THE IMPACT OF INSULIN ON THE SHORT-TERM REGULATION OF GHRELIN

胰岛素对 GHRELIN 短期调节的影响

• 批准号: 8167173
• 负责人: RALF M NASS
• 金额: $ 10.02万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167173
• 关键词: Acute; Admission activity; Age; Amino Acids; Biological Assay; Computer Retrieval of Information on Scientific Projects Database; Eating; Elderly; Funding; Gender; Grant; Homeostasis; Infusion procedures; Institution; Insulin; Laboratories; Measures; Mediating; Obesity; Octanoic Acids; Peptides; Plasma; Play; Post-Translational Protein Processing; Protocols documentation; Recruitment Activity; Regulation; Research; Research Personnel; Resources; Role; Saline; Somatotropin; Source; Stomach; Testing; United States National Institutes of Health; Woman; des-n-octanoyl ghrelin; ghrelin; hydroxyl group; insulin sensitivity; men; volunteer; waist circumference; young adult

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ghrelin, a peptide which is mainly derived from the stomach, is present in plasma in an active (acylated) and inactive (des-acylated) form. The major active form of ghrelin contains 28 amino acids with a unique post-translational modification: the hydroxyl group of ser3 is esterified by octanoic acid. The n-octanoyl group is essential for the growth hormone (GH) releasing activity. Plasma ghrelin plays a role in energy homeostasis, food intake, and possibly in GH release and obesity. To date, it is unclear how circulating ghrelin levels are regulated and whether there are age-specific differences in the regulation of ghrelin. Insulin sensitivity decreases with age. The studies proposed will investigate whether there are differences between young and older adults regarding the acute insulin mediated decrease in circulating ghrelin (bioactive, inactive form) levels. We hypothesize that insulin administered under euglycemic conditions will decrease ghrelin to a lesser extent in older adults when compared to young adults. Insulin administered under euglycemic conditions will decrease circulating ghrelin levels to the same extent in older adults as in young adults when matched for BMI, waist circumference and insulin sensitivity. For this purpose, 24 men and women (12 of each gender) age 60 years and older will be recruited. The volunteers have 2 admissions. There is a euglycemic hyperinsulinemic clamp admission and a control admission which has a saline instead of an insulin infusion. The insulin-induced suppression of acyl-and desacyl-ghrelin will be compared to a group of young men and women recruited and tested under another protocol. Using assays developed in the investigators' laboratory, both bioactive and inactive ghrelin will be measured.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 生长素是一种主要源自胃的肽，以活性（酰化）和非活性（DES-酰化）形式存在于血浆中。生长素的主要活性形式含有28种具有独特后翻译后修饰的氨基酸：Ser3的羟基由辛酸酯化。 N-八链酰基基团对于生长激素（GH）释放活性至关重要。血浆生长素素在能量稳态，食物摄入量以及可能在GH释放和肥胖症中发挥作用。迄今为止，尚不清楚如何调节循环的生长素素水平，以及生长素素调节的年龄特异性差异。胰岛素灵敏度随着年龄的增长而降低。提出的研究将研究年轻人和老年人在急性胰岛素介导的循环生长素蛋白（生物活性，非活性形式）水平上是否存在差异。我们假设与年轻人相比，老年人在葡萄糖条件下服用的胰岛素将在老年人的程度较小的程度下降低。 在葡萄糖疾病下服用的胰岛素将使循环肽水平降低到老年人与年轻人相同的程度，而与BMI相匹配时，腰围，腰围和胰岛素敏感性匹配。为此，将招募24名男女60岁及以上的男女。志愿者有2次入学。有一个葡萄糖高胰岛素夹的入院和一个具有盐水而不是胰岛素输注的对照入院。将胰岛素诱导的酰基抑制作用和抗杀菌蛋白的抑制作用将与根据另一种方案招募和测试的一组年轻男女进行比较。使用研究人员实验室中开发的测定，将测量生物活性和非活性生长素素。