THE PHOTOSYNTHETIC CHROMATOPHORE

光合色素细胞

• 批准号: 8363661
• 负责人: DARRELL P CHANDLER
• 金额: $ 4.97万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-09-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363661
• 关键词: Bioinformatics; Biophysics; Charge; Chemicals; Chromatophore; Complex; Diffuse; Funding; Grant; Harvest; Light; Membrane; Modeling; National Center for Research Resources; Organelles; Photons; Principal Investigator; Process; Production; Proteins; Proteobacteria; Protons; Quinones; Reaction; Research; Research Infrastructure; Resources; Rhodobacter sphaeroides; Shapes; Source; Tube; United States National Institutes of Health; Vesicle; absorption; cost; dimer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The chromatophore is a pseudo-organelle that is responsible for harvesting light and subsequent conversion of light energy to chemical energy in the form of ATP (http://www.ks.uiuc.edu/Research/psures). After initial excitation from photon absorption by the light harvesting proteins in the chromatophore, excitation energy is tranferred in a multi-step process to a reaction center (RC). At the RC the excitation energy is used to charge a quinone molecule, which then diffuses through the protein-rich chromatophore membrane to a bc$_1$. There the charged quinone is oxidized to produce a proton gradient across the membrane, which in turn drives ATP production by ATP synthase. In purple bacteria, such as Rhodobacter sphaeroides, the protein responsible for photon capture are known as light harvesting complex 1 (LH1) and light harvesting complex 2 (LH2). LH1 associates with two other proteins found in the chromatophore, RC and PufX, to form LH1- RC-PufX dimer complexes. LH2 and LH1-RC-PufX have a dual function; not only are they responsible for capturing light energy, they also induce membrane curvature to shape the chromatophore into, e.g., vesicles or tubes. Therefore, the systemis relevant to understand two fundamental questions in biophysics, namely membrane morphonegensis and cellular energy production.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 染色体是一种伪轨道，负责收获光和 随后以ATP形式将光能转化为化学能 （http://www.ks.uiuc.edu/research/psures）。从光子吸收初始激发后 染色体中的光收集蛋白，激发能在A中转换 多步过程到反应中心（RC）。在RC，激发能量用于 充电喹酮分子，然后通过富含蛋白质的染色体扩散 膜到BC $ _1 $。在那里，带电的喹酮被氧化以产生质子梯度 整个膜又通过ATP合酶驱动ATP生产。紫色 细菌，例如杜鹃花sphaeroides，负责光子捕获的蛋白质是 被称为光收集复合物1（LH1）和光收集复合物2（LH2）。 LH1 与在染色体RC和PUFX中发现的另外两种蛋白相关，形成LH1-- RC-PUFX二聚体复合物。 LH2和LH1-RC-PUFX具有双重函数；他们不仅是 负责捕获光能，它们还诱导膜曲率形成 染色体成囊泡或管。因此，系统与理解有关 生物物理学中的两个基本问题，即膜摩电话和细胞 能源生产。