Single Cell Spatial Analysis in Tissue

组织中的单细胞空间分析

• 批准号: 10701699
• 负责人: Jared Kyle Burks
• 金额: $ 25.84万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-09 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10701699
• 关键词: Biological Assay; Biological Sciences; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cell Communication; Cell Separation; Cells; Collaborations; Complex; Comprehensive Cancer Center; Consultations; Core Facility; Cytometry; Education; Ensure; Equipment; Experimental Designs; Faculty; Flow Cytometry; Growth; Hour; Image; Immune; Industry; Institution; Methodology; Microscope; Modeling; Process; Proteomics; Publications; Reporting; Research; Research Personnel; Services; Source; Standardization; Suspensions; Technical Expertise; Technology; Testing; Tissues; Training; University of Texas M D Anderson Cancer Center; anticancer research; cancer genomics; cellular imaging; flexibility; imaging facilities; imaging system; instrumentation; liquid crystal polymer; member; neoplastic cell; new technology; novel; programs; single cell technology; spectrograph; stem; success; tool

Project Summary/Abstract Flow Cytometry, Mass Cytometry, Cell Sorting, and Imaging are essential tools for a wide range of methodologies aimed at understanding complex single cell proteomic and genomic cancer research. The instrumentation involved is large, complex, and can be difficult to maintain and operate. As such, a targeted core facility is critical for a Comprehensive Cancer Center such as MD Anderson. The FCCIF, est. 1982, provides these services. Since the last CCSG renewal (2012) 404 cancer center members from all 16 cancer center programs have used the facility and produced 604 publications. Many of the hot topics in cancer biology currently stem from immune cell interaction with the tumor cell; immunephenotyping is an in demand field of research that requiring a unique high plex assay. This requires a specialized imaging or cytometry approach and the FCCIF has specifically acquired two different platforms because of their diversity and flexibility. These platforms are the Vectra Multispectral imaging system and the CyTOF (suspension and imaging) Mass Cytometers. The FCCIF provides a common source of state-of-the-art expertise and technical skills to support the varied research efforts of MD Anderson investigators. The FCCIF provides a comprehensive service to all of the institutional investigators regardless of prior expertise. Dr. Jared K. Burks (author of this R50, co-Director of the FCCIF-NC) provides critical services including: 1) education, 2) consultation on needed technological approach, 3) training on equipment use, analytics, and proper experimental design. I identify and acquire novel technologies for use by MDACC investigators and I provide support in a manner to ensure success and mitigate pitfalls. I am expected to develop new strategies and technologies to meet the research needs of our faculty. A few examples of what I have currently been working on is the Vectra Polaris Multispectral Microscope, Helios and Hyperion Suspension and Imaging CyTOFs. The Polaris has operated on average 13.5 hours per day for the last year. The CyTOFs are reporting 5090% growth since the last CCSG renewal. These results stem from mitigating the pitfalls, making the technologies approachable and affordable, and supporting the researchers throughout the process all the way to publication. Collaborations, both academic and industry, have resulted to develop or test novel applications. The models we have created to support these technologies have and will continue to be shared with others operating the technologies. We are highly involved with both developing and standardizing new technologies for use at MD Anderson and with our facilities and researchers implementing them worldwide.

项目概要/摘要 流式细胞术、质谱细胞术、细胞分选和成像是多种方法学的重要工具 旨在了解复杂的单细胞蛋白质组和基因组癌症研究。仪器仪表 涉及的内容庞大、复杂，并且可能难以维护和操作。因此，有针对性的核心设施至关重要 对于像 MD 安德森这样的综合癌症中心。 FCCIF 成立于 1982 年，提供这些服务。 自上次 CCSG 更新（2012 年）以来，来自所有 16 个癌症中心项目的 404 名癌症中心成员已使用 该设施并制作了 604 种出版物。目前癌症生物学的许多热门话题都源于免疫 细胞与肿瘤细胞的相互作用；免疫表型分析是一个热门的研究领域，需要独特的方法 高 plex 测定。这需要专门的成像或细胞计数方法，并且 FCCIF 专门提供了 由于其多样性和灵活性，我们收购了两个不同的平台。这些平台是Vectra 多光谱成像系统和 CyTOF（悬浮和成像）质谱细胞仪。 FCCIF 提供 支持医学博士各种研究工作的最先进专业知识和技术技能的共同来源 安德森调查员。 FCCIF 为所有机构调查人员提供全面的服务 无论之前的专业知识如何。 Jared K. Burks 博士（R50 的作者、FCCIF-NC 的联合主任）提供 关键服务包括：1) 教育，2) 所需技术方法的咨询，3) 相关培训 设备使用、分析和正确的实验设计。我识别并获取新技术以供使用 MDACC 调查人员和我以确保成功并减少陷阱的方式提供支持。我预计 开发新的策略和技术来满足我们教师的研究需求。举几个例子说明什么 我目前正在研究的是 Vectra Polaris 多光谱显微镜、Helios 和 Hyperion Suspension 和 CyTOF 成像。去年，Polaris 平均每天运行 13.5 小时。 CyTOF 报告称自上次 CCSG 更新以来增长了 5090%。这些结果源于减少陷阱，使得 技术平易近人且负担得起，并在整个过程中为研究人员提供支持 出版方式。学术界和工业界的合作已经开发或测试了新颖的产品 应用程序。我们为支持这些技术而创建的模型已经并将继续共享 与其他人一起操作这些技术。我们高度参与新产品的开发和标准化 MD 安德森癌症中心以及我们的设施和研究人员在全球范围内实施这些技术。

项目成果

Ataxia-telangiectasia mutated interacts with Parkin and induces mitophagy independent of kinase activity. Evidence from mantle cell lymphoma.

共济失调毛细血管扩张突变与 Parkin 相互作用并诱导独立于激酶活性的线粒体自噬。

• 发表时间: 2021
• 影响因子: 10.1
• 作者: Sarkar, Aloke;Stellrecht, Christine M;Vangapandu, Hima V;Ayres, Mary;Kaipparettu, Benny A;Park, Jun Hyoung;Balakrishnan, Kumudha;Burks, Jared K;Pandita, Tej K;Hittelman, Walter N;Neelapu, Sattva S;Gandhi, Varsha
• 通讯作者: Gandhi, Varsha

SIO: A Spatioimageomics Pipeline to Identify Prognostic Biomarkers Associated with the Ovarian Tumor Microenvironment.

SIO：用于识别与卵巢肿瘤微环境相关的预后生物标志物的空间图像组学管道。

• 发表时间: 2021-04-08
• 影响因子: 5.2
• 作者: Zhu, Ying;Ferri;Sheng, Jianting;Yeung, Tsz;Burks, Jared K;Cappello, Paola;Jazaeri, Amir A;Kim, Jae;Han, Gwan Hee;Birrer, Michael J;Mok, Samuel C;Wong, Stephen T C
• 通讯作者: Wong, Stephen T C

NDRG1 in Aggressive Breast Cancer Progression and Brain Metastasis.

NDRG1 在侵袭性乳腺癌进展和脑转移中的作用。

• 发表时间: 2022-04-11
• 作者: Villodre, Emilly S;Hu, Xiaoding;Eckhardt, Bedrich L;Larson, Richard;Huo, Lei;Yoon, Ester C;Gong, Yun;Song, Juhee;Liu, Shuying;Ueno, Naoto T;Krishnamurthy, Savitri;Pusch, Stefan;Tripathy, Debu;Woodward, Wendy A;Debeb, Bisrat G
• 通讯作者: Debeb, Bisrat G

Correction: Interplay between soluble CD74 and macrophage-migration inhibitory factor drives tumor growth and influences patient survival in melanoma.

更正：可溶性 CD74 和巨噬细胞迁移抑制因子之间的相互作用驱动肿瘤生长并影响黑色素瘤患者的生存。

• 发表时间: 2022-05-02
• 影响因子: 9
• 作者: Fukuda, Yasunari;Bustos, Matias A;Cho, Sung;Roszik, Jason;Ryu, Suyeon;Lopez, Victor M;Burks, Jared K;Lee, Jeffrey E;Grimm, Elizabeth A;Hoon, Dave S B;Ekmekcioglu, Suhendan
• 通讯作者: Ekmekcioglu, Suhendan

Enumeration and molecular characterization of circulating tumor cells enriched by microcavity array from stage III non-small cell lung cancer patients.

通过微腔阵列富集 III 期非小细胞肺癌患者的循环肿瘤细胞的计数和分子特征。

• 发表时间: 2020-10
• 影响因子: 4
• 作者: Cohen, Evan N;Jayachandran, Gitanjali;Gao, Hui;Qiao, Wei;Liu, Suyu;He, Jianzhong;Qiao, Yawei;Yao, Luyang;Lin, Steven H;Reuben, James M
• 通讯作者: Reuben, James M