Compact, Integrated EPR Spectrometer for Dynamic Nuclear Polarization

用于动态核极化的紧凑型集成 EPR 光谱仪

• 批准号: 8781173
• 负责人: Thorsten Maly
• 金额: $ 18.56万
• 依托单位: BRIDGE 12 TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-30 至 2016-07-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8781173
• 关键词: Area; Cell Nucleus; Characteristics; Communities; Complex; Crystallography; Data; Data Quality; Development; Devices; Discipline; Drug Industry; Electron Spin Resonance Spectroscopy; Electrons; Ensure; Frequencies; Funding; Home environment; Hour; Investments; Label; Laboratories; Lead; Magic; Magnetism; Mechanics; Methods; NMR Spectroscopy; Nuclear; Phase; Physiologic pulse; Price; Process; Proliferating; Relaxation; Research; Research Activity; Research Personnel; Research Project Grants; Resolution; Sampling; Scientist; Signal Transduction; Small Business Innovation Research Grant; Source; Spectrum Analysis; Structure; System; Time; United States National Institutes of Health; Work; base; cost effective; design; high sensitivity probe; improved; instrument; instrumentation; interest; irradiation; macromolecule; magnetic field; programs; prototype; public health relevance; research study; small molecule; solid state; solid state nuclear magnetic resonance; structural biology; technology development; tool; two-dimensional; usability; Area; Cell Nucleus; Characteristics; Communities; Complex; Crystallography; Data; Data Quality; Development; Devices; Discipline; Drug Industry; Electron Spin Resonance Spectroscopy; Electrons; Ensure; Frequencies; Funding; Home environment; Hour; Investments; Label; Laboratories; Lead; Magic; Magnetism; Mechanics; Methods; NMR Spectroscopy; Nuclear; Phase; Physiologic pulse; Price; Process; Proliferating; Relaxation; Research; Research Activity; Research Personnel; Research Project Grants; Resolution; Sampling; Scientist; Signal Transduction; Small Business Innovation Research Grant; Source; Spectrum Analysis; Structure; System; Time; United States National Institutes of Health; Work; base; cost effective; design; high sensitivity probe; improved; instrument; instrumentation; interest; irradiation; macromolecule; magnetic field; programs; prototype; public health relevance; research study; small molecule; solid state; solid state nuclear magnetic resonance; structural biology; technology development; tool; two-dimensional; usability

DESCRIPTION (provided by applicant): The proposed research focuses on the development of a compact, integrated high-field EPR spectrometer for DNP-enhanced solid-state, magic-angle-spinning (MAS) NMR spectroscopy. The spectrometer will allow researchers to record the EPR spectra and obtain valuable relaxation parameters of the DNP sample under the same conditions under which the DNP experiment is performed. In recent years, DNP has proven to be a robust method to increase signal intensities in NMR experiments in laboratories around the world and substantial progress has been made in adapting DNP for solid-state NMR spectroscopy. However, to understand and optimize the DNP process it is crucial to fully understand the EPR spectrum and relaxation parameters of the polarizing agent. Only a few home-built high-field spectrometers are available around the world. Therefore researchers often have to speculate about the EPR characteristics of their DNP sample. We propose to develop a cost-effective EPR spectrometer that can easily be adapted to cover the complete frequency range that is currently used in DNP spectroscopy. The design will be compact and turn-key so that the device can also be used by non experts. During Phase I of this project we will perform the complete mechanical and electrical design and fabricate a prototype EPR spectrometer operating at 263 GHz (400 MHz 1H). The successful development of this technology will provide researchers access to currently inaccessible EPR characteristics and will proliferate the development of new, optimized polarizing agents for DNP-NMR spectroscopy. This will be of large interest to many projects funded by the U.S. National Institutes of Health.

描述（由申请人提供）：拟议的研究重点是开发用于DNP增强固态，魔法旋转（MAS）NMR光谱的紧凑，高场EPR光谱仪。光谱仪将使研究人员能够记录EPR光谱，并在执行DNP实验的相同条件下获得DNP样品的有价值的弛豫参数。近年来，DNP已被证明是增加世界各地实验室NMR实验中信号强度的一种强大方法，并且在适应DNP以改编DNP来改编DNP来改编固态NMR光谱。但是，要了解和优化DNP过程，至关重要的是完全了解偏振剂的EPR光谱和松弛参数。世界各地只有少数几个家庭建造的高场光谱仪。因此，研究人员通常必须推测其DNP样本的EPR特征。我们建议开发一种具有成本效益的EPR光谱仪，该光谱仪可以很容易地适应以覆盖当前在DNP光谱法中使用的完整频率范围。该设计将是紧凑和交钥匙的，因此该设备也可以由非专家使用。在该项目的第一阶段，我们将执行完整的机械和电气设计，并在263 GHz（400 MHz 1H）下制造原型EPR光谱仪。该技术的成功开发将为研究人员提供当前无法接近的EPR特征，并将扩大新的，优化的极化剂为DNP-NMR光谱的开发。这对于由美国国立卫生研究院资助的许多项目引起了人们的关注。