prostate cancer diagnosis.

抽象的 1 R21 CA195607-01 使用无毒病毒纳米颗粒来计数癌症生物标志物分子 修改后的项目摘要/摘要部分 在此处输入文本，该文本是您的应用程序的新摘要信息。此部分的文本长度不得超过 30 行。 前列腺癌的一种生物标志物称为 microRNA (miRNA)，在癌症患者的血清中具有独特的水平。因此，检测它们将使我们能够更早地诊断前列腺癌。目前，此类生物标志物只能通过定量实时聚合酶链反应来检测。然而，当该技术用于检测低水平的 miRNA 时，无法产生可重复且可靠的结果。为了能够可靠地定量低水平的前列腺癌特异性 miRNA，我们提出了一种超灵敏策略，采用无毒的病毒纳米颗粒来探测 miRNA。该策略基于以下事实：病毒纳米颗粒可以被设计为能够发射三种不同的光，并与纳米颗粒形成复合物以捕获目标 miRNA。由于当病毒纳米颗粒表面存在三​​种不同的荧光分子时，病毒纳米颗粒可以发出三种不同的荧光来检测三种不同的目标miRNA，因此可以在一个平台中检测多个目标miRNA。我们在这个项目中的目标是开发新策略来量化血清样本中的多种前列腺癌 miRNA 生物标志物，包括前列腺癌特异性的三种目标 miRNA。我们的目标是实现血清中前列腺癌生物标志物检测的高灵敏度、准确性和重现性。该项目将开发一种新的简便方法，可以精确检测前列腺癌生物标志物，从而可用于早期前列腺癌诊断。