A Resonator for Pulsed ODNP Spectroscopy to Study Surface Hydration Dynamics

用于研究表面水合动力学的脉冲 ODNP 光谱谐振器

• 批准号: 10325293
• 负责人: Thorsten Maly
• 金额: $ 49.18万
• 依托单位: BRIDGE 12 TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-12-28 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10325293
• 关键词: Alzheimer' s Disease; Area; Cell Nucleus; Cell physiology; Collaborations; Communities; Computer software; Data; Defect; Detection; Development; Dimensions; Disease; Electromagnetics; Electron Spin Resonance Spectroscopy; Electrons; Equipment; Evaluation; Fruit; Funding; Health; Heart; Heating; Home; Hydration status; Journals; Laboratories; Liquid substance; Maps; Mechanics; Membrane; Membrane Lipids; Membrane Proteins; Methods; NMR Spectroscopy; Non-Insulin-Dependent Diabetes Mellitus; Nuclear; Parkinson Disease; Performance; Phase; Physiologic pulse; Physiological Processes; Play; Process; Proliferating; Protein Dynamics; Proteins; Pythons; Research; Research Activity; Research Personnel; Resolution; Resources; Role; Sampling; Signal Transduction; Site; Small Business Innovation Research Grant; Solid; Spectrum Analysis; Spin Labels; Structure; Surface; Techniques; Technology; Time; United States National Institutes of Health; Universities; Water; X-Ray Crystallography; biophysical techniques; cost effective; design; electron diffraction; experimental study; improved; instrument; instrumentation; interest; irradiation; macromolecule; microwave electromagnetic radiation; novel; open source; protein structure; prototype; simulation; solid state nuclear magnetic resonance; structural biology; success; technology development; three dimensional structure

Project Summary / Abstract The proposed research focuses on the development of a turn-key resonator for liquid-state Overhauser Dynamic Nuclear Polarization (ODNP) spectroscopy to study the site-specific translational dynamics of water molecules located at the interface of bio-macromolecules such as membrane proteins. It will allow researchers to readily perform ODNP experiments in either a state-of-the-art commercially available X-band cw/pulsed electron paramagnetic resonance (EPR) spectrometer or using a benchtop ODNP spectrometer. In recent years, DNP has proven to be a robust method to increase signal intensities in NMR experiments in laboratories around the world and substantial progress has been made in adapting DNP for solid- and solution- state NMR spectroscopy. This progress has sparked a new interest in ODNP spectroscopy. Although the method is known since the 1960s it has just recently been applied successfully to study the site-specific translational dynamics of water located at the interface of large bio-macromolecules such as membrane proteins. ODNP can map out the local and site-specific hydration dynamics landscape of membrane proteins and lipid membranes and can provide critical information about the protein structure and dynamics. One of the major challenges in ODNP spectroscopy is microwave induced sample heating. During Phase I of this project, we successfully designed, fabricated and characterized a novel prototype ODNP resonator. This SBIR Phase II application will assure that the prototype can be turned into a customer product. As demonstrated by the prototype, the resonator will have a much higher microwave conversion factor compared to conventional rectangular or circular EPR cavities. In addition, the low Q resonance structure will allow pulsed ODNP experiments to further minimize microwave induced heating by reducing the average power required to saturate the EPR transitions. The successful development of this technology will provide researchers access to instrumentation allowing them to incorporate ODNP spectroscopy in their research routine without the hassle of troubleshooting home-built equipment. This will greatly proliferate the method and is of large interest to many projects funded by the U.S. National Institutes of Health.

项目摘要 /摘要 拟议的研究重点是开发用于液态过度大冲突器的旋转器 动态核极化（ODNP）光谱学研究了水的特定位置翻译动力学 位于生物大分子（例如膜蛋白）界面上的分子。它将允许研究人员 很容易在商业上最先进的X波段CW/脉冲的最先进的ODNP实验 电子顺磁共振（EPR）光谱仪或使用台式ODNP光谱仪。 近年来，DNP已被证明是增加NMR实验信号强度的强大方法 在全球实验室中 状态NMR光谱。这一进展激发了ODNP光谱的新兴趣。虽然方法 自1960年代以来就知道它最近已成功地用于研究特定地点的翻译 位于大生物大分子（例如膜蛋白）界面的水的动力学。 ODNP可以 绘制膜蛋白和脂质膜的局部和特定位置水合动力学景观 并可以提供有关蛋白质结构和动力学的关键信息。 ODNP光谱法的主要挑战之一是微波引起的样品加热。在阶段 在这个项目中，我们成功设计，制造和表征了一种新型的ODNP谐振器。这 SBIR II期应用程序将确保原型可以变成客户产品。如证明 通过原型，与传统 矩形或圆形EPR腔。此外，低Q共振结构将允许脉冲ODNP 实验通过减少饱和所需的平均功率来进一步最小化微波引起的加热 EPR过渡。 该技术的成功开发将为研究人员提供仪器的访问 允许他们将ODNP光谱纳入其研究常规，而无需进行故障排除 家居设备。这将极大地扩散该方法，并引起许多资助的许多项目的兴趣 美国国立卫生研究院。