Islet Procurement and Analysis Core

胰岛获取和分析核心

• 批准号: 8876651
• 负责人: Marcela Brissova
• 金额: $ 10.26万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8876651
• 关键词: Area; Biological Assay; Biology; Development; Diabetes Mellitus; Equipment; Hormones; Human; Image Analysis; Immunology; Individual; Insulin-Dependent Diabetes Mellitus; Islets of Langerhans; Islets of Langerhans Transplantation; Knowledge; Measurement; Morphology; Mus; Postdoctoral Fellow; Price; Procedures; Process; Quality Control; RNA; Reporting; Research; Research Personnel; Scanning; Services; Signal Transduction; Slide; System; Techniques; Technology; Training; base; experience; graduate student; islet; morphometry; pancreatic islet function; tool; transcription factor

The Islet Procurement and Analysis Core (IPA Core) was expanded and became a new core of the Vanderbilt DRTC in June 2007. Soon after that the IPA Core was integrated into the Vanderbilt Core Ordering & Reporting Enterprise System. Islet biology, development, and function are major research areas of the Vanderbilt DRTC with investigators studying a variety of islet-related processes ranging from intracellular signaling, the immunology of type 1 diabetes, islet-enriched transcription factors, to islet transplantation. Necessary components of many of their experimental paradigms are high quality, well-characterized pancreatic islets, and reliable assays of islet function and mass. The main objective of the IPA Core is to provide DRTC-affiliated investigators with high quality mouse pancreatic islets. In addition, the core assists investigators in studies of islet function that involve services such as islet culture, islet perifusion, static islet incubation and RNA isolation. An important component of the IPA Core services is training. The IPA Core staff will continue to provide training to graduate students and postdoctoral fellows thus allowing them to gain knowledge and understanding of procedures used in their research. Due to high demand among DRTC-affiliated investigators, the IPA Core added to its equipment Aperio whole slide scanning system and will expand its service portfolio for the assessment of islet mass/morphology in this competitive renewal cycle. This will not only broaden the current DRTC user base, but will also allow us to reach out to non-VUMC users and offer slide scanning and image analysis services. Utilizing Aperio whole slide scanning technology and image analysis tools, the IPA Core will develop standards for pancreatic islet morphometry and measurement of islet mass. By having a centralized facility, the IPA Core provides services to a larger base of investigators at a lower price than would otherwise be possible. Other advantages are quality control, the ability to develop new techniques and applications, the increased potential for collaborative interactions, and immediate access to individuals with a broad experience and knowledge in handling isolated islets.

胰岛采购和分析核心（IPA Core）被扩展，并于2007年6月成为Vanderbilt DRTC的新核心。不久之后，IPA Core被整合到Vanderbilt Core Ordering＆Reporting Enterprise Enterprise System中。胰岛生物学，开发和功能是范德比尔特DRTC的主要研究领域，研究人员研究了各种与胰岛相关的过程，从细胞内信号传导，1型糖尿病的免疫学，胰岛增强的转录因子的免疫学到胰岛移植。它们许多实验范式的必要组成部分是高质量，良好的胰岛和胰岛功能和质量的可靠测定法。 IPA核心的主要目的是为DRTC相关研究者提供高质量的小鼠胰岛。此外，核心协助研究者研究涉及胰岛培养，胰岛渗透，静态胰岛孵育和RNA分离等服务的胰岛功能的研究。 IPA核心服务的重要组成部分是培训。 IPA核心人员将继续为研究生和博士后研究员提供培训，从而使他们能够获得对研究中使用的程序的了解和理解。由于DRTC附属研究人员的需求量很高，IPA核心增加了其设备Aperio全幻灯片扫描系统，并将在此竞争性更新周期中扩大其服务组合以评估胰岛质量/形态。这不仅将扩大当前的DRTC用户群，而且还可以使我们与非VUMC用户联系，并提供幻灯片扫描和图像分析服务。 IPA Core利用Aperio全幻灯片扫描技术和图像分析工具，将制定胰岛形态计量学和胰岛质量测量的标准。通过拥有集中式设施，IPA Core以比以前可能更低的价格为更大的调查人员提供服务。其他优势是质量控制，开发新技术和应用的能力，增加协作互动的潜力以及与具有广泛经验和知识的个人的立即访问，以处理孤立的胰岛。