Biosafety Level 3 Core

生物安全3级核心

• 批准号: 9111797
• 负责人: DANIEL Garrett KAVANAGH
• 金额: $ 10.25万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9111797
• 关键词: Acquired Immunodeficiency Syndrome; Address; Africa; Animals; Area; Asia; Biology; Boston; Caribbean region; Cell Separation; Certification; Collaborations; Communicable Diseases; Communities; Consultations; Containment; Development; Disease; Environment; Epidemic; Equipment; Faculty; Flow Cytometry; Fortune; Fostering; Foundations; Future; HIV; HIV Infections; HIV/TB; Home environment; Human; Image; Immunology; Infection; Institutes; Instruction; International; Intervention Trial; Journals; Laboratories; Lead; Leadership; Life; Microbiology; Microscopy; Molecular; Morbidity - disease rate; Mus; Pathogenesis; Play; Population; Prevention; Procedures; Publications; Research; Research Infrastructure; Research Personnel; Resources; Risk; Role; Safety; Scientist; South Africa; South America; Strategic Planning; Talents; Time; Training; Training and Education; Universities; Voice; Voting; Work; animal facility; biocontainment facility; biosafety level 3 facility; cellular imaging; co-infection; design; experience; flexibility; global health; humanized mouse; innovation; insight; instrument; instrumentation; live cell imaging; live cell microscopy; meetings; member; mortality; mouse model; programs; research facility; research study; square foot; symposium; tissue culture; working group

The intersection of TB and HIV infection is increasing the global burden of both diseases. HIV infection greatly increases the risk of TB converting from latent to active infection, and TB infection increases morbidity and mortality in the HIV+ population. In 2006, the HU CFAR sponsored a national meeting on TBHIV coinfection, which led to the creation of a working group of HU CFAR members focused onTB-HlV research, and identification of a major unmet need: a BSL-3 biocontainment facility that would allow for cell sorting and imaging. When new facilities for the Ragon Institute were being constructed, the HU CFAR Membership successfully petitioned the Phillip T and Susan M Ragon Foundation to include a state of the art BSL-3 laboratory to meet these needs. These recently completed facilities include 2240 square feet of BSL- 3 level space, flow cytometry and cell sorting, animal handling, imaging and additional space to accommodate temporary equipment. With HU CFAR support this facility is now home of a newly created HU CFAR BSL-3 Core. Dr. Daniel Kavanagh serves as the PI, working closely with Dr Sarah Fortune, an internationally recognized expert in studies of TB who serves as Director of the newly-created Ragon TB Program, with close interactions with the HU CFAR TB-HIV Scientific Working Group. Drs. Fortune and Kavanagh jointly supervise a team of fellows, staff scientists, and a facility manager who bring extensive experience in safe and productive of BSL-3-level research. The combined talents of this team, as well as state-of-the-art BSL-3 facilities, are, available to the HU CFAR community. HU CFAR users are offered training and certification to conduct experiments within the facility; alternatively Core staff are available to carry out standard tissue culture and microbiology procedures on behalf of researchers who do not wish to work inside the facility. The proposed HU CFAR Biosafety Level 3 Core is guided by the following specific aims: 1. Provide training and consultation for members of HU CFAR community members who are planning TB related projects 2. Provide access to BSL-3 microbiology, tissue culture, live cell microscopy, live FACS, animal facilities, and special projects.

结核病和艾滋病毒感染的交叉正在增加这两种疾病的全球负担。艾滋病毒感染 大大增加了结核病从潜伏感染转为活动性感染的风险，结核病感染增加 HIV+ 人群的发病率和死亡率。 2006年，HU CFAR主办了全国TBHIV会议 合并感染，这导致成立了一个由 HU CFAR 成员组成的工作组，重点关注结核病艾滋病毒 研究和确定主要未满足的需求：允许细胞进行 BSL-3 生物防护设施 排序和成像。当拉根研究所的新设施正在建造时，HU CFAR 会员成功向 Phillip T 和 Susan M Ragon 基金会请愿，纳入最先进的技术 BSL-3实验室可以满足这些需求。这些最近竣工的设施包括 2240 平方英尺的 BSL- 3 层空间、流式细胞术和细胞分选、动物处理、成像和额外空间 容纳临时设备。在 HU CFAR 的支持下，该设施现已成为新创建的 HU 的所在地 CFAR BSL-3 核心。 Daniel Kavanagh 博士担任 PI，与 Sarah Fortune 博士密切合作。 国际公认的结核病研究专家，担任新成立的 Ragon TB 主任 计划，与 HU CFAR TB-HIV 科学工作组密切互动。博士。财富与 卡瓦纳共同管理一个由研究员、科学家和设施经理组成的团队，他们带来了广泛的研究成果。 具有安全、高效的 BSL-3 级研究经验。这个团队的综合人才，以及 HU CFAR 社区可以使用最先进的 BSL-3 设施。为HU CFAR用户提供 在设施内进行实验的培训和认证；或者核心员工可以 代表不希望的研究人员执行标准组织培养和微生物学程序 在设施内工作。拟议的 HU CFAR 生物安全 3 级核心由以下具体指导 目标： 1.为正在规划的HU CFAR社区成员提供培训和咨询 结核病相关项目 2. 提供 BSL-3 微生物学、组织培养、活细胞显微镜、活 FACS、动物 设施和特殊项目。