RNA: Structure, Biophysics and Physiology

RNA：结构、生物物理学和生理学

• 批准号: 9353136
• 负责人: Adrian Ferre-D'Amare
• 金额: $ 115.33万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9353136
• 关键词: Accounting; Alternative Splicing; Amino Acids; Anabolism; Anti-Bacterial Agents; Biological; Biological Models; Biophysics; Boxing; Catalytic RNA; Catheters; Cell Aging; Cell Wall; Cells; Chemicals; Clinical; Complex; Dimensions; Disease; Elbow; Engineering; Future; Gene Expression; Genetic; Genetic Transcription; Gram-Positive Bacteria; Health; Implant; Laboratories; Ligands; Messenger RNA; Methodology; Microbial Biofilms; Molecular; Morbidity - disease rate; Nutritional; Paper; Physiological; Physiology; Procedures; Proteins; Publishing; RNA; Regulator Genes; Ribonucleoproteins; Starvation; Structure; Study Subject; Telomerase; Tissues; Transfer RNA; Translations; Vascular Endothelial Growth Factors; Work; base; interest; mRNA Decay; mortality; nutrition; pathogen; response; small molecule; tumor growth

In the past year, we have made progress in our understanding of the mechanism of action of classes of regulatory RNAs that respond to amino acid starvation and to the bacterial cell wall precursor GlcN6P. Work on the T-box, which derives from the paper our laboratory published in 2013 continues with analysis of two important aspects of the function of this genetic regulator: as a molecular ruler, that identifies its ligand (tRNA) based on linear dimensions, and on its ability to recognize a unique feature of tRNA, namely the elbow. Work also continues on analysis and engineering of the function of the glmS ribozyme-riboswitch, which controls cell wall biosynthesis in Gram-positive bacteria, including many pathogens. We also have made advances in methodology for the analysis of biological RNAs.

在过去的一年里，我们对响应氨基酸饥饿和细菌细胞壁前体 GlcN6P 的调节 RNA 类别的作用机制的理解取得了进展。 T-box 的工作源自我们实验室 2013 年发表的论文，继续分析该遗传调节因子功能的两个重要方面：作为分子标尺，根据线性尺寸识别其配体 (tRNA)，以及其识别 tRNA 独特特征（即肘部）的能力。 还在继续对 glmS 核酶核糖开关的功能进行分析和工程设计，该开关控制革兰氏阳性细菌（包括许多病原体）的细胞壁生物合成。 我们还在生物 RNA 分析方法方面取得了进展。