Overhauser Enhanced Magnetic Resonance Imaging (OMRI)

奥豪瑟增强磁共振成像 (OMRI)

• 批准号: 8175327
• 负责人: murali cherukuri
• 金额: $ 42.12万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8175327
• 关键词: Anatomy; Biochemical; Blood Vessels; Cell Nucleus; Chemicals; Contrast Media; Goals; Hypoxia; Image; Label; Magnetic Resonance Imaging; Magnetism; Metabolic; Monitor; Mus; Nature; Oxygen; Pattern; Perfusion; Pericytes; Protons; Pyruvate; Pyruvates; Pyruvic Acid; Resolution; Signal Transduction; Sirolimus; Tissues; Vascular Permeabilities; Water; imaging modality; in vivo; irradiation; molecular imaging; ratiometric; research study; response; treatment response; tumor

a) Profile tumors in terms of tumor vascular permeability and oxygenation using in vivo hyperpolarization of water at low fields. Hyperpolarization is usually carried out with the paramagnetic molecule TAM. From the image intensity profiles we obtained tumor oxygen and tumor vascular permeability simultaneously and correlated with immunohistochemical characterization of tumors. We found that tumor hypoxia was associated with increased tumor vascular permeability which in turn was negatively correlated with vascular pericyte coverage. b) Monitor the in vivo breakdown patterns of hyperpolarized (ex vivo) 13C-labeled pyruvic acid in tumors as indicators of treatment response: In this study, we injected 13C labeled pyruvate and monitored the conversion to lactate. The ratiometric images lactate/pyruvate can provide information on response to treatment if the ratios decrease. We used this imaging modality to evaluate treatment response in SCC VII tumors in mice treated with rapamycin and sunitinib. Preliminary results show that 13C imaging monitoring the breakdown of lactate/pyruvate was predictive in treatment response.

a）在肿瘤血管通透性和使用体内高极化的水平高极化方面，谱图肿瘤。 超极化通常用顺磁分子TAM进行。从图像强度曲线中，我们同时获得了肿瘤氧和肿瘤血管通透性，并与肿瘤的免疫组织化学表征相关。我们发现肿瘤缺氧与肿瘤血管通透性增加有关，这反过来又与血管周细胞覆盖率负相关。 b）监测肿瘤中超极化（体内）13C标记的丙酮酸的体内分解模式，作为治疗反应的指标：在这项研究中，我们注射了13C标记的丙酮酸标记并监测向乳酸的转化。如果比率降低，乳酸/丙酮酸的比率图像可以提供有关治疗反应的信息。我们使用这种成像方式来评估用雷帕霉素和舒尼替尼治疗的小鼠SCC VII肿瘤中的治疗反应。初步结果表明，监测乳酸/丙酮酸分解的13C成像在治疗反应中是可预测的。