Protein Interaction Core

蛋白质相互作用核心

• 批准号: 8337371
• 负责人: DAVID G. MYSZKA
• 金额: $ 5.94万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8337371
• 关键词: Area; Binding; Biological; Biosensor; Computer software; Core Facility; Data Analyses; Development; Drops; Electronic Mail; Ensure; Equipment; Funding; Grant; HIV; Health Sciences; Human Resources; Image; Label; Laboratories; Lipids; Measures; Oligonucleotides; Optics; Process; Proteins; Proteome; Research Personnel; Research Project Grants; Resolution; Sampling; Schedule; Surface; Surface Plasmon Resonance; System; Technology; Time; United States National Institutes of Health; Universities; Utah; Work; base; design; instrument; medical schools; meetings; protein protein interaction; research study; sensor; small molecule; square foot; structural biology; trafficking

Overview Our Protein Interaction Core will tap into an existing Core facility that is part of the University of Utah's Health Sciences Core facilities. The Protein Interaction Core facility was established in 1996 to provide internal and external academic users access to state-of-the-art, label-free, real-time interaction technologies. Over the past 10 years, this facility has contributed to more than 100 research projects that encompass a wide array of biological areas. The Protein Interaction Core is centrally located within the School of Medicine (Rm 4A416) on the Health Sciences campus. The laboratory's 1600 square feet of space was entirely renovated in 1997 and was specifically designed to accommodate the Core facility's equipment and personnel. Currently, the facility maintains 7 surface plasmon resonance-based optical biosensors: 3 Biacore 2000s, 1 Biacore 3000, 1 Biacore S51, 1 Biacore Flexchip, and 1 Lumera Proteome Processor. The Biacore 2000/3000 platforms are ideal for measuring protein-protein interactions, while the Biacore S51 is designed for small-molecule interaction analysis. The Flexchip and Proteome Processor instruments are array-based imaging systems that allow one to study up to 1000 binding partners immobilized onto the sensor surface at one time. The Core has worked closely with both Biacore and Lumera on the development of this new hardware, as well as data analysis software. In early 2007, we plan to add a new biosensor (the ProteOn XPR36 from BioRad Laboratories) to our Core, funded by ab NIH Shared Instrument Grant. Together, these state-of-the-art instruments allow us to measure the interactions of biomolecules such as proteins, oligonucleotides, and lipids in high-resolution, as well as high-throughput, formats. Experiments are scheduled through email contacts with the Core's manager. Typically, the investigator and their staff then meet with the manager to plan the best course of action for the proposed study. Samples are then dropped off at the Core and experiments are initiated the same day. This ensures that labile samples are able to be processed in a timely manner.

概述 我们的蛋白质相互作用核心将利用现有的核心设施，该设施是犹他大学的一部分 健康科学核心设施。蛋白质相互作用核心设施成立于 1996 年，旨在提供 内部和外部学术用户可以访问最先进的、无标签的实时交互 技术。过去 10 年来，该设施为 100 多个研究项目做出了贡献 涵盖广泛的生物领域。 蛋白质相互作用核心位于医学院（Rm 4A416）的中心位置 健康科学校区。实验室1600平方英尺的空间于1997年进行了全面翻修 专为容纳核心设施的设备和人员而设计。现在， 该设施拥有 7 个基于表面等离子共振的光学生物传感器：3 个 Biacore 2000s、1 个 Biacore 3000、1 个 Biacore S51、1 个 Biacore Flexchip 和 1 个 Lumera 蛋白质组处理器。 Biacore 2000/3000 平台非常适合测量蛋白质-蛋白质相互作用，而 Biacore S51 则专为测量蛋白质相互作用而设计。 小分子相互作用分析。 Flexchip 和蛋白质组处理器仪器基于阵列 成像系统允许研究多达 1000 个固定在传感器上的结合伙伴 一次浮出水面。 The Core 与 Biacore 和 Lumera 密切合作开发 这个新的硬件，以及数据分析软件。 2007年初，我们计划增加一个新的生物传感器 （来自 BioRad 实验室的 ProteOn XPR36）到我们的核心，由 ab NIH 共享仪器资助 授予。这些最先进的仪器共同使我们能够测量生物分子的相互作用 例如高分辨率和高通量格式的蛋白质、寡核苷酸和脂质。 实验是通过与核心经理的电子邮件联系来安排的。通常情况下，调查员 然后，他们的员工会与经理会面，为拟议的研究规划最佳行动方案。 然后样本被送到核心并在同一天开始实验。这确保了 能够及时处理不稳定样品。