RNA: Tools for Cell Biology

RNA：细胞生物学工具

• 批准号: 8939924
• 负责人: Adrian Ferre-D'Amare
• 金额: $ 47万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8939924
• 关键词: Cells; Cellular biology; Development; Fluorescence Microscopy; Goals; Green Fluorescent Proteins; Imagery; Knowledge; Laboratories; Life; Property; Proteins; RNA; Reporting; Research; Research Project Grants; Spinach - dietary; Structure; Time; Variant; base; design; fluorophore; improved; in vivo; in vivo imaging; insight; next generation; novel; tool

In the past year, we have made a major breakthrough in the development of RNA-based tools for the in vivo imaging of cellular RNAs using fluorescence microscopy. Our collaborators in the Jaffrey laboratory at Cornell had previously reported the development of a fluorescent RNA module called 'Spinach' but further improvement was hampered by a complete lack of knowledge of its structural and biophysical basis of function. We have now determined the structure of Spinach, revealing a completely novel mode of RNA-fluorophore interaction, and have already employed the structure to both, design a minimized variant ('Baby Spinach') and to tune fluoresce properties.

去年，我们在开发基于 RNA 的工具方面取得了重大突破，用于使用荧光显微镜对细胞 RNA 进行体内成像。 我们在康奈尔大学 Jaffrey 实验室的合作者此前曾报道过一种名为“菠菜”的荧光 RNA 模块的开发，但由于完全缺乏对其结构和生物物理功能基础的了解，进一步的改进受到了阻碍。 我们现在已经确定了菠菜的结构，揭示了一种全新的 RNA-荧光团相互作用模式，并且已经利用该结构设计了最小化变体（“小菠菜”）并调整荧光特性。