Hiring Non-toxic Virus Nanoparticles to Count Cancer Biomarker Molecules

使用无毒病毒纳米颗粒来计数癌症生物标志物分子

基本信息

批准号：
8873755
负责人：
Chuanbin Mao
金额：
$ 16.36万
依托单位：
UNIVERSITY OF OKLAHOMA
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-09-01 至 2016-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8873755
关键词：
Affect Bacteria Bacteriophage T7 Bacteriophages Binding Biological Markers Body Fluids Buffers Cancer Patient Capsid Cataloging Catalogs Clinical Color Complement Complementary DNA Complex DNA Detection Diagnosis Engineering Eye Family Fiber Fluorescence Gene Expression Genetic Engineering Genetic Transcription Goals Gold Human Infection Lead Magnetism Malignant Neoplasms Malignant neoplasm of prostate Matrix Metalloproteinases Methods MicroRNAs Nanotechnology Oligonucleotides Patients Peptides Phage Display Polymerase Chain Reaction Property Proteins Public Health RNA Recombinants Reporting Reproducibility Research Reverse Transcription Risk Sampling Series Serum Sodium Chloride Surface Tail Techniques Testing Text Time Tissues Tumor Tissue Untranslated RNA Virus Water abstracting base cancer diagnosis cancer type circulating microRNA cost design improved nanoparticle novel public health relevance

Affect Bacteria Bacteriophage T7 Bacteriophages Binding Biological Markers Body Fluids Buffers Cancer Patient Capsid Cataloging Catalogs Clinical Color Complement Complementary DNA Complex DNA Detection Diagnosis Engineering Eye Family Fiber Fluorescence Gene Expression Genetic Engineering Genetic Transcription Goals Gold Human Infection Lead Magnetism Malignant Neoplasms Malignant neoplasm of prostate Matrix Metalloproteinases Methods MicroRNAs Nanotechnology Oligonucleotides Patients Peptides Phage Display Polymerase Chain Reaction Property Proteins Public Health RNA Recombinants Reporting Reproducibility Research Reverse Transcription Risk Sampling Series Serum Sodium Chloride Surface Tail Techniques Testing Text Time Tissues Tumor Tissue Untranslated RNA Virus Water abstracting base cancer diagnosis cancer type circulating microRNA cost design improved nanoparticle novel public health relevance

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项目摘要

Abstract 1 R21 CA195607-01 Hiring Non-toxic Virus Nanoparticles to Count Cancer Biomarker Molecules Modified Project Summary/Abstract Section Enter the text here that is the new abstract information for your application. This section must be no longer than 30 lines of text. A type of biomarkers for prostate cancer, called microRNAs (miRNAs), has a unique level in serum from cancer patients. Hence, detecting them will allow us to diagnose prostate cancer earlier. Currently, this type of biomarkers can only be detected by quantitative real-time polymerase chain reaction. However, this technique does not generate reproducible and reliable results when it is used to detect miRNAs at a low level. To be able to reliably quantify prostate cancer-specific miRNAs at a low level, we propose an ultrasensitive strategy that hires non-toxic virus nanoparticles for probing the miRNAs. This strategy is based on the fact that the virus nanoparticles can be engineered to become capable of emitting three different lights and form complex with nanoparticles for capturing the target miRNAs. It is thus possible to detect multiple target miRNAs in one platform because the virus nanoparticles can emit three different fluorescence lights for detecting three different target miRNAs when three different fluorescent molecules are presented on the surface of the virus nanoparticles. Our objective in this project is to develop the new strategy in order to quantify multiple prostate cancer miRNA biomarkers in serum samples, including three target miRNAs specific for the prostate cancer. We aim to achieve high sensitivity, accuracy, and reproducibility for detecting the prostate cancer biomarkers in serum. This project will develop a new facile method that can precisely detect the prostate cancer biomarkers and thus can be used for early prostate cancer diagnosis.

抽象的 1 R21 CA195607-01 雇用无毒病毒纳米颗粒来计算癌症生物标志物分子修改的项目摘要/摘要部分在此处输入文本，这是您应用程序的新摘要信息。本节必须不超过30行文本。一种称为microRNA（miRNA）的前列腺癌的生物标志物在癌症患者的血清中具有独特的水平。因此，检测它们将使我们能够较早诊断前列腺癌。目前，这种类型的生物标志物只能通过定量的实时聚合酶链反应来检测。但是，当使用低水平检测miRNA时，该技术不会产生可重现和可靠的结果。为了能够在低水平上可靠地量化前列腺特异性miRNA，我们提出了一种超敏感的策略，该策略雇用了非毒性病毒纳米颗粒来探测miRNA。该策略基于以下事实：病毒纳米颗粒可以设计成能够发射三种不同的光并与纳米颗粒形成复合物以捕获目标miRNA。因此，可以在一个平台中检测多个靶MiRNA，因为当三个不同的荧光分子在病毒纳米颗粒的表面上呈现三个不同的荧光分子时，病毒纳米颗粒可以发出三种不同的荧光灯来检测三个不同的靶miRNA。我们在该项目中的目标是制定新策略，以量化血清样品中多种前列腺癌miRNA生物标志物，包括针对前列腺癌特异的三个靶MiRNA。我们旨在实现高灵敏度，准确性和可重复性，以检测血清中的前列腺癌生物标志物。该项目将开发一种新的便利方法，该方法可以精确检测前列腺癌生物标志物，因此可以用于早期前列腺癌的诊断。