MicroRNA & Breast Cancer: Functional Characterization in a Population-Based Study
微小RNA
基本信息
- 批准号:8744266
- 负责人:
- 金额:$ 62.85万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2013
- 资助国家:美国
- 起止时间:2013-09-30 至 2018-08-31
- 项目状态:已结题
- 来源:
- 关键词:AccountingAnimal ModelApoptosisArchivesBiological AssayBiological MarkersBreastBreast Cancer CellCancer PrognosisCandidate Disease GeneCell DeathCell LineCell ProliferationCell physiologyCellsCharacteristicsChemopreventive AgentClassificationClinicalComplexCustomDNA MethylationDataDatabasesDevelopmentDietDiseaseEpidemiologic StudiesEpidemiologyEtiologyExogenous FactorsFunctional disorderGene Expression RegulationGene TargetingGenetic Predisposition to DiseaseGenomeGenomicsGuiltIn VitroInvestigationKnowledgeLaboratory FindingLeadLife StyleLinkLong Island Breast Cancer StudyMalignant NeoplasmsMammary NeoplasmsMessenger RNAMethodsMicroRNAsMolecularMolecular ProfilingNatureNeoplasm MetastasisOncogenicOutcomePathogenesisPatientsPhenotypePopulationPopulation StudyPost-Transcriptional RegulationProcessPrognostic MarkerPublic HealthPublicationsReproductive HistoryResearch DesignRoleSample SizeSamplingSourceStreamSystemTechnologyThe Cancer Genome AtlasTherapeuticTimeTumor Suppressor ProteinsTumor TissueValidationbasebreast tumorigenesiscancer cellcancer cell differentiationcancer diagnosiscancer genomecell growthdeep sequencingdesignfollow-upgene discoveryhistone modificationimprovedin vitro Assayinfancymalignant breast neoplasmmatrigelnovelpopulation basedpublic health relevancereconstructionstemstemnesstreatment strategytumortumor initiationtumor progression
项目摘要
DESCRIPTION (provided by applicant): Despite considerable advancement in our knowledge about the significant role of microRNA (miRNA) in fundamental cellular processes related to cancer, most data come from in vitro/animal models or population studies with limited scope. There is a critical need for systematic investigation on the role of miRNA in breast cancer in well-designed population studies that take into account the complexity of miRNA functions and multifactorial nature of breast cancer. Herein, we have designed a hypothesis-driven, technology-enabled translational project aimed at systematically elucidating the role of miRNA in breast cancer survival. Taking advantage of the latest results from the Cancer Genome Atlas (TCGA) and our improved deep sequencing method, we will validate/identify breast cancer dysregulated miRNAs in breast tumors of representative clinical subtypes. We will employ multiple in vitro assays to characterize these miRNAs for their potential in cell growth/proliferation, differentiation, and cancer cell stemness, as well as to identify their down-stream targets in breast cell lines. We will then independently validate the laboratory findings in
a population-based epidemiologic study, the Long Island Breast Cancer Study Project (LIBCSP). The unique strength of the proposed study is our ability to incorporate latest genomic technology (deep sequencing), functional molecular methods (in vitro assays), in which phenotypic relevance of candidate miRNAs can be characterized, with classic epidemiology (population-based study), in which complex exogenous factors and patient information can be taken into account. This multi-scale, multi-disciplinary, and multi-directional approach allows a better characterization of the causal relationship between miRNA and breast cancer development and progression. This project has the real potential to identify a miRNA signature that predicts breast cancer outcomes.
描述(由申请人提供):尽管我们了解microRNA(miRNA)在与癌症有关的基本细胞过程中的重要作用方面有了很大的进步,但大多数数据来自体外/动物模型或范围有限的人群研究。迫切需要系统地研究miRNA在乳腺癌中的作用在精心设计的人群研究中,这些研究考虑了miRNA功能的复杂性和乳腺癌的多因素性质。本文中,我们设计了一个由假设驱动的,以技术为基础的翻译项目,旨在系统地阐明miRNA在乳腺癌生存中的作用。利用癌症基因组图集(TCGA)的最新结果和我们改进的深度测序方法,我们将验证/鉴定乳腺癌失调的乳腺癌失调的miRNA在代表性临床亚型的乳腺肿瘤中。我们将使用多种体外测定法来表征这些miRNA,以表征它们在细胞生长/增殖,分化和癌细胞干的潜力,并确定其在乳腺细胞系中的下游靶标。然后,我们将独立验证实验室发现
一项基于人群的流行病学研究,长岛乳腺癌研究项目(LIBCSP)。拟议研究的独特强度是我们能够纳入最新的基因组技术(深度测序),功能性分子方法(体外测定),其中可以通过经典的流行病学(基于人群的研究)来表征候选miRNA的表型相关性,在哪种复杂的外源性因素和患者信息中可以考虑到其中。这种多尺度,多学科和多方向方法可以更好地表征miRNA与乳腺癌发展和进展之间的因果关系。该项目具有确定预测乳腺癌预后的miRNA特征的真正潜力。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Jia Chen其他文献
SIFT and Preserving Topology Structures of Local Neighborhood: Matching Feature Point in Deformation Measurement of Nonrigid Biological Tissues from Magnetic Resonance Images
SIFT 与保留局部邻域拓扑结构:磁共振图像非刚性生物组织变形测量中的特征点匹配
- DOI:
10.1166/jmihi.2015.1427 - 发表时间:
2015-06 - 期刊:
- 影响因子:0
- 作者:
Jia Chen;Xubing Zhang - 通讯作者:
Xubing Zhang
Jia Chen的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Jia Chen', 18)}}的其他基金
Characterizing the functional genomic atlas of human placenta and unveiling the prenatal programming of early-life development
表征人类胎盘的功能基因组图谱并揭示早期生命发育的产前编程
- 批准号:
10580294 - 财政年份:2023
- 资助金额:
$ 62.85万 - 项目类别:
MicroRNA & Breast Cancer: Functional Characterization in a Population-Based Study
微小RNA
- 批准号:
8912879 - 财政年份:2013
- 资助金额:
$ 62.85万 - 项目类别:
MicroRNA & Breast Cancer: Functional Characterization in a Population-Based Study
微小RNA
- 批准号:
9333257 - 财政年份:2013
- 资助金额:
$ 62.85万 - 项目类别:
MicroRNA & Breast Cancer: Functional Characterization in a Population-Based Study
微小RNA
- 批准号:
8630725 - 财政年份:2013
- 资助金额:
$ 62.85万 - 项目类别:
Breast Cancer Genomics in Windows of Susceptibility to Endocrine Disruptors
乳腺癌基因组学对内分泌干扰物的易感性窗口
- 批准号:
8665931 - 财政年份:2010
- 资助金额:
$ 62.85万 - 项目类别:
Breast Cancer Genomics in Windows of Susceptibility to Endocrine Disruptors
乳腺癌基因组学对内分泌干扰物的易感性窗口
- 批准号:
8461235 - 财政年份:2010
- 资助金额:
$ 62.85万 - 项目类别:
相似国自然基金
丁苯酞通过调节细胞异常自噬和凋亡来延缓脊髓性肌萎缩症动物模型脊髓运动神经元的丢失
- 批准号:82360332
- 批准年份:2023
- 资助金额:31.00 万元
- 项目类别:地区科学基金项目
利用可视可控hypocretin神经元凋亡的疾病模型进行发作性睡病发病机制研究
- 批准号:81901346
- 批准年份:2019
- 资助金额:20.5 万元
- 项目类别:青年科学基金项目
组织器官衰老致退行性演变多示踪剂全身动态PET显像研究
- 批准号:91949121
- 批准年份:2019
- 资助金额:68.0 万元
- 项目类别:重大研究计划
Fascin-2基因TALEN敲除小鼠渐进性听力减退的机制研究
- 批准号:81771020
- 批准年份:2017
- 资助金额:54.0 万元
- 项目类别:面上项目
Slug参与CP-31398介导的p53突变型子宫内膜癌细胞凋亡的机制研究
- 批准号:81702967
- 批准年份:2017
- 资助金额:19.0 万元
- 项目类别:青年科学基金项目
相似海外基金
Decoding the Paradox of DDX41-mutant MDS
解读 DDX41 突变型 MDS 的悖论
- 批准号:
10905168 - 财政年份:2023
- 资助金额:
$ 62.85万 - 项目类别:
Characterization of aneuploidy, cell fate and mosaicism in early development
早期发育中非整倍性、细胞命运和嵌合体的表征
- 批准号:
10877239 - 财政年份:2023
- 资助金额:
$ 62.85万 - 项目类别:
Novel Stellate Ganglia Chemo-ablation Approach to Treat Cardiac Arrhythmia and Cardiac Remodeling in Heart Failure
新型星状神经节化疗消融方法治疗心律失常和心力衰竭心脏重塑
- 批准号:
10727929 - 财政年份:2023
- 资助金额:
$ 62.85万 - 项目类别:
Resolvin D1 resolves inflammation in metabolic stress associated HFpEF
Resolvin D1 解决代谢应激相关 HFpEF 中的炎症
- 批准号:
10533087 - 财政年份:2022
- 资助金额:
$ 62.85万 - 项目类别: