Diversity supplement for the SC2 parent grant

SC2 家长补助金的多元化补充

• 批准号: 10573805
• 负责人: Miguel P Mendez
• 金额: $ 0.71万
• 依托单位: UNIVERSITY OF PUERTO RICO AT AGUADILLA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10573805
• 关键词: Affect; Appointment; Astrocytes; Buffers; Development Plans; Diabetic mouse; Doctor of Philosophy; Electrophysiology (science); Environment; Epilepsy; Exposure to; Female; Funding; Grant; Hippocampus (Brain); Hyperglycemia; Inflammation Mediators; Knowledge; Laboratories; Mus; Neurons; Parents; Postdoctoral Fellow; Potassium; Property; Publishing; Research; Research Personnel; Seizures; TNF gene; Talents; Training; Type 2 diabetic; Work; base; career development; diabetic; experience; experimental study; insight; interest; male; member; neuroinflammation; non-diabetic; parent grant; potassium ion; recruit

This is a diversity supplement to an SC2 Score grant parent grant entitled: “Hippocampal astrocytic Kir4.1 channel function in Type 2 diabetic mice: impact on neuronal hyperexcitability”. The purpose of this request is to provide supplemental funding to support and enhance the training of Ph.D. candidate, Mr. Luis A. Rojas-Colón. The recruitment of Mr. Luis A. Rojas-Colón will allow our laboratory to incorporate neuroinflammation studies that will substantially add to the parent grant and to the laboratory team. With his neuroinflammation Ph.D. training, he will assess if pro-inflammatory mediators could be affecting the ability of astrocytes to buffer potassium ions in the hippocampus and if those pro-inflammatory mediators affect neuronal epileptiform activity. Having Mr. Luis A. Rojas-Colón in the laboratory for the next 2 years would be fitting because of Luis’ research, talent, and interest in neuroinflammation. Since he would share his knowledge with our laboratory members, he will be directly enhancing the diversity environment in our workroom facilitating the qualification of this appointment for a diversity supplement. Through focused discussions, Dr. Miguel P. Méndez-González (PI of the parent grant) and Mr. Luis A. Rojas-Colón, together, have developed new specific aims that will take advantage of our recent published findings and of the parent grant specific aims. In 2020, we published that diabetic male mice display neuronal hyperexcitability and decreased astrocytic K+ conductance. Also, it has been shown that TNF-a reduces astroglia potassium conductance (Köller et al., 1998), while neuronal exposure to IL-1b results in neuronal seizure like activity (Schäfers & Sorkin, 2008). By using Electrophysiology, they will determine the astrocytic and neuronal electrophysiological properties in diabetic and non-diabetic female (control) mice. Overall, the developed experiments in this supplement will provide insight of additional mechanism of how hyperglycemia contributes to epileptiform activity.

这是 SC2 分数补助金家长补助金的多样性补充，题为：“Hippocampal 2 型糖尿病小鼠星形胶质细胞 Kir4.1 通道功能：对神经过度兴奋的影响”。 该请求的目的是提供补充资金以支持和加强 博士生 Luis A. Rojas-Colón 先生的培训 Luis A. Rojas-Colón 先生的招聘。 将使我们的实验室能够纳入神经炎症研究，这将大大增加 家长资助和实验室团队通过他的神经炎症博士培训，他将评估。 促炎介质是否可能影响星形胶质细胞缓冲钾离子的能力 在海马体中以及那些促炎介质是否影响神经元癫痫样活动。 Luis A. Rojas-Colón 先生未来两年在实验室工作是合适的，因为 路易斯对神经炎症的研究、天赋和兴趣，因为他会分享他的知识。 他将与我们的实验室成员一起直接增强我们的多样性环境 工作场所促进此任命的资格以获得多元化补充。 通过集中讨论，Miguel P. Méndez-González 博士（家长资助项目的 PI）和 Mr. Luis A. Rojas-Colón 共同制定了新的具体目标，这些目标将利用我们的优势 最近发表的研究结果和家长资助的具体目标 2020 年，我们发表了糖尿病患者的研究结果。 雄性小鼠表现出神经过度兴奋和星形细胞 K+ 电导降低。 研究表明，TNF-a 会降低星形胶质细胞的钾电导（Köller 等人，1998），而 神经元暴露于 IL-1b 会导致神经元癫痫样活动（Schäfers & Sorkin，2008）。 使用电生理学，他们将确定星形胶质细胞和神经电生理学 总体而言，所开发的实验在糖尿病和非糖尿病雌性（对照）小鼠中的特性。 在本补充品中将提供有关高血糖如何影响的其他机制的见解 至癫痫样活动。