Diagnostic Influenza RT-PCR Array

诊断流感 RT-PCR 芯片

• 批准号: 8094348
• 负责人: DARRELL P CHANDLER
• 金额: $ 98.56万
• 依托单位: AKONNI BIOSYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2012-12-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8094348
• 关键词: Area; Centers for Disease Control and Prevention (U.S.); Cessation of life; Clinical; Communicable Diseases; Computer software; Data Analyses; Data Reporting; Detection; Development; Diagnosis; Diagnostic; Disease; Disease Outbreaks; Elements; Far East; Goals; Health; Hospitalization; Hospitals; Hour; Image; Influenza; Influenza A Virus, H5N1 Subtype; Institution; Microarray Analysis; Performance; Peripheral; Pharmaceutical Preparations; Phase; Preparation; RNA Viruses; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Study Section; Swab; System; Technology; Testing; United States Food and Drug Administration; Universities; Viral; blind; cost; influenzavirus; pandemic disease; particle; pre-clinical; research study

DESCRIPTION (provided by applicant): Influenza viruses are highly contagious negative-sense RNA viruses cause approximately 114,000 hospitalizations and 20,000 deaths annually. Several drugs are approved by the US Food and Drug Administration (FDA) for treating influenza infections, but successful treatment depends on knowing the specific antigenic subtype and administering these drugs within the first 48 h of illness. Recent outbreaks of avian influenza H5N1 in East Asia have fueled concern over another pandemic and emphasize the need for comprehensive strain sub-typing in surveillance and diagnostic applications. The objective of this Phase 2 Project is therefore to develop and field test a sample-to-answer RT-PCR array kit for influenza detection and antigenic sub-typing in diagnostic and surveillance settings, with an emphasis on simple, low-cost microarray technology that can be deployed and used in peripheral hospital labs. The performance goal is a sample-to answer result in 2 hours or less at an input target concentration of 102 viral particles per mL nasopharyngeal swab. Early adopters at Wadsworth Center, Columbia University, Little Company of Mary Hospital and the CDC will assist in the pre-clinical verification of the technology on amended and blind clinical samples. PUBLIC HEALTH RELEVANCE: The influenza RT-PCR array will be the first-of-its-kind combination of PCR and microarray technology in a simple, inexpensive test kit for disease surveillance and diagnosis. The underlying platform developed herein will likewise find broad application in many areas of infectious disease diagnostics.

描述（由申请人提供）：流感病毒是高度传染性的阴性RNA病毒，每年可导致约114,000例住院和20,000人死亡。美国食品药品监督管理局（FDA）批准了几种药物来治疗流感感染，但成功的治疗取决于了解特定的抗原亚型，并在疾病的前48小时内施用这些药物。最近在东亚发生的禽流感H5N1爆发引起了人们对另一个大流行的关注，并强调了在监视和诊断应用中需要全面的应变亚型。因此，该第2阶段项目的目的是开发和现场测试样品到撤回的RT-PCR阵列试剂盒，用于在诊断和监视环境中用于流感检测和抗原亚型，重点是可以在外围医院实验室中部署和使用的简单，低成本的微阵列技术。性能目标是在2小时或更少的情况下以每ML鼻咽拭子102个病毒颗粒的输入目标浓度在2小时或更少的情况下进行样本答案。沃兹沃思中心，哥伦比亚大学，玛丽医院的小公司和CDC的早期采用者将在修订和盲目的临床样本上进行临床前验证该技术。公共卫生相关性：流感RT-PCR阵列将是PCR和微阵列技术的首先组合，以用于疾病监测和诊断的简单，廉价的测试套件。本文开发的基础平台同样将在许多传染病诊断领域中找到广泛的应用。

项目成果

Development and clinical testing of a simple, low-density gel element array for influenza identification, subtyping, and H275Y detection.

开发和临床测试一种简单的低密度凝胶元件阵列，用于流感鉴定、亚型分型和 H275Y 检测。

• DOI: 10.1016/j.jviromet.2014.07.019
• 发表时间: 2014
• 期刊: Journal of virological methods
• 影响因子: 3.1
• 作者: Chandler,DarrellP;Griesemer,SaraB;Knickerbocker,Christopher;Golova,JuliaB;Lambarqui,Amine;Perov,AlexanderN;Zimmerman,Cynthia;Wiles,Cory;Rudy,GeorgeB;StGeorge,Kirsten
• 通讯作者: StGeorge,Kirsten

Integrated Amplification Microarrays for Infectious Disease Diagnostics.

用于传染病诊断的集成扩增微阵列。

• DOI: 10.3390/microarrays1030107
• 发表时间: 2012
• 期刊: Microarrays (Basel, Switzerland)
• 作者: Chandler,DarrellP;Bryant,Lexi;Griesemer,SaraB;Gu,Rui;Knickerbocker,Christopher;Kukhtin,Alexander;Parker,Jennifer;Zimmerman,Cynthia;George,KirstenSt;Cooney,ChristopherG
• 通讯作者: Cooney,ChristopherG