Biosafety Level 3 Core

生物安全3级核心

基本信息

批准号：
8883332
负责人：
DANIEL Garrett KAVANAGH
金额：
$ 10.47万
依托单位：
HARVARD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8883332
关键词：
Acquired Immunodeficiency Syndrome Address Africa Animals Area Asia Biology Boston Caribbean region Cell Separation Cells Certification Collaborations Communicable Diseases Communities Consultations Containment Development Disease Environment Epidemic Equipment Faculty Flow Cytometry Fortune Fostering Foundations Future HIV HIV Infections Home environment Human Image Immunology Infection Institutes Instruction International Intervention Trial Journals Laboratories Lead Leadership Life Microbiology Microscopy Molecular Morbidity - disease rate Mus Pathogenesis Play Population Prevention Procedures Publications Research Research Infrastructure Research Personnel Resources Risk Role Safety Scientist South Africa South America Strategic Planning Talents Time Training Training and Education Universities Voice Voting Work animal facility biocontainment facility biosafety level 3 facility cellular imaging co-infection design experience flexibility global health innovation insight instrument instrumentation meetings member mortality mouse model programs research facility research study square foot symposium tissue culture working group

项目摘要

The intersection of TB and HIV infection is increasing the global burden of both diseases. HIV infection greatly increases the risk of TB converting from latent to active infection, and TB infection increases morbidity and mortality in the HIV+ population. In 2006, the HU CFAR sponsored a national meeting on TBHIV coinfection, which led to the creation of a working group of HU CFAR members focused onTB-HlV research, and identification of a major unmet need: a BSL-3 biocontainment facility that would allow for cell sorting and imaging. When new facilities for the Ragon Institute were being constructed, the HU CFAR Membership successfully petitioned the Phillip T and Susan M Ragon Foundation to include a state of the art BSL-3 laboratory to meet these needs. These recently completed facilities include 2240 square feet of BSL- 3 level space, flow cytometry and cell sorting, animal handling, imaging and additional space to accommodate temporary equipment. With HU CFAR support this facility is now home of a newly created HU CFAR BSL-3 Core. Dr. Daniel Kavanagh serves as the PI, working closely with Dr Sarah Fortune, an internationally recognized expert in studies of TB who serves as Director of the newly-created Ragon TB Program, with close interactions with the HU CFAR TB-HIV Scientific Working Group. Drs. Fortune and Kavanagh jointly supervise a team of fellows, staff scientists, and a facility manager who bring extensive experience in safe and productive of BSL-3-level research. The combined talents of this team, as well as state-of-the-art BSL-3 facilities, are, available to the HU CFAR community. HU CFAR users are offered training and certification to conduct experiments within the facility; alternatively Core staff are available to carry out standard tissue culture and microbiology procedures on behalf of researchers who do not wish to work inside the facility. The proposed HU CFAR Biosafety Level 3 Core is guided by the following specific aims: 1. Provide training and consultation for members of HU CFAR community members who are planning TB related projects 2. Provide access to BSL-3 microbiology, tissue culture, live cell microscopy, live FACS, animal facilities, and special projects.

结核病和艾滋病毒感染的交集正在增加两种疾病的全球负担。艾滋病毒感染大大增加了结核病从潜在感染转化为活性感染的风险，结核病感染增加艾滋病毒+人群的发病率和死亡率。 2006年，HU CFAR赞助了TBHIV的全国会议共同感染，导致建立了一个HU CFAR成员的工作组研究和识别主要未满足需求：BSL-3生物膜维护设施，该设施将允许细胞分类和成像。当建造Ragon Institute的新设施时，Hu CFAR 会员资格成功地向Phillip T和Susan M Ragon基金会请愿，包括一个最先进的状态 BSL-3实验室满足这些需求。这些最近完成的设施包括2240平方英尺的BSL- 3级空间，流式细胞术和细胞分类，动物处理，成像和其他空间容纳临时设备。借助HU CFAR支持，该设施现在是新创建的HU的所在地 CFAR BSL-3核心。丹尼尔·卡瓦纳（Daniel Kavanagh）博士是PI，与Sarah Fortune博士密切合作国际公认的结核病研究专家计划，与HU CFAR TB-HIV科学工作组进行密切相互作用。博士。财富和卡瓦纳（Kavanagh）共同监督一组研究员，工作人员科学家和设施经理具有BSL-3级研究的安全和生产力的经验。该团队的合并才能以及 HU CFAR社区可以使用最先进的BSL-3设施。提供HU CFAR用户培训和认证以在设施内进行实验；另外，核心人员可以使用代表不希望的研究人员进行标准的组织培养和微生物学程序在设施内部工作。提出的HU CFAR生物安全3级核心由以下特定指导目标： 1。为正在计划的HU CFAR社区成员的成员提供培训和咨询与结核病相关的项目 2。提供对BSL-3微生物学，组织培养，活细胞显微镜，活体FACS，动物的访问权限设施和特殊项目。