RNA: Tools for Cell Biology

RNA：细胞生物学工具

• 批准号: 8939924
• 负责人: Adrian Ferre-D'Amare
• 金额: $ 47万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8939924
• 关键词: Cells; Cellular biology; Development; Fluorescence Microscopy; Goals; Green Fluorescent Proteins; Imagery; Knowledge; Laboratories; Life; Property; Proteins; RNA; Reporting; Research; Research Project Grants; Spinach - dietary; Structure; Time; Variant; base; design; fluorophore; improved; in vivo; in vivo imaging; insight; next generation; novel; tool

In the past year, we have made a major breakthrough in the development of RNA-based tools for the in vivo imaging of cellular RNAs using fluorescence microscopy. Our collaborators in the Jaffrey laboratory at Cornell had previously reported the development of a fluorescent RNA module called 'Spinach' but further improvement was hampered by a complete lack of knowledge of its structural and biophysical basis of function. We have now determined the structure of Spinach, revealing a completely novel mode of RNA-fluorophore interaction, and have already employed the structure to both, design a minimized variant ('Baby Spinach') and to tune fluoresce properties.

在过去的一年中，我们在开发基于RNA的工具方面取得了重大突破，用于使用荧光显微镜进行细胞RNA的体内成像。 我们在康奈尔（Cornell）的Jaffrey实验室中的合作者以前曾报道过一个称为“菠菜”的荧光RNA模块的发展，但由于完全缺乏对其功能的结构和生物物理基础的了解，因此阻碍了进一步的改进。 现在，我们已经确定了菠菜的结构，揭示了一种完全新颖的RNA - 氟化体相互作用的模式，并且已经采用了两者的结构，设计了最小化的变体（“婴儿菠菜”）并调整荧光性能。