Acquisition of a Pulse Electron Paramagnetic Resonance Spectrometer

脉冲电子顺磁共振波谱仪的采集

• 批准号: 7840304
• 负责人: Peter Z Qin
• 金额: $ 103.99万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-07 至 2011-12-06
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7840304
• 关键词: Alzheimer' s Disease; American; Area; Arts; Award; Cell Nucleus; Chromosomal translocation; Communities; Complex; DNA-Directed DNA Polymerase; Electron Nuclear Double Resonance; Electron Spin Resonance Spectroscopy; Electrons; Funding; Grant; Human; Infection; Institution; Ions; Ligands; Los Angeles; Maps; Measurement; Measures; Metals; Minority; Mission; Molecular; Molecular Conformation; Monitor; Nitrogenase; Occupations; Physiologic pulse; Public Health; Research; Spectrum Analysis; Structure; System; Training; Transcriptional Regulation; United States National Institutes of Health; Viral; biological systems; cofactor; instrument; protein transport; tool

DESCRIPTION (provided by applicant): The bio-molecular research community in the Los Angeles area is requesting NIH funding to purchase a state-of-art pulse electron paramagnetic resonance (EPR) spectrometer, the EleXsys FT-EPR system from Bruker Corporation. Pulse EPR spectroscopy is a powerful tool for analyzing conformations and metal ion cofactors of biological systems. The proposed pulse EPR spectrometer simultaneously will provide two capabilities that are central to the participating users. One is Double Electron Electron Resonance (DEER) spectroscopy, which selectively measures electron spin dipolar interactions to yield inter-spin distances up to 80 ¿ in bio-molecules. The measured distances provide direct structural constraints for mapping global structures and monitoring conformational changes of complex bio-molecular systems. User projects that require DEER span many important public health related areas, including viral replication and infection (X. Chen, Goodman, Qin), protein trafficking (Shan), transcription regulation (L. Chen), and human chromosomal translocation (Lieber). The second type of pulse EPR measurement is Electron Nuclear Double Resonance (ENDOR) and Electron Spin Echo Envelope Modulation (ESEEM), which measure interactions between the electron spin and the surrounding nuclei. This will be used to study ligand identity and coordination geometry of metal co-factors in nitrogenase (Rees, McKenna), DNA polymerase (McKenna, Crans, Goodman), and amoyloid formation in Alzheimer's disease (Zhou). Pulse EPR capabilities are grossly inadequate at the participating institutions. Funding of the proposed instrument will significantly benefit on-going research in the group of users, which are currently supported by 23 NIH grants. It will also support the broader regional research community, training of minorities, and educational missions. The award will immediately impact approximately 500 jobs in American, thus stimulating the economy.

描述（由申请人提供）：洛杉矶地区的生物分子研究界正在请求 NIH 资助购买最先进的脉冲电子顺磁共振 (EPR) 光谱仪，即布鲁克公司的 EleXsys FT-EPR 系统。 EPR 光谱是分析生物系统构象和金属离子辅助因子的强大工具，所提出的脉冲 EPR 光谱仪将同时提供对参与项目至关重要的两种功能。一种是双电子电子共振 (DEER) 光谱，它有选择地测量电子自旋偶极相互作用，以产生高达 80 ¿测量的距离为绘制全局结构和监测复杂生物分子系统的构象变化提供了直接的结构约束，这些项目需要 DEER 跨越许多重要的公共卫生相关领域，包括病毒复制和感染（X. Chen， Goodman，Qin）、蛋白质运输（Shan）、转录调控（L. Chen）和人类染色体易位（Lieber） 脉冲 EPR 测量的第二种类型是电子核双共振（ENDOR）和电子。自旋回波包络调制 (ESEEM)，测量电子自旋与周围原子核之间的相互作用，这将用于研究固氮酶（Rees、McKenna）、DNA 聚合酶（McKenna、Crans）中金属辅因子的配体身份和配位几何结构。 ），古德曼），以及阿尔茨海默病中的淀粉样蛋白形成（周），参与机构的 Pulse EPR 能力严重不足，这将大大有利于用户组正在进行的研究。该奖项目前由 23 项 NIH 拨款支持，还将支持更广泛的地区研究界、少数族裔培训和教育使命，该奖项将立即影响美国约 500 个就业岗位，从而刺激经济。

项目成果

Phosphorothioate-Based Site-Specific Labeling of Large RNAs for Structural and Dynamic Studies.

• DOI: 10.1021/acschembio.2c00199
• 发表时间: 2022-09-07
• 期刊: ACS chemical biology
• 影响因子: 4
• 作者: Yanping Hu;Yan Wang;Jaideep Singh;Ruirui Sun;Lilei Xu;Xiaolin Niu;K. Huang;Guangcan Bai
• 通讯作者: Guangcan Bai

Low temperature synthesis and characterization of lanthanide-doped BaTiO3 nanocrystals.

稀土掺杂 BaTiO3 纳米晶的低温合成及表征。

• DOI: 10.1039/c3cc49575b
• 发表时间: 2014-03-04
• 期刊: Chemical communications
• 影响因子: 4.9
• 作者: Sean P. Culver;V. Stepanov;Matthew Mecklenburg;Susumu Takahashi;R. Brutchey
• 通讯作者: R. Brutchey

Investigating Functional DNA Grafted on Nanodiamond Surface Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance Spectroscopy.

使用定点自旋标记和电子顺磁共振波谱研究接枝到纳米金刚石表面的功能性 DNA。

• DOI: 10.1021/acs.jpcb.6b00790
• 发表时间: 2016-04-20
• 期刊: The journal of physical chemistry. B
• 作者: Rana Akiel;Xiaojun Zhang;C. Abeywardana;V. Stepanov;P. Qin;Susumu Takahashi
• 通讯作者: Susumu Takahashi

CRISPR-Cas9 Mediated DNA Unwinding Detected Using Site-Directed Spin Labeling.

使用定点自旋标记检测 CRISPR-Cas9 介导的 DNA 解旋。

• 发表时间: 2017-06-16
• 影响因子: 4
• 作者: Tangprasertchai, Narin S;Di Felice, Rosa;Zhang, Xiaojun;Slaymaker, Ian M;Vazquez Reyes, Carolina;Jiang, Wei;Rohs, Remo;Qin, Peter Z
• 通讯作者: Qin, Peter Z

Posttranscriptional site-directed spin labeling of large RNAs with an unnatural base pair system under non-denaturing conditions.

在非变性条件下使用非天然碱基对系统对大 RNA 进行转录后定点自旋标记。

• 发表时间: 2020-09-21
• 影响因子: 8.4
• 作者: Wang, Yan;Kathiresan, Venkatesan;Chen, Yaoyi;Hu, Yanping;Jiang, Wei;Bai, Guangcan;Liu, Guoquan;Qin, Peter Z;Fang, Xianyang
• 通讯作者: Fang, Xianyang