Compact, Integrated EPR Spectrometer for Dynamic Nuclear Polarization

用于动态核极化的紧凑型集成 EPR 光谱仪

基本信息

批准号：
8781173
负责人：
Thorsten Maly
金额：
$ 18.56万
依托单位：
BRIDGE 12 TECHNOLOGIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-30 至 2016-07-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8781173
关键词：
Area Cell Nucleus Characteristics Communities Complex Crystallography Data Data Quality Development Devices Discipline Drug Industry Electron Spin Resonance Spectroscopy Electrons Ensure Frequencies Funding Home environment Hour Investments Label Laboratories Lead Magic Magnetism Mechanics Methods NMR Spectroscopy Nuclear Phase Physiologic pulse Price Process Proliferating Relaxation Research Research Activity Research Personnel Research Project Grants Resolution Sampling Scientist Signal Transduction Small Business Innovation Research Grant Source Spectrum Analysis Structure System Time United States National Institutes of Health Work base cost effective design high sensitivity probe improved instrument instrumentation interest irradiation macromolecule magnetic field programs prototype public health relevance research study small molecule solid state solid state nuclear magnetic resonance structural biology technology development tool two-dimensional usability

项目摘要

DESCRIPTION (provided by applicant): The proposed research focuses on the development of a compact, integrated high-field EPR spectrometer for DNP-enhanced solid-state, magic-angle-spinning (MAS) NMR spectroscopy. The spectrometer will allow researchers to record the EPR spectra and obtain valuable relaxation parameters of the DNP sample under the same conditions under which the DNP experiment is performed. In recent years, DNP has proven to be a robust method to increase signal intensities in NMR experiments in laboratories around the world and substantial progress has been made in adapting DNP for solid-state NMR spectroscopy. However, to understand and optimize the DNP process it is crucial to fully understand the EPR spectrum and relaxation parameters of the polarizing agent. Only a few home-built high-field spectrometers are available around the world. Therefore researchers often have to speculate about the EPR characteristics of their DNP sample. We propose to develop a cost-effective EPR spectrometer that can easily be adapted to cover the complete frequency range that is currently used in DNP spectroscopy. The design will be compact and turn-key so that the device can also be used by non experts. During Phase I of this project we will perform the complete mechanical and electrical design and fabricate a prototype EPR spectrometer operating at 263 GHz (400 MHz 1H). The successful development of this technology will provide researchers access to currently inaccessible EPR characteristics and will proliferate the development of new, optimized polarizing agents for DNP-NMR spectroscopy. This will be of large interest to many projects funded by the U.S. National Institutes of Health.

描述（由申请人提供）：拟议的研究重点是开发一种紧凑、集成的高场 EPR 光谱仪，用于 DNP 增强固态魔角旋转 (MAS) NMR 光谱。该光谱仪将使研究人员能够在进行 DNP 实验的相同条件下记录 EPR 光谱并获得 DNP 样品的有价值的弛豫参数。近年来，DNP 已被证明是一种在世界各地实验室的 NMR 实验中增加信号强度的可靠方法，并且在将 DNP 应用于固态 NMR 波谱方面已取得了实质性进展。然而，要了解和优化 DNP 工艺，充分了解偏振剂的 EPR 谱和弛豫参数至关重要。世界各地只有少数自制的高场光谱仪。因此，研究人员经常必须推测 DNP 样本的 EPR 特征。我们建议开发一种经济高效的 EPR 光谱仪，可以轻松适应覆盖目前 DNP 光谱中使用的完整频率范围。该设计将是紧凑且交钥匙的，因此非专家也可以使用该设备。在该项目的第一阶段，我们将执行完整的机械和电气设计，并制造工作频率为 263 GHz (400 MHz 1H) 的原型 EPR 光谱仪。该技术的成功开发将为研究人员提供目前无法获得的 EPR 特性，并将促进 DNP-NMR 光谱学新型、优化偏振剂的开发。这将引起美国国立卫生研究院资助的许多项目的极大兴趣。