SPECIFIC REPRESSION OF PROLACTIN GENE EXPRESSION

催乳素基因表达的特异性抑制

• 批准号: 2444055
• 负责人: RICHARD N DAY
• 金额: $ 13.23万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-11 至 1998-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2444055
• 关键词: DNA footprinting; RNA binding protein; RNA splicing; estradiol; estrogen receptors; gel mobility shift assay; genetic promoter element; genetic regulatory element; hormone regulation /control mechanism; immunoprecipitation; laboratory rat; neoplasm /cancer transplantation; northern blottings; nuclear runoff assay; prolactin; repression; site directed mutagenesis; solution hybridization; somatotropin; tissue /cell culture; transcription factor; transfection; western blottings

DESCRIPTION (adapted from the applicant's abstract): The objective of the proposed studies is to identify the critical molecular events that direct specific repression of PRL gene expression. Aim 1 is to establish the importance of the alternative splice form of Pit-1 in the repression of PRL and to determine the molecular events that contribute to the splice choice. Aim 2 is to determine if specific DNA elements in the PRL gene are the key determinants of why PRL gene expression is inhibited in GH3 cell-induced tumors, while GH gene expression is not. Aim 3 will be to examine the interaction of proteins with the PRL and GH promoters, and to characterize the proteins that are involved in the specific repression of the PRL gene.

描述（根据申请人的摘要改编）： 拟议的研究是确定关键分子事件 PRL基因表达的直接抑制。 目标1是 确定pit-1的替代剪接形式的重要性 抑制PRL并确定贡献的分子事件 剪接选择。 AIM 2是确定是否在 PRL基因是为什么PRL基因表达为何的关键决定因素 在GH3细胞诱导的肿瘤中抑制，而GH基因表达却没有。 目标3将是检查蛋白质与PRL的相互作用 GH启动子，并表征参与的蛋白质 PRL基因的具体抑制。