MULTI-PHOTON BIOSENSOR DEVELOPMENT CORE

多光子生物传感器开发核心

基本信息

批准号：
8726960
负责人：
RICHARD N DAY
金额：
$ 15.78万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8726960
关键词：
Address Adenovirus Vector Apoptosis Attention Behavior Biomedical Research Biosensor Calcium Cell Culture Techniques Cell physiology Cells Cellular biology Characteristics Cloning Collaborations Cyclic AMP Development Engineering Event Florida Fluorescence Resonance Energy Transfer Functional disorder Future Genetic Engineering Goals Image Indiana Kidney Label Life Mammalian Cell Medicine Methods Microscopic Microscopy Mission Modeling Modification Optics Organism Penetration Photobleaching Photons Physiology Plasmid Cloning Vector Plasmids Play Property Proteins Protocols documentation Renal function Reporter Reporting Research Personnel Resolution Role Signal Transduction Testing Tissues Universities Variant Vertebral column base cellular imaging comparative design fluorophore imaging probe in vivo in vivo imaging insight intravital microscopy multi-photon novel ratiometric rho GTP-Binding Proteins theories tool two-photon vector

项目摘要

The cloning and engineering of genetically encoded fluorescent proteins has allowed the development of many unique tools for studies in cell biology, medicine, and physiology. When combined with intravital microscopy, these probes have the potential to report events from inside living cells in the intact organism. However, the utility of the fluorescent protein biosensors for intravital microscopy has been limited by the poorly characterized behaviors of fluorescent proteins under two-photon excitation. The goal of the Multiphoton Biosensor Development core is to develop and rigorously validate a set of biosensor probes optimized for intravital multiphoton microscopy studies of renal function. Modular probes developed by the core will be incorporated into a toolkit of probes and protocols that will be provided to renal investigators. These probes will provide renal researchers with powerful new tools that will enable them to conduct novel intravital microscopy studies addressing fundamental issues of renal physiology, cell biology and pathophysiology.

遗传编码的荧光蛋白的克隆和工程允许开发许多用于细胞生物学，医学和生理学研究的独特工具。当与浸润显微镜结合使用时，这些探针可能会报告完整生物体中活细胞内部的事件。然而，在两光子激发下，荧光蛋白的荧光蛋白行为较差，荧光蛋白生物传感器对浸润液显微镜的实用性受到限制。多光子生物传感器发育核心的目的是开发和严格验证一组针对肾功能内内内多光子显微镜研究优化的生物传感器探针。核心开发的模块化探针将被纳入将提供给肾脏研究人员的探针和协议的工具包中。这些探针将为肾脏研究人员提供强大的新工具，使他们能够进行新的插入式显微镜研究，以解决肾脏生理，细胞生物学和病理生理学的基本问题。