The Gastrointestinal Experimental Model Systems (GEMS) Core

胃肠实验模型系统 (GEMS) 核心

• 批准号: 10117232
• 负责人: NOAH Freeman SHROYER
• 金额: $ 17.6万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-01 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10117232
• 关键词: 3-Dimensional; Advisory Committees; Animals; Barrett Esophagus; Biological Models; Biology; Cancer Patient; Cell Culture Techniques; Cells; Clinical; Collection; Colonic Adenoma; Colorectal Cancer; Consultations; Culture Media; Derivation procedure; Development; Differentiation and Growth; Digestive System Disorders; Disease; Education and Outreach; Experimental Models; Generations; Germ-Free; Gnotobiotic; Goals; Health; Hospitals; Human; Immigrant; Immunodeficient Mouse; Inflammatory Bowel Diseases; Institutional Review Boards; Instruction; Insurance; Intestines; Maintenance; Medical center; Medicine; Modeling; Mus; Organoids; Patients; Pediatrics; Physiology; Privatization; Production; Protocols documentation; Published Comment; Reagent; Recommendation; Research Personnel; Rodent; Sampling; Services; Specific qualifier value; Specimen; Surveys; Technology; Texas; Tissue Sample; Training; Uninsured; Veterans; Xenograft procedure; animal facility; animal tissue; base; biobank; college; cost; embryonic stem cell; gastrointestinal; human pluripotent stem cell; human tissue; improved; induced pluripotent stem cell; matrigel; member; monolayer; neoplastic; patient population; pre-clinical; success; two-dimensional

Project Summary The Gastrointestinal Experimental Model Systems (GEMS) core is a newly reorganized of the Texas Medical Center-Digestive & Disease Center (TMC-DDC) in 2015. Based on examination of prior usage, reviewer comments from the previous competitive renewal, and the recommendation of the external advisory committee, the GEMS core is organized to encompass 1) an enteroid/organoid subcore and 2) a gnotobiotic subcore. The DDC successfully obtained Baylor College of Medicine (BCM) support to establish a gnotobiotic animal facility during the previous Project Period. This reorganization was based on the exciting scientific developments in the field, our unique expertise, increased DDC investigator need and use of enteroids and gnotobiotic animals in conjunction with decreased use of the physiology services offered by the previous Integrative Biology Core. To accomplish these aims, we will provide services, including the following: • Human enteroids from the TMC-DDC biobank: as 3-dimensional enteroids, monolayers or transwells • Murine enteroids from the TMC-DDC biobank • Complete growth media or components for growth and differentiation of human and animal enteroids • Derivation of new primary enteroids from animals or human tissue specimens • Derivation of stably transduced enteroid lines • Human pluripotent stem cell-derived intestinal organoids, made from embryonic stem cells or iPS cells • Xenograft generation of enteroids/organoids into immunodeficient mice • Production and maintenance of germ-free rodents • Technical services for studies involving gnotobiotic rodents • Creation of germ-free mice de novo • Training, outreach, and consultative services Importantly, there is no other similar facility in the TMC or nearby in the region. This Core is highly synergetic with the other scientific cores of the TMC-DDC. For example, extensive cooperation with Core E (Clinical Core) facilitates the development and approval of new IRB protocols and collection/tracking of tissue samples required to establish new human enteroid lines. Evidence of the success of this interaction is apparent in the >120 enteroids lines that are already established and the continued accrual of new samples targeting specific patient populations.

项目概要 胃肠实验模型系统 (GEMS) 核心是德克萨斯州新重组的 医疗中心-消化和疾病中心 (TMC-DDC)，2015 年。根据之前使用情况的检查， 之前竞争性续展的审稿人意见以及外部的推荐 咨询委员会中，GEMS 核心的组织包括 1) 肠类/类器官子核心和 2) DDC 成功获得贝勒医学院 (BCM) 支持建立。 上一个项目期间的一个限生动物设施这次重组是基于 该领域令人兴奋的科学发展、我们独特的专业知识、增加的 DDC 研究者需求和使用 肠类动物和知生动物的减少以及所提供的生理学服务的使用减少 为了实现这些目标，我们将提供服务，包括 下列： • 来自 TMC-DDC 生物库的人肠类：3 维类肠、单层或跨孔 • 来自 TMC-DDC 生物库的鼠类肠蛋白 • 用于人类和动物肠类生长和分化的完整生长培养基或成分 • 从动物或人体组织标本中衍生出新的原代肠类 • 稳定转导的肠样线的衍生 • 人类多能干细胞衍生的肠道类器官，由胚胎干细胞或 iPS 制成 细胞 • 将肠类/类器官异种移植到免疫缺陷小鼠中 • 无菌啮齿动物的生产和维护 • 涉及限生啮齿动物研究的技术服务 • 从头创造无菌小鼠 • 培训、外展和咨询服务 重要的是，TMC 或该地区附近没有其他类似的设施。 与 TMC-DDC 的其他科学核心协同作用 例如，与核心的广泛合作。 E（临床核心）促进新 IRB 协议的开发和批准以及收集/跟踪 建立新的人类肠类细胞系所需的组织样本。这种相互作用成功的证据。 显而易见的是已经建立了超过 120 个肠类产品线，并且不断增加新产品 针对特定患者群体的样本。