Flow Cytometry Shared Resource

流式细胞术共享资源

基本信息

批准号：
10022769
负责人：
Remi J Creusot
金额：
$ 12.54万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-07-04 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10022769
关键词：
Cancer Biology Cancer Center Support Grant Cell Separation Cell surface Cells Color Complex Computer software Computers and Advanced Instrumentation Conceptions Consultations Data Data Analyses Development Doctor of Philosophy Equipment Event Flow Cytometry Flow Cytometry Shared Resource Funding Goals Hematopoietic Herbert Irving Comprehensive Cancer Center Hour Human Resources Immune Immunology Infrastructure Intracellular Signaling Proteins Journals Lasers Leadership Liquid substance Lymphoid Cell Maintenance Malignant Neoplasms Manuscripts Metabolic Marker Myeloid Cells Nature Peer Review Population Preparation Protocols documentation Publications Reproducibility Research Research Personnel Research Project Grants Research Support Sampling Scientist Secure Services Solid Sorting - Cell Movement Speed Stains Techniques Technology Time Training Training and Education Trees United States National Institutes of Health Walking anticancer research base cancer cell cancer immunotherapy cancer therapy complex data cost effective cost effectiveness data acquisition density design detector empowered experience high dimensionality high standard high throughput screening indexing insight instrument instrumentation leukemia/lymphoma member multidimensional data neoplastic cell new technology novel programs prospective service providers stem cells success technology development technology validation tool transcription factor tumor tumor immunology

项目摘要

FLOW CYTOMETRY SHARED RESOURCE: PROJECT SUMMARY The Flow Cytometry Shared Resource (FCSR) gives access to flow cytometry analysis and cell sorting instruments and services to Herbert Irving Comprehensive Cancer Center (HICCC) members. Under the leadership of Remi Creusot, PhD, the FCSR provides: (1) multi-color panel flow data acquisition and analysis using flow cytometry analyzers, including high throughput screening (HTS), (2) flow cytometry cell sorting (four- way or six-way or plate) using high speed cell sorters by dedicated operators of FCSR, including single cell index sorting, and (3) comprehensive consultation, panel design, hands-on training, troubleshooting, and data analysis services by PhD-level FCSR scientists. This broad and inclusive package of services enables HICCC investigators to analyze tumor cell populations and their microenvironment, including the tumor-infiltrating lymphoid and myeloid cells at the single cell level. During the current project period (2014-2019), the FCSR has been integrated with the flow cytometry core of the Columbia Center for Translational Immunology (CCTI). The merging of these facilities has secured access to a broader range of state-of-the-art instrumentation and brought advanced expertise in immunology to the research of HICCC flow cytometry users. HICCC members now have access to nine instruments, including five analyzers and four sorters. In addition, two of the existing instruments/analyzers have been upgraded to 18-20 detectors. Moreover, a new Influx sorter has been acquired to expand the capacity of sorting services. Upgrades in consultation services have further empowered the research of HICCC members who now benefit from face-to-face consultation on panel design, protocol optimization, and hands-on training on data acquisition, analysis, and experimental troubleshooting essential to maximize the impact of new advanced instruments for high dimensional multi-color panels. Based on its advanced instrumentation, highly qualified staff, broad portfolio of specialized services, easy accessibility, and cost-effective services, the FCSR is the flow cytometry service provider of choice for HICCC members. The FCSR will continue to incorporate the latest technologies and to provide high quality cell sorting, analytical and consultation services to HICCC members to empower their understanding of cancer biology and to enable new breakthroughs in cancer therapy. Over the current project period, the capabilities of the FCSR have been utilized by 81 HICCC members, provided key data and insights to support the success of more than 46 HICCC member peer-reviewed publications, including 23 contributions in journals with impact factor >10, of which 12 were in journals with an impact factor >20 (Nature, Cell Stem Cell, Cancer Cell, Cancer Discovery), and currently support research for 21 NIH-funded research grants (11 from NCI).

流式细胞术共享资源：项目摘要流式细胞术共享资源 (FCSR) 提供流式细胞术分析和细胞分选的访问权限为赫伯特·欧文综合癌症中心 (HICCC) 成员提供仪器和服务。下在 Remi Creusot 博士的领导下，FCSR 提供：(1) 多色面板流数据采集和分析使用流式细胞术分析仪，包括高通量筛选（HTS），（2）流式细胞术细胞分选（四路或六路或板）使用由 FCSR 专门操作员提供的高速细胞分选机，包括单细胞索引 (3)综合咨询、面板设计、实操培训、故障排除、数据分析由博士级 FCSR 科学家提供的服务。这种广泛而包容的服务包使 HICCCC 研究人员分析肿瘤细胞群及其微环境，包括肿瘤浸润单细胞水平上的淋巴和骨髓细胞。在当前项目期间（2014-2019），FCSR 已与哥伦比亚转化免疫学中心 (CCTI) 的流式细胞术核心集成。这这些设施的合并确保了获得更广泛的最先进仪器，并带来了为 HICCCC 流式细胞术用户的研究提供先进的免疫学专业知识。 HICCC 会员现在拥有使用九台仪器，包括五台分析仪和四台分选机。此外，现有的两个仪器/分析仪已升级至18-20个检测器。此外，还购买了一台新的 Influx 分拣机扩大分拣服务能力。咨询服务升级进一步赋能 HICCC 成员的研究，他们现在受益于面板设计、协议的面对面咨询数据采集、分析和实验故障排除方面的优化和实践培训对于最大限度地发挥新的先进仪器对高维多色面板的影响。基于其先进的仪器、高素质的员工、广泛的专业服务组合、便捷的服务以及性价比高的服务，FCSR是HICCC会员首选的流式细胞术服务提供商。这 FCSR将继续采用最新技术并提供高质量的细胞分选、分析和为 HICCC 成员提供咨询服务，以增强他们对癌症生物学的理解并启用新的癌症治疗的突破。在当前项目期间，FCSR 的功能已得到利用由 81 名 HICCCC 成员提供，提供了关键数据和见解，支持超过 46 名 HICCC 成员取得成功同行评审出版物，包括 23 篇影响因子 >10 的期刊论文，其中 12 篇发表在影响因子>20的期刊（Nature、Cell Stem Cell、Cancer Cell、Cancer Discovery），目前支持获得 21 项 NIH 资助的研究资助（其中 11 项来自 NCI）。