Project 1: Microbiology of ME/CFS

项目1：ME/CFS微生物学

• 批准号: 10246406
• 负责人: W. Ian Lipkin
• 金额: $ 75.65万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-22 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10246406
• 关键词: Address; Agonist; Antibiotics; Antibodies; Antiviral Agents; Autoantibodies; B-Lymphocytes; Bacteria; Base Sequence; Biological Assay; Biological Markers; Blood; Chronic Fatigue Syndrome; Communities; Data; Diagnosis; Disease; Double-Stranded RNA; Failure; Herpesviridae; High-Throughput Nucleotide Sequencing; Immune Plasma; Immune response; Immunity; Infection; Infectious Agent; Inflammation; Intervention; Intravenous infusion procedures; Laboratories; Lead; Methods; Microbe; Microbiology; Monoclonal Antibodies; Natural Immunity; Nucleic Acids; Oral; Pathogenesis; Patient observation; Patients; Peptides; Peripheral Blood Mononuclear Cell; Plasma; Polymerase Chain Reaction; Prevalence; Probiotics; Reporting; Rest; Risk Factors; Role; Running; Sampling; Serology; Shotguns; Surveys; TLR3 gene; Vertebrate Viruses; Virus; Virus Replication; Work; analog; animal model development; bacterial community; bacteriome; base; case control; clinical subtypes; cytokine; density; detection method; dysbiosis; fecal transplantation; fungus; immune function; insight; metabolomics; metagenomic sequencing; microbial; mycobiome; novel; pathogen; response; transcriptome sequencing; virome

Project 1 Microbiology of ME/CFS Abstract Many patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) report a prodrome consistent with infection and/or inflammation. Up to 40% of patients are reported to respond to intravenous infusions of the Toll-like receptor 3 agonist, Ampligen, a double-stranded RNA analogue proposed to inhibit viral replication. Others report responses to antiviral drugs specific for herpesviruses, pre- and probiotics or fecal transplantation, or monoclonal antibodies that deplete B cells. We hypothesize that at least some ME/CFS patients have an infectious trigger for their disease, and that failures to implicate infectious agents reflect inadequate sampling and/or inappropriate assays. To explore the role of infection and immunity in ME/CFS, we will exploit sensitive sequence-based methods for detection and characterization of bacteria, viruses, and fungi, using blood, oral, and fecal samples from well characterized ME/CFS cases and controls. This project has the potential to lead to the development of animal models based on dysbiosis as well as the identification of patients with ME/CFS who may benefit from antiviral, antibiotic, or probiotic interventions.

项目1 ME/CFS 微生物学 抽象的 许多肌痛性脑脊髓炎/慢性疲劳综合征 (ME/CFS) 患者报告有前驱症状 与感染和/或炎症一致。据报道，高达 40% 的患者对静脉注射有反应 Toll 样受体 3 激动剂 Ampligen 的输注，Ampligen 是一种双链 RNA 类似物，旨在抑制 病毒复制。其他人报告了对疱疹病毒特异性抗病毒药物、益生菌和益生菌或 粪便移植或消耗 B 细胞的单克隆抗体。我们假设至少有一些 ME/CFS 患者的疾病有传染性触发因素，但未能发现传染源 反映采样不足和/或分析不适当。探讨感染和免疫在疾病中的作用 ME/CFS，我们将利用敏感的基于序列的方法来检测和表征细菌， 病毒和真菌，使用来自明确特征的 ME/CFS 病例和对照的血液、口腔和粪便样本。 该项目有可能导致基于生态失调的动物模型的开发以及 识别可能受益于抗病毒、抗生素或益生菌干预措施的 ME/CFS 患者。