UCLA IDDRC: Structural and Functional Visualization Core

加州大学洛杉矶分校 IDDRC：结构和功能可视化核心

基本信息

批准号：
10085985
负责人：
SAMANTHA J BUTLER
金额：
$ 18.45万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10085985
关键词：
3-Dimensional Affect Animal Model Animals Axon Biological Biological Models Biological Process Brain Brain region Cell Culture Techniques Cell physiology Cells Complex Coupled Data Dendrites Developmental Disabilities Disease Fluorescence Microscopy Gene Expression Profiling Generations Genetic Human Image In Vitro Intellectual and Developmental Disabilities Research Centers Laboratories Learning Light Light Microscope Magnetic Resonance Imaging Memory Methodology Methods Microscope Microscopy Modality Modeling Molecular Probes Mus Nervous System Physiology Neurons Optics Organ Organoids Patients Pattern Photons Population Protocols documentation Recording of previous events Research Research Personnel Research Support Resolution Resources Rodent Rodent Model Role Sampling Scientist Services Sleep disturbances Spectrum Analysis Stem Cell Research Structure Techniques Technology Therapeutic Intervention Tissues Translating Visual Acuity Visualization Work animal imaging brain circuitry cognitive neuroscience community center developmental disease fluorescence microscope holistic approach improved in vivo imaging light microscopy member miniaturize nervous system development neural circuit neural implant new technology next generation novel novel imaging technology open source optical imaging optogenetics relating to nervous system stem cells submicron two-photon voucher

项目摘要

CORE E: STRUCTURAL AND FUNCTIONAL VISUALIZATION Samantha Butler, Core Director; Peyman Golshani, Core Co-Director; Neil Harris and Susan Bookheimer; MRI sub-core directors Abstract The Structural and Functional Visualization Core provides comprehensive imaging services to the members of UCLA Intellectual & Developmental Disabilities Research Center (IDDRC), working on any aspect of the genetic and environmentally-induced developmental diseases affecting nervous system development and function. The research ongoing in the UCLA IDDRC spans basic scientists using reductionist approaches to elucidate the mechanisms specific to intellectual developmental disabilities (IDDs) to clinicians assessing therapeutic interventions for patients. To accommodate all of their imaging requirements, we provide access to three light microscopy cores, including a microscopy suite dedicated to IDDRC researchers, human and animal MRI facilities and the technical support needed to initiate and complete any imaging analysis. The Structural and Functional Visualization Core also works to develop new technologies for visualizing biological samples and in turn provide them to IDDRC researchers. In this proposal, we are focused on [1] developing smaller lighter one-photon miniaturized fluorescent microscopes for live imaging neural activity in freely moving animals and [2] refining the methods for CLARITY and iDISCO, protocols that render tissue transparent thereby permitting unparalleled visual acuity into the complex circuitry of the brain. These techniques offer the promise of a holistic approach to imaging, permitting IDDRC researchers to translate mechanism into therapy. For example, researchers investigating a specific intellectual disorder will be able perform MRI on patients to identify the affected region of the brain, implant miniaturized microscopes in rodent models to perform Ca2+ imaging in vivo to examine how the firing patterns of specific populations of neuron are mechanistically altered by the disease, while concomitantly examining putative aberrant circuit formation using light microscopy coupled with CLARITY. Finally, this core also supports the efforts of all the other cores, offering IDDRC researchers the ability to both probe molecular and cellular function at any level from the sub- cellular to living animals and determine the consequence of therapeutic interventions.

核心 E：结构和功能可视化萨曼莎·巴特勒，核心总监； Peyman Golshani，核心联合总监；尼尔·哈里斯和苏珊·布克海默； MRI 子核心主任抽象的结构和功能可视化核心为加州大学洛杉矶分校智力与发育障碍研究中心 (IDDRC) 成员，正在工作影响遗传和环境诱发的发育疾病的任何方面神经系统的发育和功能。加州大学洛杉矶分校 IDDRC 正在进行的研究涵盖基础科学家使用还原论方法来阐明智力的特定机制发育障碍 (IDD) 供临床医生评估患者的治疗干预措施。到满足他们所有的成像要求，我们提供三光学显微镜核心，包括专用于 IDDRC 研究人员、人类和动物 MRI 的显微镜套件启动和完成任何成像分析所需的设施和技术支持。结构和功能可视化核心还致力于开发新技术用于可视化生物样本并将其提供给 IDDRC 研究人员。在这个提案中，我们专注于 [1] 开发更小、更轻的单光子微型荧光显微镜用于自由活动动物的实时成像神经活动并 [2] 改进 CLARITY 方法和 iDISCO，使组织透明的协议，从而实现无与伦比的视敏度进入大脑的复杂电路。这些技术提供了整体方法的希望成像，使 IDDRC 研究人员能够将机制转化为治疗。例如，调查特定智力障碍的研究人员将能够对患者进行核磁共振检查识别受影响的大脑区域，在啮齿动物模型中植入微型显微镜进行体内 Ca2+ 成像，以检查特定神经元群体的放电模式被疾病机械地改变，同时检查假定的异常电路使用光学显微镜结合 CLARITY 形成。最后，该核心还支持所有其他核心的工作，提供 IDDRC 研究人员能够在任何水平上探测分子和细胞功能细胞到活体动物并确定治疗干预的结果。