Comprehensive NeuroAIDS Center

综合神经艾滋病中心

基本信息

批准号：
9144628
负责人：
Kamel Khalili
金额：
$ 173.11万
依托单位：
TEMPLE UNIV OF THE COMMONWEALTH
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-08-05 至 2021-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9144628
关键词：
Acquired Immunodeficiency Syndrome Adenoviruses Affect Aftercare Animal Behavior Animal Model Appointment Area Astrocytes Autopsy Basic Science Biocompatible Materials Bioinformatics Biological Models Biopsy Brain CRISPR/Cas technology Cell Culture Techniques Cell model Cell physiology Cells Central Nervous System Diseases Central Nervous System Infections Cities Clinic Clinical Clinical Investigator Clinical Medicine Clinical Research Collaborations Communicable Diseases Comorbidity DNA Sequence Data Analyses Data Base Management Development Discipline Disease Disease Progression Employment Ensure Environment Faculty Fostering Funding Gene Delivery Genes Genetic Genomics Goals Grant HIV-1 Histology Hospitals Imaging Techniques Immunologics Immunology In Vitro Infection Institution Institutional Review Boards Interdisciplinary Study Knockout Mice Laboratories Leadership Maintenance Mammalian Cell Marketing Medical Medicine Mentors Mentorship Methodology Methods Microelectrodes Microglia Microscopy Molecular Molecular Profiling Molecular and Cellular Biology Monitor Neurologic Neurons Neuropsychology Neurosciences Oligodendroglia Patients Peripheral Philadelphia Physicians Preparation Proteomics Protocols documentation Reagent Recruitment Activity Reporting Research Research Infrastructure Research Personnel Research Support Sampling Scientist Seeds Services Source Specialist Specimen Strategic Planning Subfamily lentivirinae System T-Lymphocyte Techniques Technology Testing Tissues Training Training Programs Transgenic Animals Translational Research Universities University Hospitals Viral Virus Virus Diseases antiretroviral therapy base behavior test behavioral study biomarker discovery brain endothelial cell career cognitive performance cohort college design differential expression genome analysis genome editing humanized mouse in vitro Model in vivo innovation interdisciplinary collaboration macrophage medical schools meetings metabolomics nerve stem cell neuroAIDS neurobehavior neurocognitive disorder neurotropic virus novel novel strategies operation phosphoproteomics programs public health relevance recombinant viral vector response therapeutic gene training opportunity viral DNA virology virus genetics

项目摘要

DESCRIPTION (provided by applicant): Neurologic complications and related diseases, including HIV-1 associated neurocognitive disorders or HAND, remain one of the most devastating clinical manifestations of HIV-1 infection even after treatment with antiretroviral therapy. Accordingly, it is now well recognized that a novel strategy is needed to eradicate AIDS and its associated co-morbidities as current antiretroviral therapies have failed to eliminate HIV-1 from the infected host. Here, we seek support to continue operation of a highly needed Comprehensive NeuroAIDS Core Center (CNAC) that was established in 2011 at the Temple University School of Medicine in partnership with Drexel University College of Medicine in Philadelphia to provide infrastructure for performing innovative research in the neuroscience of HIV-1/AIDS. The central theme of CNAC, in the second round of funding, will be to foster an environment for performing translational research in neuroAIDS by offering expertise and service in molecular and cellular biology as well as the genetics and virology of HIV-1 infection of the CNS using in vitro, in vivo, and ex vivo systems provided by the various cores. It is our goal that CNAC promotes research toward understanding mechanisms responsible for HAND and provide new paths for the eradication of infected cells and viruses and the treatment of AIDS/CNS disease. Accordingly, more emphasis will be on cell- and viral-based methodologies and the assessment of the impact of HIV-1 on cell function pertaining to CNS diseases. To accomplish our goal, the CNAC will provide expertise in neural cell cultures, viral reagents, microelectrode array, gene editing strategies, and high end proteomics/metabolomics through its Basic Science Core I (BSC I). Basic Science Core II (BSC II) will provide expertise and facilities in the employment of animal models for studying HIV-1 brain interaction and behavioral testing/training and histological training and related services. The Clinical and Translational Research Support Core (CTRSC) of the CNAC, with greater than 2,000 patients participating in two well-characterized/controlled cohorts at Temple University Hospital (North Philadelphia) and Drexel's Hahnemann Hospital (Center City Philadelphia) will support development and maintenance of cohesive IRB protocols, offer expertise in cognitive performance and neuropsychology, virology/immunology and immunoactivation, and viral genetics/genomics and bioinformatics. Through the Developmental Core, the CNAC will offer a unique opportunity for training and mentoring of junior investigators and clinical investigators, and attract and develop physicians/scientists in the field of neuroAIDS. The CNAC will provide infrastructure by i) providing start-up funds through its Developmental Core, ii) offering intellectual support and technical services through its state-of-the-art basic science cores and iii) access to an expansive well-operating clinical core, for conducting innovative research toward the development of intelligent, effective, and safe genetic and cellular/immunologic strategies toward a cure of AIDS. Further, the funding through CNAC will create an environment for the development of collaborative research in neuroAIDS at Temple, Drexel and other medical institutions in the greater Philadelphia area, and extend its collaboration with other AIDS research centers in Philadelphia and the nation.

描述（由申请人提供）：神经并发症和相关疾病，包括 HIV-1 相关的神经认知障碍或 HAND，即使在抗逆转录病毒治疗后，仍然是 HIV-1 感染最具破坏性的临床表现之一。因此，现在已充分认识到这一点。由于目前的抗逆转录病毒疗法未能消除感染宿主体内的 HIV-1，因此需要一种新的策略来根除艾滋病及其相关的高度并发症。在此，我们寻求支持以继续开展所需的工作。综合神经艾滋病核心中心 (CNAC) 于 2011 年与费城德雷克塞尔大学医学院合作在天普大学医学院成立，旨在为 HIV-1/艾滋病神经科学的创新研究提供基础设施。 CNAC 在第二轮融资中，将通过提供分子和细胞生物学以及中枢神经系统 HIV-1 感染的遗传学和病毒学方面的专业知识和服务，营造一个进行神经艾滋病转化研究的环境。 CNAC 的目标是促进对 HAND 机制的研究，并为根除受感染的细胞和病毒以及治疗 AIDS/CNS 疾病提供新途径。因此，我们将更加重视基于细胞和病毒的方法以及 HIV-1 对中枢神经系统疾病相关细胞功能影响的评估。为了实现我们的目标，CNAC 将提供神经细胞培养、病毒试剂、基础科学核心 I (BSC I) 的微电极阵列、基因编辑策略和高端蛋白质组学/代谢组学将提供使用动物模型研究 HIV-1 大脑相互作用的专业知识和设施。 CNAC 的临床和转化研究支持核心 (CTRSC) 包括行为测试/培训和组织学培训及相关服务，超过 2,000 名患者参与了两个特征明确/受控的队列。天普大学医院（费城北部）和德雷克塞尔哈内曼医院（费城中心城市）将支持IRB协议的开发和维护，提供认知表现和神经心理学、病毒学/免疫学和免疫激活以及病毒遗传学/基因组学和生物信息学方面的专业知识。作为发展核心，CNAC 将为初级研究人员和临床研究人员提供培训和指导的独特机会，并吸引和培养神经艾滋病领域的医生/科学家。 CNAC 将通过以下方式提供基础设施：i) 通过其发展核心提供启动资金，ii) 通过其最先进的基础科学核心提供智力支持和技术服务，以及 iii) 获得广泛运行良好的临床核心，开展创新研究，开发智能、有效、安全的遗传和细胞/免疫策略，以治愈艾滋病。此外，CNAC 提供的资金将为坦普尔、德雷克塞尔和其他机构的神经艾滋病合作研究的发展创造环境。医疗机构在大费城地区，并扩大与费城和全国其他艾滋病研究中心的合作。