Multimetallic Catalysis in Biology and Synthesis

生物学和合成中的多金属催化

• 批准号: 10580465
• 负责人: Neal P Mankad
• 金额: $ 0.95万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10580465
• 关键词: Active Sites; Address; Administrative Supplement; Aerobic; Aging; Atmosphere; Award; Biology; Biomimetics; Carbon monoxide dehydrogenase; Case Study; Catalysis; Complement; Complex; Computer software; Core Facility; Equipment; Failure; Fiber Optics; Funding; Goals; Grant; In Situ; Infrastructure; Measurement; Methodology; Modeling; Monitor; Oceans; Organic Synthesis; Parents; Pathway interactions; Reaction; Research; Sampling; Security; Site; catalyst; insight; instrument; metalloenzyme; nitrous oxide reductase; tool

PROJECT SUMMARY This administrative supplement request is proposed to fund procurement of a miniature fiber-optic UV/visible/near-IR spectrometer with accompanying software and sampling accessories. This equipment will significantly accelerate research in the parent award, whose goals are to advance multimetallic catalysis by addressing open scientific questions and challenges regarding (a) biomimetic model complexes inspired by metalloenzymes, and (b) abiological catalysts for organic synthesis methodology. A majority of the research plan during the current grant cycle focused on topic (a), which is the one that will be aided by the proposed procurement of an Ocean-HDX-XR spectrometer with accompanying sampling tools from Ocean Insights. This instrument will complement existing core facilities at the research site, which offer a Windows-95 era UV/visible spectrometer with no near-IR capabilities. Not only will the Ocean-HDX-XR provide security against catastrophic failure of this aging instrument and expand the spectral window in the near-IR region, but it will also facilitate measurements under inert atmosphere by being transferable into gloveboxes using Wifi connectivity. The addition of a dip probe accessory will also facilitate in-situ reaction monitoring and characterization of reactive intermediates. Case studies are presented that highlight the potential benefits of these added capabilities to understand reaction pathways of the CuZ site of nitrous oxide reductase, the Mo/Cu active site of aerobic carbon monoxide dehydrogenase, and other metalloenzymes being studied under the parent award.

项目概要 提出这项行政补充请求是为了资助采购微型光纤 紫外/可见/近红外光谱仪，配有随附的软件和采样附件。该设备 将显着加速母奖的研究，其目标是推进多金属 通过解决有关 (a) 仿生模型的开放科学问题和挑战来发挥催化作用 受金属酶启发的复合物，以及（b）用于有机合成方法的非生物催化剂。 当前资助周期内的大部分研究计划都集中在主题 (a)，该主题 将得到拟议采购的 Ocean-HDX-XR 光谱仪及其随附的帮助 来自 Ocean Insights 的采样工具。该工具将补充现有的核心设施 研究站点，提供 Windows-95 时代的紫外/可见光谱仪，不具备近红外功能。不仅 Ocean-HDX-XR 能否提供安全保障，防止这种老化仪器发生灾难性故障并扩展 近红外区域的光谱窗口，但也有利于惰性气氛下的测量 通过使用 Wifi 连接可转移到手套箱中。添加浸入式探头附件将 还有助于反应中间体的原位反应监测和表征。案例研究是 强调了这些附加功能在理解反应途径方面的潜在好处 一氧化二氮还原酶的 CuZ 位点、需氧一氧化碳的 Mo/Cu 活性位点 脱氢酶和其他金属酶正在母奖下进行研究。