Immunopathology Core

免疫病理学核心

• 批准号: 8868128
• 负责人: Jerry Ritchey
• 金额: $ 13.89万
• 依托单位: OKLAHOMA STATE UNIVERSITY STILLWATER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8868128
• 关键词: Animal Model; Animals; Autopsy; Bacteriology; Basic Science; Biological Models; Cells; Cellular Immunity; Centers of Research Excellence; Clinical Pathology; Clinical Sciences; Color; Communicable Diseases; Confocal Microscopy; Cytokine Gene; Development; Diagnostic; Disease; Electron Microscopy; Elements; Ensure; Enzyme-Linked Immunosorbent Assay; Evaluation; Flow Cytometry; Foundations; Freezing; Funding; Gene Expression; Humoral Immunities; Image; Immune response; Immunofluorescence Immunologic; Immunohistochemistry; Immunology; Infectious Diseases Research; Inflammatory; Interdisciplinary Study; Lesion; Lung diseases; Medicine; Molecular Biology; Nature; Oklahoma; Paraffin Embedding; Pathologist; Pathology; Proteins; Recruitment Activity; Research; Research Design; Research Infrastructure; Research Personnel; Resources; Sampling; Serologic tests; Services; Staining method; Stains; Testing; Time; Tissue Banks; Tissue Stains; Tissues; Toxicology; Training; base; comparative; cytokine; enzyme linked immunospot assay; experience; immune function; immunopathology; in vitro Assay; programs; respiratory; success; tissue processing; virology

The specific aim of the Immunopathology Core Program (IPC) is to provide pathology and immunology support to COBRE and other Oklahoma Centerfor Respiratory and Infectious Diseases (OCRID) investigators. Services provided by the IPC include a broad range of pathology support including gross necropsy and tissue collection, tissue processing, sectioning of either frozen or paraffin-embedded tissues, routine and special stains, immunohistochemistry (IHC), immunofluorescence (IFC), and coordination of ancillary services (clinical pathology, bacteriology, virology, toxicology). Immune responses important in the development of disease are characterized by evaluation of cell-mediated immunity (ELISPOT, flow cytometry for intracellular IFNy) and phenotypic identification of inflammatory cells within lesions (IFC, IHC, flow cytometry). The IPC will also coordinate with the Animal Models Core (AMC) and Molecular Biology Core (MBC) to assist investigators with humoral immunity (serology, ELISA) and quantitation of cytokine gene expression (real-time PCR) respectively. To achieve this aim, the IPC is staffed by three board-certified pathologists with experience in animal models and respiratory/infectious disease research and will recruit an experienced, extensively cross-trained technician. The IPC has the resources in place to serve as the foundation and a strong institutional commitment to ensure development of the IPC into a self-sustaining facility integral to the research infrastructure of OCRID.

免疫病理学核心计划（IPC）的具体目的是为柯布雷和其他俄克拉荷马州的呼吸道和传染病（OCRID）研究人员提供病理和免疫学支持。 IPC提供的服务包括广泛的病理支持，包括大颗粒状和组织收集，组织加工，冷冻或石蜡包含的组织的切片，常规和特殊污渍，免疫组织化学（IHC），免疫荧光（IHC），IFC（IFC）以及协调 辅助服务（临床病理学，细菌学，病毒学，毒理学）。对疾病发展重要的免疫反应的特征是评估细胞介导的免疫（ELISPOT，用于细胞内IFNY的流式细胞仪）和病变（IFC，IHC，流式细胞仪）内炎症细胞的表型鉴定。 IPC还将与动物模型核心（AMC）和分子生物学核心（MBC）协调，以帮助研究人员进行体液免疫（血清学，ELISA）和细胞因子基因表达（实时PCR）的定量。为了实现这一目标，IPC由三位经过董事会认证的病理学家组成，具有动物模型和呼吸/传染病研究经验，并将招募一位经验丰富的，广泛的交叉训练的技术人员。 IPC拥有作为基础和强大的机构承诺的资源，以确保IPC发展为Ocrid研究基础设施不可或缺的自我维持设施。