The Biomechanics of Intercalation in Germ-Band Elongation

胚带伸长插层的生物力学

基本信息

批准号：
9022065
负责人：
Dinah Loerke
金额：
$ 43.79万
依托单位：
UNIVERSITY OF DENVER (COLORADO SEMINARY)
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-01-15 至 2019-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9022065
关键词：
3-Dimensional Ablation Actins Actomyosin Adhesions Affect Architecture Automobile Driving Back Behavior Biological Assay Biomechanics Cell Adhesion Cell Communication Cell physiology Cell-Cell Adhesion Cells Chemicals Cochlea Communities Computational Technique Computer Simulation Contracts Data Defect Development Drosophila genus Embryonic Development Epithelial Epithelium Event Failure Fluorescence Funding Germ Goals Grant Growth Head Human Image Analysis Imaging Techniques Individual Intercellular Junctions Intervention Knowledge Lasers Lead Life Maps Measurable Measures Mechanics Mediating Minor Modeling Molecular Molecular Genetics Monte Carlo Method Morphology Myosin ATPase Nature Organ Outcome Palate Process Process Measure Protein Dynamics Proteins Regulation Research Resolution Role Series Shapes Slide Space Perception Specific qualifier value System Tail Time Tissues Toy Variant analytical method base biophysical techniques cell assembly convergent extension genetic approach imaging modality in vivo insight intercalation interest movie novel physical property programs public health relevance quantitative imaging research study tissue reconstruction tool

项目摘要

DESCRIPTION (provided by applicant): One of the primary tissue shaping mechanisms that is utilized during development is tissue elongation, which is essential to the formation of the elongated head-to-tail body axis and to the development of many internal organs. In epithelial sheets, cellular processes that drive local changes in cell-cell adhesion can be harnessed by developmental programs to effect macroscopic changes in tissue architecture such as elongation; one example is the systematic remodeling of the germ-band epithelium in drosophila. The prevailing model is that a system of planar polarity of junction-associated proteins drives the necessary symmetry-breaking of junction remodeling; specifically, it has been proposed that line tension generated by actomyosin contraction drives the shrinking and loss of cell-cell interfaces with specific spatial orientation. To develop a more detailed biomechanical characterization of this process, we are measuring the dynamics of junction contraction and growth with quantitative imaging and analytical methods; based on our preliminary results, we hypothesize that junctions remodel not through tension contraction, but through `sliding' type displacement. Since cell topologies and protein dynamics are rapidly changing during tissue elongation, we are developing novel computational approaches to permit the analysis of remodeling behaviors from the level of junction vertices to cells and whole tissue. These should be analytic tools of broad interest to the scientific community. The approaches used in these studies will draw on advanced imaging, computational and biophysical techniques that are just beginning to be applied in Drosophila. The funding of this project will be the first major grant for the newly established lab, and will represent an important step towards the founding of independent research programs.

描述（由申请人提供）：在发育过程中使用的主要组织机制之一是组织，以伸长的头到尾轴的形成和许多内部器官的发展。驱动细胞粘附的局部变化的细胞过程可以通过N组织结构（例如延长）来衡量；打破连接的重塑表明，通过收缩和细胞细胞界面构造的线，我们根据我们的初步结果'''''类型显示。科学界的广泛感兴趣的工具。实验室，将代表一个重要的步骤，建立独立研究计划。

项目成果

期刊论文数量（2）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Interface extension is a continuum property suggesting a linkage between AP contractile and DV lengthening processes.

DOI：
10.1091/mbc.e21-07-0352
发表时间：
2022-12-01
期刊：
MOLECULAR BIOLOGY OF THE CELL
影响因子：
3.3
作者：
Vanderleest, Timothy E.;Xie, Yi;Smits, Celia;Blankenship, J. Todd;Loerke, Dinah
通讯作者：
Loerke, Dinah

Viscoelastic voyages - Biophysical perspectives on cell intercalation during Drosophila gastrulation.