Biochemistry Core

生物化学核心

基本信息

批准号：
10259820
负责人：
Peter Vitiello
金额：
$ 32.45万
依托单位：
SANFORD RESEARCH/USD
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-01 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10259820
关键词：
Affinity Biochemical Process Biochemistry Biological Biotin Biotinylation Cells Centers of Research Excellence Chimeric Proteins Complement Complex Consult Consultations Custom Data Analyses Detection Disease Equipment Experimental Designs Faculty Fatty Acids Fees Funding Goals Health Human Human Resources Institutes Institution Label Ligase Lipids Maintenance Manuscripts Mass Fragmentography Mass Spectrum Analysis Methods Mission Mutation Nature Pediatric Research Phase Plasmids Preparation Protein Biochemistry Proteins Proteomics Protocols documentation Recording of previous events Research Research Personnel Research Project Grants Sampling Scientist Services Sterols Technology Time Training Validation Work base comparative design experience experimental study innovative technologies member novel protein protein interaction repository screening stem stem cells

项目摘要

PROJECT SUMMARY The Biochemistry Core supports projects associated with the Center for Pediatric Research by providing consultation and technical support for protein-protein interaction screening, multi-analyte detection, and lipid profiling. The Core will continue to perform BioID as a cutting-edge approach to screen for protein-protein interactions. Furthermore, the Core will expand its technical abilities and provide services for multi-analyte detection and quantification of proteins as well as gas chromatography-mass spectrometry (GC/MS) for detection and quantification of fatty acids and sterols. The Core will also provide assistance in the preparation of manuscripts and funding applications as they relate to the services provided. By providing these services in- house, the core will directly interface with the investigators during experimental design, protocol establishment, troubleshooting and execution as well as organize any further analysis outside of the core. The purpose of the Biochemistry Core is to support the goals of its users, namely independent scientists studying how biochemical processes on stem/progenitor cell plasticity influence the basis of human health and disease.

项目概要生物化学核心支持与儿科研究中心相关的项目，提供蛋白质-蛋白质相互作用筛选、多分析物检测和脂质的咨询和技术支持分析。 Core 将继续将 BioID 作为筛选蛋白质-蛋白质的尖端方法互动。此外，核心还将扩展其技术能力并为多分析物提供服务蛋白质的检测和定量以及气相色谱-质谱 (GC/MS) 检测以及脂肪酸和甾醇的定量。核心还将协助准备与所提供的服务相关的手稿和资助申请。通过提供这些服务- house，核心将在实验设计、方案建立、故障排除和执行以及组织核心之外的任何进一步分析。目的生物化学核心是支持其用户的目标，即研究生物化学如何进行的独立科学家干/祖细胞可塑性的过程影响人类健康和疾病的基础。