Development of Faster, Lower-Cost, High-Sensitivity Immunoassay for Specific Determination of Glucagon Using EAP-derived Monoclonal Antibodies

使用 EAP 衍生的单克隆抗体开发更快、成本更低、高灵敏度的免疫测定法，用于特异性测定胰高血糖素

• 批准号: 9107441
• 负责人: MARK GEISBERG
• 金额: $ 22.01万
• 依托单位: SILVER LAKE RESEARCH CORPORATION
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2018-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9107441
• 关键词: Affinity; Amino Acid Sequence; Antibodies; Antibody Affinity; Antibody Specificity; Binding; Biological Assay; Blood; Blood specimen; Buffers; Cells; Clinical Research; Data Set; Development; Disease; Enteroglucagon; Enzyme-Linked Immunosorbent Assay; Epitopes; Genes; Glucagon; Goals; Health; Hormones; Human; Immunization; Immunoassay; Immunodominant Epitopes; Label; Lead; Measures; Methodology; Monoclonal Antibodies; Pancreas; Peptides; Performance; Phase; Plasma; Process; Production; Radioactive Tracers; Reagent; Recovery; Regulation; Reporting; Research; Research Design; Role; Sampling; Serum; Silver; Specificity; System; Testing; Time; Universities; Validation; Whole Blood; base; blood glucose regulation; clinical practice; cost; experience; glucose metabolism; immunogenicity; improved; instrumentation; novel; peptide hormone; polypeptide; pre-clinical; preclinical study; prototype; research clinical testing; small molecule; type I and type II diabetes

 DESCRIPTION (provided by applicant): Glucagon is a peptide hormone with a central role in the regulation of glucose metabolism, and many disorders, including Type 1 and Type 2 diabetes, are notable for abnormalities in glucagon levels. Both clinical practice and research in this field are hampered by the lack of appropriately specific and sensitive assays for glucagon levels in blood. The goal of this project is to develop and validate improved immunoassays for glucagon determination, using recently made hyperspecific monoclonal antibodies to glucagon. The new capabilities offered by these assays can lead to significant advances in the understanding of glucose homeostasis disorders and, in parallel, enable accurate clinical testing for this crucial hormone.

 描述（通过应用证明）：胰高血糖素是一种肽激素，在葡萄糖代谢的调节中具有中心作用，含有1型和2型糖尿病是由于缺乏适当的特定指定的葡萄糖剂量而引起的。血液中的血糖水平改善了胰高血糖素的测定，使用最近特异性的单克隆抗体对葡萄糖的葡萄糖。