The Biomechanics of Intercalation in Germ-Band Elongation

胚带伸长插层的生物力学

基本信息

批准号：
9022065
负责人：
Dinah Loerke
金额：
$ 43.79万
依托单位：
UNIVERSITY OF DENVER (COLORADO SEMINARY)
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-01-15 至 2019-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9022065
关键词：
3-Dimensional Ablation Actins Actomyosin Adhesions Affect Architecture Automobile Driving Back Behavior Biological Assay Biomechanics Cell Adhesion Cell Communication Cell physiology Cell-Cell Adhesion Cells Chemicals Cochlea Communities Computational Technique Computer Simulation Contracts Data Defect Development Drosophila genus Embryonic Development Epithelial Epithelium Event Failure Fluorescence Funding Germ Goals Grant Growth Head Human Image Analysis Imaging Techniques Individual Intercellular Junctions Intervention Knowledge Lasers Lead Life Maps Measurable Measures Mechanics Mediating Minor Modeling Molecular Molecular Genetics Monte Carlo Method Morphology Myosin ATPase Nature Organ Outcome Palate Process Process Measure Protein Dynamics Proteins Regulation Research Resolution Role Series Shapes Slide Space Perception Specific qualifier value System Tail Time Tissues Toy Variant analytical method base biophysical techniques cell assembly convergent extension genetic approach imaging modality in vivo insight intercalation interest movie novel physical property programs public health relevance quantitative imaging research study tissue reconstruction tool

项目摘要

DESCRIPTION (provided by applicant): One of the primary tissue shaping mechanisms that is utilized during development is tissue elongation, which is essential to the formation of the elongated head-to-tail body axis and to the development of many internal organs. In epithelial sheets, cellular processes that drive local changes in cell-cell adhesion can be harnessed by developmental programs to effect macroscopic changes in tissue architecture such as elongation; one example is the systematic remodeling of the germ-band epithelium in drosophila. The prevailing model is that a system of planar polarity of junction-associated proteins drives the necessary symmetry-breaking of junction remodeling; specifically, it has been proposed that line tension generated by actomyosin contraction drives the shrinking and loss of cell-cell interfaces with specific spatial orientation. To develop a more detailed biomechanical characterization of this process, we are measuring the dynamics of junction contraction and growth with quantitative imaging and analytical methods; based on our preliminary results, we hypothesize that junctions remodel not through tension contraction, but through `sliding' type displacement. Since cell topologies and protein dynamics are rapidly changing during tissue elongation, we are developing novel computational approaches to permit the analysis of remodeling behaviors from the level of junction vertices to cells and whole tissue. These should be analytic tools of broad interest to the scientific community. The approaches used in these studies will draw on advanced imaging, computational and biophysical techniques that are just beginning to be applied in Drosophila. The funding of this project will be the first major grant for the newly established lab, and will represent an important step towards the founding of independent research programs.

描述（由应用提供）：在发育过程中使用的主要组织塑形机制之一是组织伸长，这对于形成延长的远光体轴以及许多内部器官的发展至关重要。在上皮床单中，可以通过发育计划来利用驱动细胞粘合剂局部变化的细胞过程，以实现组织结构（例如伸长）的宏观变化。一个例子是果蝇中细菌上皮的系统重塑。盛行的模型是，连接相关蛋白的平面极性系统驱动了连接重塑的必要对称性破坏。具体而言，已经提出，肌动球蛋白收缩产生的线张力驱动了特定空间取向的细胞细胞接口的收缩和损失。为了开发此过程的更详细的生物力学表征，我们正在通过定量成像和分析方法来测量连接收缩和生长的动力学。基于我们的初步结果，我们假设连接点不是通过张力收缩来重塑，而是通过“滑动”类型的位移。由于细胞拓扑和蛋白质动力学在组织伸长过程中正在迅速变化，因此我们正在开发新型的计算方法，以允许分析从连接顶点到细胞和整个组织的重塑行为。这些应该是科学界广泛关注的分析工具。这些研究中使用的方法将利用刚刚开始在果蝇中应用的先进成像，计算和生物物理技术。该项目的资金将是新成立的实验室的第一笔主要赠款，这将代表建立独立研究计划的重要一步。

项目成果

期刊论文数量（2）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Interface extension is a continuum property suggesting a linkage between AP contractile and DV lengthening processes.

DOI：
10.1091/mbc.e21-07-0352
发表时间：
2022-12-01
期刊：
MOLECULAR BIOLOGY OF THE CELL
影响因子：
3.3
作者：
Vanderleest, Timothy E.;Xie, Yi;Smits, Celia;Blankenship, J. Todd;Loerke, Dinah
通讯作者：
Loerke, Dinah

Viscoelastic voyages - Biophysical perspectives on cell intercalation during Drosophila gastrulation.