Touchscreen-compatible, real-time electrochemical sensing of SARS-CoV-2

触摸屏兼容的 SARS-CoV-2 实时电化学传感

• 批准号: 10260946
• 负责人: Suzie H. Pun
• 金额: $ 46.65万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-12-21 至 2022-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10260946
• 关键词: 2019-nCoV; Affinity; Air; Antibodies; Binding; Binding Proteins; Biosensing Techniques; Biosensor; COVID-19; COVID-19 detection; COVID-19 pandemic; COVID-19 patient; Car Phone; Cellular Phone; Cessation of life; Chemistry; Clinical; Contact Tracing; Coronavirus; Detection; Development; Devices; Disease Outbreaks; Early Diagnosis; Electric Capacitance; Electrodes; Electronics; Engineering; Evaluation; Exhibits; Face; Frequencies; Future; Glass; Goals; Hospitals; Immobilization; Individual; Infection; Label; Libraries; Measures; Middle East Respiratory Syndrome; Molecular Conformation; Natural regeneration; Oxidation-Reduction; Pathogen detection; Pattern; Performance; Preparation; Process; Reporting; Restaurants; SARS coronavirus; SARS-CoV-2 spike protein; SARS-CoV-2 transmission; Sampling; Sensitivity and Specificity; Signal Transduction; Specificity; Surface; System; Technology; Testing; Time; Touch sensation; Vaccines; Validation; Virion; Work; aptamer; base; cost; density; design; infection rate; instrumentation; nanofabrication; new technology; novel; prevent; secondary infection; sensor; touchscreen; transmission process; viral detection; viral transmission

PROJECT SUMMARY The SARS-CoV-2 coronavirus, the cause of the COVID-19 global pandemic, is efficiently spread and has reached over 27 million confirmed cases as of September 8, 2020. There is therefore an urgent need for new technologies that can provide early detection of virus, reducing the transmission and infection rate. The goal of this proposal is to develop an integrated biosensor- touchscreen that sensitivity reports surface contact with SARS-CoV-2. In our preliminary work, we have identified several aptamers that bind specifically to the envelope-anchored trimeric spike (S) protein of SARS-CoV-2, but not of SARS-CoV or MERS. In comparison to antibodies, aptamers are synthetic molecules that more thermally stable and lower cost while providing similar specificity and affinity of target binding. In this application, we propose to integrate aptamer-based biosensing of SARS-CoV-2 into a touchscreen device. Our main objectives are to 1) engineer conformation switching aptamers for electrochemical sensing of SARS-CoV-2 binding, 2) develop nanogap capacitive sensors as a uniquely complementary approach to capacitive touchscreen technology and 3) build and test an integrated biosensor and touchscreen array that can detect SARS-CoV-2 from patient samples. Successful completion of these aims will result in a novel automatic sensing platform for SARS-CoV-2. This technology could transform personal device touchscreens as well as to multi-user touchscreen devices in hospitals, airports, libraries, restaurants, for early detection, curbing transmission rates from secondary exposure. Importantly, the developed technology could be adapted for other electronic sensing platforms, and easily applied for future pathogen detection.

项目概要 导致 COVID-19 全球大流行的 SARS-CoV-2 冠状病毒正在有效传播 截至 2020 年 9 月 8 日，确诊病例已超过 2700 万例。 迫切需要能够及早发现病毒、减少感染的新技术 传播和感染率。该提案的目标是开发一种集成生物传感器 触摸屏的灵敏度报告与 SARS-CoV-2 的表面接触。在我们的前期工作中， 我们已经确定了几种与包膜锚定的三聚体刺突特异性结合的适体 (S) SARS-CoV-2 的蛋白质，但不是 SARS-CoV 或 MERS 的蛋白质。与抗体相比， 适体是合成分子，具有更高的热稳定性和更低的成本，同时提供 靶标结合的相似特异性和亲和力。在此应用中，我们建议集成 基于适配体的 SARS-CoV-2 生物传感进入触摸屏设备。我们的主要目标是 1) 设计用于 SARS-CoV-2 电化学传感的构象转换适体 结合，2）开发纳米间隙电容传感器作为独特的补充方法 电容式触摸屏技术以及 3) 构建和测试集成生物传感器和触摸屏 可以从患者样本中检测 SARS-CoV-2 的阵列。顺利完成这些目标 将产生一种新型的 SARS-CoV-2 自动传感平台。这项技术可以改变 个人设备触摸屏以及医院、机场、 图书馆、餐馆，以便及早发现，遏制二次接触造成的传播率。 重要的是，所开发的技术可以适用于其他电子传感平台， 并易于应用于未来的病原体检测。