PILATUS3 X 1M X-ray detector

PILATUS3 X 1M X射线探测器

• 批准号: 9074860
• 负责人: William I Weis
• 金额: $ 55.01万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9074860
• 关键词: Biological; Biomedical Research; Catalysis; Communities; Complex; Coupled; Cryoelectron Microscopy; Crystallography; Data; Funding; Growth; Health; Human; International; Measurement; Methods; Molecular Sieve Chromatography; Mutation; Noise; Optics; Photons; Proteins; Reading; Research; Research Personnel; Resolution; Roentgen Rays; Signal Transduction; Silicon; Source; Structure; System; Technology; Time; United States National Institutes of Health; data acquisition; detector; drug development; improved; macromolecular assembly; macromolecule; protein protein interaction; research study; structural biology; synchrotron radiation; time use; tool

 DESCRIPTION (provided by applicant): Biological small angle x-ray scattering and diffraction (SAXS) provides structural and dynamic information from solutions or partially ordered arrays of biomolecules in physiologically relevant conditions, albeit at lower resolution (~7-10 Å or higher compared to that obtainable from crystallography or cryo-electron microscopy. SAXS is not only a powerful tool to study structures of large macromolecular assemblies in solution, but can also be used for time-resolved studies to investigate large conformational changes that occur during enzymatic catalysis, protein- protein interactions, and folding of macromolecules. Such studies are critical for biomedical research, as they provide basic mechanistic information that can be used to understand the effects of deleterious mutations, and can thereby inform drug development that ultimately impacts human health. The application of SAXS methods to problems in structural biology has seen tremendous growth in recent years, reflecting the need within the research community for experimental methods that can give structural and dynamic information on large multi-domain complexes. The explosive growth of SAXS has largely been enabled by technical advances that include high- brightness, low divergence X-ray sources, advances in X-ray optics, and X-ray detectors. As protein solutions scatter X-rays quite weakly, improved X-ray detectors are a critical enabling technology. The silicon pixel array X-ray detectors are ideally suited for SAXS, due to their zero read noise and photon counting technology, which open up new possibilities for SAXS applications in structural biology. This application is a request for a complete detector system for small angle X-ray scattering, consisting of a PILATUS3 X 1M Pixel Array Detector (PAD) manufactured by Dectris, Inc., and associated control, data acquisition, data handling and storage computing hardware. This system will be installed on beam line 4-2 (BL4-2) at the Stanford Synchrotron Radiation Lightsource (SSRL). The PILATUS3 1M PAD offers many advantages over the presently available detectors installed at the beam line including superior signal-to-noise, increased maximum frame rate, and larger dynamic range. The detector is well suited for size-exclusion chromatography coupled SAXS experiments, time-resolved, and high-throughput static SAXS measurements that will be needed to drive the forefront of BL4-2 research in structural biology on challenging biomedically important systems. It will maintain international competitiveness for NIH funded researchers.

 描述（由适用提供）：生物学小角度X射线散射和衍射（SAX）提供了在物理相关条件下从解决方案或部分有序的生物分子阵列中提供的结构和动态信息，尽管在较低的分辨率下（〜7-10Å或更高）（尽管与晶体学或cryo-electron Miroccopy的较大工具相比，都可以从较大的情况下获得。解决方案，但也可以用于时间分辨的研究，以研究在酶促催化过程中发生的大量会议变化，蛋白质 - 蛋白质相互作用和大分子的折叠对于生物医学研究至关重要。近年来，巨大的增长反映了研究界内的实验方法的需求，这些方法可以提供有关大型多域复合物的结构和动态信息。 SAXS的爆炸性增长在很大程度上是通过技术进步来实现的，这些进步包括高亮度，低差异X射线源，X射线光学元件的进步和X射线检测器。由于蛋白质溶液散射X射线较弱，改进的X射线检测器是一项关键的启示技术。硅像素阵列X射线检测器非常适合SAX，这是由于其零读取噪声和光子计数技术，这为结构生物学中的SAXS应用开辟了新的可能性。该应用程序是针对小角度X射线散射的完整检测系统的请求，由Dectris，Inc。制造的PILATUS3 x 1M像素阵列检测器（PAD）以及关联的控制，数据采集，数据处理和存储计算硬件。该系统将安装在斯坦福同步辐射光（SSRL）的4-2（BL4-2）上的光束线（BL4-2）上。 PILATUS3 1M PAD比当前安装的探测器具有许多优势，包括较高的信噪，最大帧速率和更大的动态范围。该检测器非常适合尺寸排斥色谱耦合的SAXS实验，时间分辨和高通量静态SAXS测量，这些测量将需要在挑战生物医学上重要的系统上推动BL4-2结构生物学研究的最前沿。它将为NIH资助的研究人员保持国际竞争力。